C57BL/6JCya-Zc3h13em1flox/Cya
Common Name
Zc3h13-flox
Product ID
S-CKO-13619
Backgroud
C57BL/6JCya
Strain ID
CKOCMP-67302-Zc3h13-B6J-VA
When using this mouse strain in a publication, please cite “Zc3h13-flox Mouse (Catalog S-CKO-13619) were purchased from Cyagen.”
Product Type
Age
Genotype
Sex
Quantity
Basic Information
Strain Name
Zc3h13-flox
Strain ID
CKOCMP-67302-Zc3h13-B6J-VA
Gene Name
Product ID
S-CKO-13619
Gene Alias
2600010B19Rik, 3110050K21Rik, 4930570G11Rik
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
Chr 14
Phenotype
Datasheet
Application
--
Strain Description
Ensembl Number
ENSMUST00000022577
NCBI RefSeq
NM_026083
Target Region
Exon 4
Size of Effective Region
~1.3 kb
Overview of Gene Research
Zc3h13, a zinc-finger protein, is an important regulator in the m6A methylation machinery. N6-methyladenosine (m6A) is a prevalent modification in eukaryotic mRNA, influencing mRNA stability, splicing, export, and translation. Zc3h13 is part of the "writers" group of m6A regulators, which install m6A modifications. It plays a crucial role in multiple biological processes and is associated with various diseases, highlighting its overall biological importance [1,3].
Knockdown of Zc3h13 in mouse embryonic stem cells significantly decreases global m6A levels on mRNA. It is required for the nuclear localization of the Zc3h13-WTAP-Virilizer-Hakai complex, which is essential for RNA m6A methylation. Depletion of Zc3h13 impairs self-renewal and triggers mESC differentiation [1]. In cervical cancer, Zc3h13-mediated m6A modification of CENPK mRNA promotes cancer stemness and chemoresistance [2]. In papillary thyroid carcinoma, overexpressed Zc3h13 suppresses tumor growth through m6A modification-mediated IQGAP1 degradation [4]. In laryngeal squamous cell carcinoma, Zc3h13 reduces DUOX1-mediated ferroptosis through m6A-dependent modification [5]. In colorectal cancer, Zc3h13 acts as a tumor suppressor, inactivating the Ras-ERK signaling pathway [6]. In keloid formation, Zc3h13 accelerates the process by mediating the m6A modification of HIPK2 [7]. In acute myocardial infarction, ZC3H13-mediated m6A modification ameliorates the condition by preventing inflammation, oxidative stress, and ferroptosis [8].
In conclusion, Zc3h13 is essential for m6A methylation in the nucleus, regulating processes like embryonic stem cell self-renewal. Its role in various disease conditions such as cancer and cardiac diseases has been revealed through model-based research, especially gene-knockout studies. Understanding Zc3h13's functions provides insights into disease mechanisms and potential therapeutic targets for these diseases.
References：
1. Wen, Jing, Lv, Ruitu, Ma, Honghui, Shi, Yang, Diao, Jianbo. . Zc3h13 Regulates Nuclear RNA m6A Methylation and Mouse Embryonic Stem Cell Self-Renewal. In Molecular cell, 69, 1028-1038.e6. doi:10.1016/j.molcel.2018.02.015. https://pubmed.ncbi.nlm.nih.gov/29547716/
2. Lin, Xian, Wang, Feng, Chen, Jian, Chen, Chuan-Ben, Xu, Qin. 2022. N6-methyladenosine modification of CENPK mRNA by ZC3H13 promotes cervical cancer stemness and chemoresistance. In Military Medical Research, 9, 19. doi:10.1186/s40779-022-00378-z. https://pubmed.ncbi.nlm.nih.gov/35418160/
3. Wang, Tianyi, Kong, Shan, Tao, Mei, Ju, Shaoqing. 2020. The potential role of RNA N6-methyladenosine in Cancer progression. In Molecular cancer, 19, 88. doi:10.1186/s12943-020-01204-7. https://pubmed.ncbi.nlm.nih.gov/32398132/
4. Xie, Rong, Chen, Wanzhi, Lv, Yunxia, Xiong, Chengfeng, Yu, Jichun. 2023. Overexpressed ZC3H13 suppresses papillary thyroid carcinoma growth through m6A modification-mediated IQGAP1 degradation. In Journal of the Formosan Medical Association = Taiwan yi zhi, 122, 738-746. doi:10.1016/j.jfma.2022.12.019. https://pubmed.ncbi.nlm.nih.gov/36739231/
5. Huang, Lili, Chen, Guangli, He, Jing, Wang, Pu. 2023. ZC3H13 reduced DUOX1-mediated ferroptosis in laryngeal squamous cell carcinoma cells through m6A-dependent modification. In Tissue & cell, 84, 102187. doi:10.1016/j.tice.2023.102187. https://pubmed.ncbi.nlm.nih.gov/37536262/
6. Zhu, Dehua, Zhou, Jianping, Zhao, Jinbo, Zhang, Yong, Dong, Ming. 2018. ZC3H13 suppresses colorectal cancer proliferation and invasion via inactivating Ras-ERK signaling. In Journal of cellular physiology, 234, 8899-8907. doi:10.1002/jcp.27551. https://pubmed.ncbi.nlm.nih.gov/30311220/
7. Fu, Manni, Chen, Yongjun, Shi, Xian. 2023. ZC3H13 Accelerates Keloid Formation by Mediating N6-methyladenosine Modification of HIPK2. In Biochemical genetics, 62, 1857-1871. doi:10.1007/s10528-023-10514-6. https://pubmed.ncbi.nlm.nih.gov/37752292/
8. Cai, Jiumei, Wang, Xiaoping, Wang, Ziliang, Tang, Fosheng, Zhang, Zhiwei. 2024. ZC3H13-Mediated m6A Modification Ameliorates Acute Myocardial Infarction through Preventing Inflammation, Oxidative Stress and Ferroptosis by Targeting lncRNA93358. In Inflammation, , . doi:10.1007/s10753-024-02116-0. https://pubmed.ncbi.nlm.nih.gov/39107569/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen
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