C57BL/6NCya-Mettl16em1flox/Cya
Common Name
Mettl16-flox
Product ID
S-CKO-13725
Backgroud
C57BL/6NCya
Strain ID
CKOCMP-67493-Mettl16-B6N-VA
When using this mouse strain in a publication, please cite “Mettl16-flox Mouse (Catalog S-CKO-13725) were purchased from Cyagen.”
Product Type
Age
Genotype
Sex
Quantity
Basic Information
Strain Name
Mettl16-flox
Strain ID
CKOCMP-67493-Mettl16-B6N-VA
Gene Name
Product ID
S-CKO-13725
Gene Alias
2610100D03Rik, 2810013M15Rik, A830095F14Rik, Mett10d
Background
C57BL/6NCya
NCBI ID
Modification
Conditional knockout
Chromosome
Chr 11
Phenotype
Datasheet
Application
--
Strain Description
Ensembl Number
ENSMUST00000141755
NCBI RefSeq
NM_026197
Target Region
Exon 3
Size of Effective Region
~0.9 kb
Overview of Gene Research
METTL16, an atypical methyltransferase, is responsible for depositing a vertebrate-conserved S-adenosylmethionine site. It functions in RNA modification, playing roles in processes like regulating SAM synthetase intron retention and affecting m6A modification in various RNAs. It is involved in multiple biological pathways and is essential for most cells [2,5].
Genetic depletion of METTL16 in mice has shown significant impacts. In acute myeloid leukemia (AML), it suppresses AML initiation/development and LSC/LIC self-renewal [1]. In hepatocellular carcinoma, liver-specific Mettl16 conditional KO (cKO) mice had attenuated HCC initiation and progression, and METTL16 depletion decreased liver CSC frequency [3]. In pancreatic ductal adenocarcinoma (PDAC), METTL16's role in homologous recombination repair was revealed, with high METTL16-expressing PDAC cells being more sensitive to PARPi [4].
In conclusion, METTL16 is crucial in multiple biological processes and disease conditions. KO/CKO mouse models have been instrumental in uncovering its roles in cancers such as AML, HCC, and PDAC, highlighting its potential as a therapeutic target in these disease areas.
References：
1. Han, Li, Dong, Lei, Leung, Keith, Chen, Jianjun, Deng, Xiaolan. . METTL16 drives leukemogenesis and leukemia stem cell self-renewal by reprogramming BCAA metabolism. In Cell stem cell, 30, 52-68.e13. doi:10.1016/j.stem.2022.12.006. https://pubmed.ncbi.nlm.nih.gov/36608679/
2. Pendleton, Kathryn E, Chen, Beibei, Liu, Kuanqing, Tu, Benjamin P, Conrad, Nicholas K. . The U6 snRNA m6A Methyltransferase METTL16 Regulates SAM Synthetase Intron Retention. In Cell, 169, 824-835.e14. doi:10.1016/j.cell.2017.05.003. https://pubmed.ncbi.nlm.nih.gov/28525753/
3. Xue, Meilin, Dong, Lei, Zhang, Honghai, Chen, Hao, Su, Rui. 2024. METTL16 promotes liver cancer stem cell self-renewal via controlling ribosome biogenesis and mRNA translation. In Journal of hematology & oncology, 17, 7. doi:10.1186/s13045-024-01526-9. https://pubmed.ncbi.nlm.nih.gov/38302992/
4. Zeng, Xiangyu, Zhao, Fei, Cui, Gaofeng, Tao, Kaixiong, Lou, Zhenkun. 2022. METTL16 antagonizes MRE11-mediated DNA end resection and confers synthetic lethality to PARP inhibition in pancreatic ductal adenocarcinoma. In Nature cancer, 3, 1088-1104. doi:10.1038/s43018-022-00429-3. https://pubmed.ncbi.nlm.nih.gov/36138131/
5. Satterwhite, Emily R, Mansfield, Kyle D. 2021. RNA methyltransferase METTL16: Targets and function. In Wiley interdisciplinary reviews. RNA, 13, e1681. doi:10.1002/wrna.1681. https://pubmed.ncbi.nlm.nih.gov/34227247/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen
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