C57BL/6JCya-Serbp1em1flox/Cya
Common Name:
Serbp1-flox
Product ID:
S-CKO-13351
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Serbp1-flox
Strain ID
CKOCMP-66870-Serbp1-B6J-VA
Gene Name
Product ID
S-CKO-13351
Gene Alias
1200009K13Rik; 9330147J08Rik; Pairbp1
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
6
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Serbp1em1flox/Cya mice (Catalog S-CKO-13351) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000042990
NCBI RefSeq
NM_025814
Target Region
Exon 2~5
Size of Effective Region
~2.2 kb
Detailed Document
Overview of Gene Research
Serbp1, also known as SERPINE1 mRNA binding protein 1, is an RNA binding protein that lacks canonical RNA-binding domains and is mostly disordered [2,5]. It plays crucial roles in multiple biological processes. SERBP1 is involved in pathways such as stress granule clearance, splicing, cell division, ribosome biogenesis, apoptosis regulation, and ferroptosis regulation [1-4]. It is also associated with the DNA damage response, mRNA metabolism, and translation regulation, making it biologically important in normal cell function and disease-related processes [7,8,9].
In vivo, depletion of SERBP1 in testicular cells leads to increased germ cell apoptosis upon scrotal heat stress, suggesting its role in protecting male germ cells from thermostimuli damage. It does this by regulating 26S proteasome activity and G3BP1 ubiquitination to facilitate stress granule clearance [1]. In HeLa cells, overexpression of SERBP1 induces apoptosis, with downregulated genes enriched in cell proliferation and apoptosis pathways, and genes with SERBP1-regulated alternative splicing enriched in transcriptional regulation and metabolic processes related to tumorigenesis [3]. KSHV-encoded vIL-6 promotes SIRT3-induced deacetylation of SERBP1, which inhibits ferroptosis and enhances cellular transformation [4]. In neurons, silencing of SERBP1 relieves KCC2 repression by miR-92 [6]. SERBP1 also affects homologous recombination-mediated DNA repair by regulating CtIP translation during S phase [8].
In conclusion, SERBP1 is a multifunctional protein involved in stress granule clearance, cell apoptosis, ferroptosis regulation, and DNA repair. Its role in protecting male germ cells from heat-induced apoptosis, as well as its involvement in tumor-related processes like apoptosis and metabolic regulation in HeLa cells, and KSHV-induced cellular transformation, highlight its importance in disease-related research. The study of SERBP1 using in vivo models helps in understanding its complex functions and provides potential targets for disease treatment.
References:
1. Wang, Fengli, Wang, Lingjuan, Gan, Shiming, Wang, Xiaoli, Yuan, Shuiqiao. 2023. SERBP1 Promotes Stress Granule Clearance by Regulating 26S Proteasome Activity and G3BP1 Ubiquitination and Protects Male Germ Cells from Thermostimuli Damage. In Research (Washington, D.C.), 6, 0091. doi:10.34133/research.0091. https://pubmed.ncbi.nlm.nih.gov/37223481/
2. Breunig, Kira, Lei, Xiufen, Montalbano, Mauro, Galante, Pedro A F, Penalva, Luiz O F. 2024. SERBP1 interacts with PARP1 and is present in PARylation-dependent protein complexes regulating splicing, cell division, and ribosome biogenesis. In bioRxiv : the preprint server for biology, , . doi:10.1101/2024.03.22.586270. https://pubmed.ncbi.nlm.nih.gov/38585848/
3. Zhou, Junjie, Chen, Wenhao, He, Qianwen, Ding, Zhao, Qian, Qun. 2022. SERBP1 affects the apoptotic level by regulating the expression and alternative splicing of cellular and metabolic process genes in HeLa cells. In PeerJ, 10, e14084. doi:10.7717/peerj.14084. https://pubmed.ncbi.nlm.nih.gov/36213507/
4. Zhou, Jing, Wang, Tianjiao, Zhang, Haoran, Gao, Shou-Jiang, Lu, Chun. 2024. KSHV vIL-6 promotes SIRT3-induced deacetylation of SERBP1 to inhibit ferroptosis and enhance cellular transformation by inducing lipoyltransferase 2 mRNA degradation. In PLoS pathogens, 20, e1012082. doi:10.1371/journal.ppat.1012082. https://pubmed.ncbi.nlm.nih.gov/38470932/
5. Breunig, Kira, Lei, Xuifen, Montalbano, Mauro, Galante, Pedro A F, Penalva, Luiz O. 2025. SERBP1 interacts with PARP1 and is present in PARylation-dependent protein complexes regulating splicing, cell division, and ribosome biogenesis. In eLife, 13, . doi:10.7554/eLife.98152. https://pubmed.ncbi.nlm.nih.gov/39937575/
6. Barbato, Christian, Frisone, Paola, Braccini, Laura, Cogoni, Carlo, Ruberti, Francesca. 2022. Silencing of Ago-2 Interacting Protein SERBP1 Relieves KCC2 Repression by miR-92 in Neurons. In Cells, 11, . doi:10.3390/cells11061052. https://pubmed.ncbi.nlm.nih.gov/35326503/
7. Colleti, Carolina, Melo-Hanchuk, Talita Diniz, da Silva, Flávia Regina Moraes, Saito, Ângela, Kobarg, Jörg. . Complex interactomes and post-translational modifications of the regulatory proteins HABP4 and SERBP1 suggest pleiotropic cellular functions. In World journal of biological chemistry, 10, 44-64. doi:10.4331/wjbc.v10.i3.44. https://pubmed.ncbi.nlm.nih.gov/31768228/
8. Ahn, Jang-Won, Kim, Sunjik, Na, Wooju, Kim, Seon-Young, Choi, Cheol Yong. 2015. SERBP1 affects homologous recombination-mediated DNA repair by regulation of CtIP translation during S phase. In Nucleic acids research, 43, 6321-33. doi:10.1093/nar/gkv592. https://pubmed.ncbi.nlm.nih.gov/26068472/
9. Martini, Silvia, Davis, Khalil, Faraway, Rupert, Ule, Jernej, Parker, Peter J. 2021. A genetically-encoded crosslinker screen identifies SERBP1 as a PKCε substrate influencing translation and cell division. In Nature communications, 12, 6934. doi:10.1038/s41467-021-27189-5. https://pubmed.ncbi.nlm.nih.gov/34836941/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen