C57BL/6JCya-Snd1em1flox/Cya
Common Name:
Snd1-flox
Product ID:
S-CKO-12120
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
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Basic Information
Strain Name
Snd1-flox
Strain ID
CKOCMP-56463-Snd1-B6J-VA
Gene Name
Product ID
S-CKO-12120
Gene Alias
Tudor-SN
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
6
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Snd1em1flox/Cya mice (Catalog S-CKO-12120) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000001460
NCBI RefSeq
NM_019776
Target Region
Exon 5
Size of Effective Region
~1.0 kb
Detailed Document
Overview of Gene Research
Snd1, also known as Tudor-SN, TSN or p100, is an evolutionarily conserved protein. It contains four staphylococcal nuclease domains and a Tudor domain, endowing it with endonuclease activity and strong nucleic acid-protein interaction capabilities. Snd1 is involved in multiple gene expression regulatory processes such as RNA splicing, interference, stability, and editing, as well as protein and lipid homeostasis regulation. It has been associated with various biological processes and diseases, and genetic models are valuable for studying its functions [5].
In cancer research, Snd1 is highly expressed in most cancers and its expression is related to patient prognosis. For example, in breast cancer, disrupting the MTDH-SND1 complex using small-molecule inhibitors can suppress tumor growth and metastasis, and enhance chemotherapy sensitivity and immune surveillance [2,3,4]. In prostate cancer, knocking down Snd1 in human prostate epithelial cells impacts cell proliferation, and prostate-specific Snd1 deletion in mice reduces cancer growth [6]. In esophageal squamous cell carcinoma, loss of KDM6A or Snd1 leads to enhanced cellular sensitivity to genotoxins [8]. In the study of SARS-CoV-2, Snd1 binds to the 5' end of negative-sense viral RNA and is required for viral RNA synthesis, with Snd1-depleted cells showing smaller replication organelles and diminished virus growth kinetics [1]. In lipid metabolism, Snd1 plays a direct role in regulating cholesterol metabolism by affecting SREBP2 activation [7]. Also, Snd1 knockout mice show decreased fertility, organ and body size, and changes in gene expression similar to adaptation to hypoxia [9].
In summary, Snd1 is crucial in multiple biological processes and diseases. Gene knockout and conditional knockout mouse models have revealed its roles in cancer progression, viral RNA synthesis, lipid metabolism, and hypoxia adaptation. These findings provide important insights into understanding disease mechanisms and developing potential therapeutic strategies related to Snd1-associated functions.
References：
1. Schmidt, Nora, Ganskih, Sabina, Wei, Yuanjie, Erhard, Florian, Munschauer, Mathias. 2023. SND1 binds SARS-CoV-2 negative-sense RNA and promotes viral RNA synthesis through NSP9. In Cell, 186, 4834-4850.e23. doi:10.1016/j.cell.2023.09.002. https://pubmed.ncbi.nlm.nih.gov/37794589/
2. Cui, Xiaoteng, Zhang, Xinxin, Liu, Minghui, Gao, Xingjie, Yang, Jie. 2020. A pan-cancer analysis of the oncogenic role of staphylococcal nuclease domain-containing protein 1 (SND1) in human tumors. In Genomics, 112, 3958-3967. doi:10.1016/j.ygeno.2020.06.044. https://pubmed.ncbi.nlm.nih.gov/32645525/
3. Shen, Minhong, Wei, Yong, Kim, Hahn, Shao, Zhi-Ming, Kang, Yibin. 2021. Small-molecule inhibitors that disrupt the MTDH-SND1 complex suppress breast cancer progression and metastasis. In Nature cancer, 3, 43-59. doi:10.1038/s43018-021-00279-5. https://pubmed.ncbi.nlm.nih.gov/35121987/
4. Shen, Minhong, Smith, Heath A, Wei, Yong, Shao, Zhi-Ming, Kang, Yibin. 2021. Pharmacological disruption of the MTDH-SND1 complex enhances tumor antigen presentation and synergizes with anti-PD-1 therapy in metastatic breast cancer. In Nature cancer, 3, 60-74. doi:10.1038/s43018-021-00280-y. https://pubmed.ncbi.nlm.nih.gov/35121988/
5. Ochoa, Begoña, Chico, Yolanda, Martínez, María José. 2018. Insights Into SND1 Oncogene Promoter Regulation. In Frontiers in oncology, 8, 606. doi:10.3389/fonc.2018.00606. https://pubmed.ncbi.nlm.nih.gov/30619748/
6. Liao, Sheng-You, Rudoy, Dmytro, Frank, Sander B, Emili, Andrew, Vasioukhin, Valeri. 2023. SND1 binds to ERG and promotes tumor growth in genetic mouse models of prostate cancer. In Nature communications, 14, 7435. doi:10.1038/s41467-023-43245-8. https://pubmed.ncbi.nlm.nih.gov/37973913/
7. Navarro-Imaz, Hiart, Ochoa, Begoña, García-Arcos, Itsaso, Fresnedo, Olatz, Rueda, Yuri. 2020. Molecular and cellular insights into the role of SND1 in lipid metabolism. In Biochimica et biophysica acta. Molecular and cell biology of lipids, 1865, 158589. doi:10.1016/j.bbalip.2019.158589. https://pubmed.ncbi.nlm.nih.gov/31978555/
8. Wu, Jian, Jiang, Yixin, Zhang, Qin, Qiu, Lei, Han, Junhong. . KDM6A-SND1 interaction maintains genomic stability by protecting the nascent DNA and contributes to cancer chemoresistance. In Nucleic acids research, 52, 7665-7686. doi:10.1093/nar/gkae487. https://pubmed.ncbi.nlm.nih.gov/38850159/
9. Saarikettu, Juha, Lehmusvaara, Saara, Pesu, Marko, Haikarainen, Teemu, Silvennoinen, Olli. 2023. The RNA-binding protein Snd1/Tudor-SN regulates hypoxia-responsive gene expression. In FASEB bioAdvances, 5, 183-198. doi:10.1096/fba.2022-00115. https://pubmed.ncbi.nlm.nih.gov/37151849/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen