C57BL/6JCya-Srsf4em1flox/Cya
Common Name:
Srsf4-flox
Product ID:
S-CKO-12303
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Srsf4-flox
Strain ID
CKOCMP-57317-Srsf4-B6J-VA
Gene Name
Product ID
S-CKO-12303
Gene Alias
5730499P16Rik; MNCb-2616; SRp75; Sfrs4
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
4
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Srsf4em1flox/Cya mice (Catalog S-CKO-12303) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000053819
NCBI RefSeq
NM_020587
Target Region
Exon 2
Size of Effective Region
~1.1 kb
Detailed Document
Overview of Gene Research
Srsf4, a member of the serine-/arginine (SR)-rich protein family, is involved in both constitutive and alternative splicing. It contains multiple nuclear localization sequences and is localized in the nucleus with a speckled pattern [2]. Srsf4 is an essential component of spliceosomes, participating in processes like alternative splicing, spliceosome assembly, mRNA export, and nonsense-mediated mRNA decay, which are crucial for maintaining cellular and tissue homeostasis [5].
Srsf4 has been associated with various disease conditions. In glioma, its expression is related to histological grade, IDH1 status, and prognosis. Srsf4 promotes temozolomide resistance by positively regulating MDC1 and accelerating double-strand break repair, and targeting it can improve chemosensitivity [1]. In pulmonary hypertension, Srsf4 upregulates the expression of ca-circFOXP1 by splicing exons 6 and 9 of FOXP1 pre-mRNA, which then regulates hypoxia-induced PASMC proliferation [3]. In patients with a missense variant of the Srsf4 gene, lower Srsf4 protein expression leads to impaired mitochondrial function and marrow failure [4].
In conclusion, Srsf4 plays a vital role in RNA splicing-related processes and is implicated in multiple diseases such as glioma, pulmonary hypertension, and bone marrow failure. Research on Srsf4, including through gene-related models, helps to understand the underlying molecular mechanisms of these diseases, potentially providing new directions for diagnosis, prognosis, and treatment.
References:
1. Sun, Yi, Liu, Xingdong, Wu, Zhiqiang, Yan, Wei, You, Yongping. 2023. SRSF4 Confers Temozolomide Resistance of Glioma via Accelerating Double Strand Break Repair. In Journal of molecular neuroscience : MN, 73, 259-268. doi:10.1007/s12031-023-02115-0. https://pubmed.ncbi.nlm.nih.gov/37014544/
2. Masaki, So, Kabuto, Takafumi, Suzuki, Kenji, Kataoka, Naoyuki. 2020. Multiple nuclear localization sequences in SRSF4 protein. In Genes to cells : devoted to molecular & cellular mechanisms, 25, 327-333. doi:10.1111/gtc.12756. https://pubmed.ncbi.nlm.nih.gov/32050040/
3. Song, Xinyue, Xu, Ya, Li, Mengnan, Zheng, Xiaodong, Zhu, Daling. 2025. SRSF4-Associated ca-circFOXP1 Regulates Hypoxia-Induced PASMC Proliferation by the Formation of R Loop With Host Gene. In Arteriosclerosis, thrombosis, and vascular biology, 45, e118-e135. doi:10.1161/ATVBAHA.124.322026. https://pubmed.ncbi.nlm.nih.gov/39973750/
4. Miano, Maurizio, Bertola, Nadia, Grossi, Alice, Cappelli, Enrico, Ravera, Silvia. 2024. Impaired Mitochondrial Function and Marrow Failure in Patients Carrying a Variant of the SRSF4 Gene. In International journal of molecular sciences, 25, . doi:10.3390/ijms25042083. https://pubmed.ncbi.nlm.nih.gov/38396760/
5. Tan, Wanyan, Wang, Wei, Ma, Qingfeng. 2018. Physiological and Pathological Function of Serine/Arginine-Rich Splicing Factor 4 and Related Diseases. In BioMed research international, 2018, 3819719. doi:10.1155/2018/3819719. https://pubmed.ncbi.nlm.nih.gov/29789787/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen