C57BL/6JCya-Hebp2em1flox/Cya
Common Name:
Hebp2-flox
Product ID:
S-CKO-11919
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Hebp2-flox
Strain ID
CKOCMP-56016-Hebp2-B6J-VA
Gene Name
Product ID
S-CKO-11919
Gene Alias
SOUL
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
10
Phenotype
Document
Application
--
Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Hebp2em1flox/Cya mice (Catalog S-CKO-11919) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000020000
NCBI RefSeq
NM_019487
Target Region
Exon 1~3
Size of Effective Region
~3.4 kb
Detailed Document
Overview of Gene Research
Hebp2, also known as heme-binding protein 2, has been implicated in multiple biological processes. It may be involved in facilitating necrotic cell death under oxidative stress. Additionally, the interaction between apoptosis-linked gene 2 (ALG-2) and Hebp2 seems to play a role in regulating microtubule cytoskeleton, which impacts mitotic spindle function. This connection is significant as genomic instability in cancer cells can arise from improper microtubule regulation [2].
In triple-negative breast cancer (TNBC), overexpression of Hebp2 enhanced the viability of MDA-MB-231 cells in response to cisplatin and BCNU, while Nuclease technology-mediated Hebp2-knockout reduced cell viability in response to these drugs. This indicates that Hebp2 may play a role in resistance to cisplatin and BCNU, which induce DNA cross-links, in TNBC cells [1].
In ovarian cancer, Hebp2, a fatty acid-associated gene, not only accelerates cancer progression but also drives the polarization from M0_TAM to M2_TAM, modifying the immune landscape and leading to carboplatin resistance. Both in vitro and in vivo experiments showed its role in promoting the proliferation of resistant cell lines and suppressing apoptosis [3].
In conclusion, Hebp2 has functions related to cell death, microtubule regulation, and is involved in cancer drug resistance in breast and ovarian cancers. Studies using gene-editing techniques like Nuclease technology in cell models have been crucial in revealing these functions, providing potential targets for cancer treatment strategies.
References:
1. Kim, Hye Rim, Hong, Jin-Kyung, Kim, Yongsub, Choi, Jeong-Yun. 2024. HEBP2 affects sensitivity to cisplatin and BCNU but not to paclitaxel in MDA-MB-231 breast cancer cells. In Toxicological research, 40, 561-569. doi:10.1007/s43188-024-00249-w. https://pubmed.ncbi.nlm.nih.gov/39345749/
2. Qin, Juan, Yang, Yang, Gao, Siqi, Li, Dengwen, Zhou, Jun. 2017. Deregulated ALG-2/HEBP2 axis alters microtubule dynamics and mitotic spindle behavior to stimulate cancer development. In Journal of cellular physiology, 232, 3067-3076. doi:10.1002/jcp.25754. https://pubmed.ncbi.nlm.nih.gov/28004381/
3. Yang, BiKang, Dai, Miao. 2024. High-dimensional deconstruction of ovarian cancer at single-cell precision reveals HEBP2 that reshape the TIME and drive carboplatin resistance. In Translational oncology, 44, 101917. doi:10.1016/j.tranon.2024.101917. https://pubmed.ncbi.nlm.nih.gov/38554571/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen