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C57BL/6JCya-Tmed2em1flox/Cya
Common Name:
Tmed2-flox
Product ID:
S-CKO-12034
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Price:
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Basic Information
Strain Name
Tmed2-flox
Strain ID
CKOCMP-56334-Tmed2-B6J-VA
Gene Name
Tmed2
Product ID
S-CKO-12034
Gene Alias
1110032D12Rik; 1810020N21Rik; Rnp24; Sid394; p24beta1
Background
C57BL/6JCya
NCBI ID
56334
Modification
Conditional knockout
Chromosome
5
Phenotype
MGI:1929269
Document
Click here to download >>
Application
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More
Rare Disease Data Center >>
Note
Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Tmed2em1flox/Cya mice (Catalog S-CKO-12034) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000060226
NCBI RefSeq
NM_019770
Target Region
Exon 2
Size of Effective Region
~0.7 kb
Detailed Document
Click here to download >>
Overview of Gene Research
TMED2, a member of the transmembrane emp24 domain (TMED) family, is essential for the transport of cargo proteins between the endoplasmic reticulum (ER) and Golgi [7]. It is involved in multiple biological processes such as the formation of plasma membrane lipid nanodomains, regulation of Hedgehog (HH) signaling, and is important for normal morphogenesis of mouse embryos and placenta [1,2,7]. It also plays roles in innate immune responses to DNA viruses by potentiating MITA signaling [9].

In cancer research, TMED2 has been extensively studied. In breast cancer, it is overexpressed and associated with poor prognosis, and it can induce cisplatin resistance via the KEAP1-Nrf2 pathway [3]. In epithelial ovarian cancer, silencing TMED2 decreased cell proliferation, migration, and invasion in vitro and inhibited ovarian cancer growth in mice, as it activates the IGF2/IGF1R/PI3K/Akt pathway [4]. In glioma, TMED2 is essential for cell proliferation, migration, invasion, and tumor formation in mouse models, by enhancing EGFR-AKT signaling through facilitating EGFR recycling [5]. In multiple myeloma cell lines, downregulating TMED2 expression decreased cell viability, altered the cell cycle, and increased apoptosis [8]. In head and neck squamous carcinoma, TMED2 serves as a biomarker for poor prognosis [6].

In conclusion, TMED2 is crucial for protein transport between the ER and Golgi and has far-reaching impacts on various biological processes. Its role in multiple cancer types, as revealed through in vitro and in vivo studies including mouse models, makes it a potential therapeutic target for these malignancies.

References:
1. Anwar, Muhammad U, Sergeeva, Oksana A, Abrami, Laurence, D'Angelo, Giovanni, van der Goot, F Gisou. 2022. ER-Golgi-localized proteins TMED2 and TMED10 control the formation of plasma membrane lipid nanodomains. In Developmental cell, 57, 2334-2346.e8. doi:10.1016/j.devcel.2022.09.004. https://pubmed.ncbi.nlm.nih.gov/36174556/
2. Di Minin, Giulio, Holzner, Markus, Grison, Alice, Roelink, Henk, Wutz, Anton. 2022. TMED2 binding restricts SMO to the ER and Golgi compartments. In PLoS biology, 20, e3001596. doi:10.1371/journal.pbio.3001596. https://pubmed.ncbi.nlm.nih.gov/35353806/
3. Liang, Chen, Zhang, Han-Yong, Wang, Yi-Qian, Zhang, Tong-Cun, Xu, Yao. 2023. TMED2 Induces Cisplatin Resistance in Breast Cancer via Targeting the KEAP1-Nrf2 Pathway. In Current medical science, 43, 1023-1032. doi:10.1007/s11596-023-2777-7. https://pubmed.ncbi.nlm.nih.gov/37615927/
4. Shi-Peng, Gong, Chun-Lin, Chen, Huan, Wu, Guang-Ping, Zhang, Ye-Ping, Cai. 2017. TMED2 promotes epithelial ovarian cancer growth. In Oncotarget, 8, 94151-94165. doi:10.18632/oncotarget.21593. https://pubmed.ncbi.nlm.nih.gov/29212217/
5. Sun, Changning, Zhang, Yihan, Wang, Zhuangzhi, Zhang, Junhua, Gu, Yuchao. 2024. TMED2 promotes glioma tumorigenesis by being involved in EGFR recycling transport. In International journal of biological macromolecules, 262, 130055. doi:10.1016/j.ijbiomac.2024.130055. https://pubmed.ncbi.nlm.nih.gov/38354922/
6. Gao, Wen, Zhang, Zhe-Wen, Wang, Hong-Yi, Liao, Yu-Xuan, Liu, An. 2022. TMED2/9/10 Serve as Biomarkers for Poor Prognosis in Head and Neck Squamous Carcinoma. In Frontiers in genetics, 13, 895281. doi:10.3389/fgene.2022.895281. https://pubmed.ncbi.nlm.nih.gov/35754792/
7. Hou, Wenyang, Jerome-Majewska, Loydie A. 2018. TMED2/emp24 is required in both the chorion and the allantois for placental labyrinth layer development. In Developmental biology, 444, 20-32. doi:10.1016/j.ydbio.2018.09.012. https://pubmed.ncbi.nlm.nih.gov/30236446/
8. Ge, Xueling, Jiang, Wei, Jiang, Yujie, Liu, Xin, Wang, Xin. 2020. Expression and Importance of TMED2 in Multiple Myeloma Cells. In Cancer management and research, 12, 12895-12903. doi:10.2147/CMAR.S278570. https://pubmed.ncbi.nlm.nih.gov/33364837/
9. Sun, Ming-Shun, Zhang, Jie, Jiang, Li-Qun, Shu, Hong-Bing, Liu, Yu. . TMED2 Potentiates Cellular IFN Responses to DNA Viruses by Reinforcing MITA Dimerization and Facilitating Its Trafficking. In Cell reports, 25, 3086-3098.e3. doi:10.1016/j.celrep.2018.11.048. https://pubmed.ncbi.nlm.nih.gov/30540941/
Quality Control Standard
Sperm Test

Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.

Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.

Environmental Standards:SPF
Available Region:Global
Source:Cyagen
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