C57BL/6JCya-Tmem86aem1flox/Cya
Common Name
Tmem86a-flox
Product ID
S-CKO-13946
Backgroud
C57BL/6JCya
Strain ID
CKOCMP-67893-Tmem86a-B6J-VA
When using this mouse strain in a publication, please cite “Tmem86a-flox Mouse (Catalog S-CKO-13946) were purchased from Cyagen.”
Product Type
Age
Genotype
Sex
Quantity
Basic Information
Strain Name
Tmem86a-flox
Strain ID
CKOCMP-67893-Tmem86a-B6J-VA
Gene Name
Product ID
S-CKO-13946
Gene Alias
1810054O13Rik
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
Chr 7
Phenotype
Datasheet
Application
--
Strain Description
Ensembl Number
ENSMUST00000010451
NCBI RefSeq
NM_026436
Target Region
Exon 2~3
Size of Effective Region
~2.7 kb
Overview of Gene Research
TMEM86A, also known as transmembrane protein 86A, is a putative lysoplasmalogenase. Lysoplasmalogens are key in plasmalogen metabolism and membrane fluidity. TMEM86A is involved in regulating plasmalogen homeostasis and is associated with protein kinase A-dependent energy metabolism pathways. Its study using genetic models can help understand its role in various biological processes and diseases [1,2].
In adipocyte-specific TMEM86A-knockout (AKO) mice, lysoplasmalogen content in adipose tissue increases, accompanied by an elevation in protein kinase A signalling pathways due to phosphodiesterase 3B inhibition and cyclic adenosine monophosphate elevation. This leads to upregulated mitochondrial oxidative metabolism, increased energy expenditure, and protection from high-fat diet-induced metabolic dysfunction [1].
In macrophages, TMEM86a (a related form) is a sterol-regulated lysoplasmalogenase. Overexpression in bone marrow-derived macrophages (BMDMs) reduces lysoplasmalogen abundance and membrane fluidity, while silencing increases basal lysoplasmalogen levels and abolishes liver X receptor (LXR)-dependent reduction of this lipid species [2].
In conclusion, TMEM86A is crucial for regulating plasmalogen homeostasis and energy metabolism. Studies using KO mouse models have revealed its potential as a therapeutic target for obesity-related metabolic diseases. In macrophages, it contributes to sterol-dependent membrane remodeling. Understanding TMEM86A provides insights into lipid metabolism and related disease mechanisms [1,2].
References：
1. Cho, Yoon Keun, Yoon, Young Cheol, Im, Hyeonyeong, Kwon, Sung Won, Lee, Yun-Hee. 2022. Adipocyte lysoplasmalogenase TMEM86A regulates plasmalogen homeostasis and protein kinase A-dependent energy metabolism. In Nature communications, 13, 4084. doi:10.1038/s41467-022-31805-3. https://pubmed.ncbi.nlm.nih.gov/35835749/
2. van Wouw, Suzanne A E, van den Berg, Marlene, El Ouraoui, Maroua, de Winther, Menno P J, Zelcer, Noam. 2022. Sterol-regulated transmembrane protein TMEM86a couples LXR signaling to regulation of lysoplasmalogens in macrophages. In Journal of lipid research, 64, 100325. doi:10.1016/j.jlr.2022.100325. https://pubmed.ncbi.nlm.nih.gov/36592658/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen
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