C57BL/6JCya-Rnmtem1flox/Cya
Common Name:
Rnmt-flox
Product ID:
S-CKO-13950
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Rnmt-flox
Strain ID
CKOCMP-67897-Rnmt-B6J-VA
Gene Name
Product ID
S-CKO-13950
Gene Alias
2610002P10Rik; Rg7mt1; mKIAA0398
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
18
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Rnmtem1flox/Cya mice (Catalog S-CKO-13950) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000009679
NCBI RefSeq
NM_026440
Target Region
Exon 3
Size of Effective Region
~1.4 kb
Detailed Document
Overview of Gene Research
Rnmt, short for RNA guanine-7 methyltransferase, is a crucial enzyme in RNA cap formation. It catalyzes the N7 methylation of the cap guanosine, a step essential for creating translation-competent mRNA. This process is central to eukaryotic gene expression, as the RNA cap recruits factors involved in splicing, nuclear export, and translation initiation [1,2,3,4,5,6,7].
During neural differentiation, repression of the RNMT complex (RNMT-RAM) is required for the repression of pluripotency-associated gene products [1]. In T cell activation, RNMT is induced by TCR stimulation and coordinates the mRNA, snoRNA, and rRNA production needed for ribosome biogenesis. Rnmt cKO CD4 T cells show decreased ribosome synthesis, reduced translation rates, and proliferation failure [2]. In breast cancer cells, a subset with oncogenic mutations in PIK3CA is highly dependent on RNMT for proliferation. Reducing RNMT activity by 50% leads to decreased proliferation and increased apoptosis in these cells [6].
In summary, Rnmt plays a vital role in multiple biological processes such as cell differentiation, T cell activation, and cell proliferation. Its function has been clearly demonstrated through conditional knockout (cKO) mouse models in the context of neural differentiation, T cell activation, and breast cancer. Understanding Rnmt's function provides insights into normal biological processes and potential therapeutic targets for related diseases.
References:
1. Liang, Shang, Almohammed, Rajaei, Cowling, Victoria H. . The RNA cap methyltransferases RNMT and CMTR1 co-ordinate gene expression during neural differentiation. In Biochemical Society transactions, 51, 1131-1141. doi:10.1042/BST20221154. https://pubmed.ncbi.nlm.nih.gov/37145036/
2. Galloway, Alison, Kaskar, Aneesa, Ditsova, Dimitrinka, Jemielity, Jacek, Cowling, Victoria H. . Upregulation of RNA cap methyltransferase RNMT drives ribosome biogenesis during T cell activation. In Nucleic acids research, 49, 6722-6738. doi:10.1093/nar/gkab465. https://pubmed.ncbi.nlm.nih.gov/34125914/
3. Varshney, Dhaval, Lombardi, Olivia, Schweikert, Gabriele, Suska, Olga, Cowling, Victoria H. . mRNA Cap Methyltransferase, RNMT-RAM, Promotes RNA Pol II-Dependent Transcription. In Cell reports, 23, 1530-1542. doi:10.1016/j.celrep.2018.04.004. https://pubmed.ncbi.nlm.nih.gov/29719263/
4. Aregger, Michael, Kaskar, Aneesa, Varshney, Dhaval, Weidlich, Simone, Cowling, Victoria H. . CDK1-Cyclin B1 Activates RNMT, Coordinating mRNA Cap Methylation with G1 Phase Transcription. In Molecular cell, 61, 734-746. doi:10.1016/j.molcel.2016.02.008. https://pubmed.ncbi.nlm.nih.gov/26942677/
5. Osborne, Michael J, Volpon, Laurent, Memarpoor-Yazdi, Mina, Cowling, Victoria H, Borden, Katherine L B. 2022. Identification and Characterization of the Interaction Between the Methyl-7-Guanosine Cap Maturation Enzyme RNMT and the Cap-Binding Protein eIF4E. In Journal of molecular biology, 434, 167451. doi:10.1016/j.jmb.2022.167451. https://pubmed.ncbi.nlm.nih.gov/35026230/
6. Dunn, Sianadh, Lombardi, Olivia, Lukoszek, Radoslaw, Cowling, Victoria H. . Oncogenic PIK3CA mutations increase dependency on the mRNA cap methyltransferase, RNMT, in breast cancer cells. In Open biology, 9, 190052. doi:10.1098/rsob.190052. https://pubmed.ncbi.nlm.nih.gov/30991934/
7. Aregger, Michael, Cowling, Victoria H. . Human cap methyltransferase (RNMT) N-terminal non-catalytic domain mediates recruitment to transcription initiation sites. In The Biochemical journal, 455, 67-73. doi:10.1042/BJ20130378. https://pubmed.ncbi.nlm.nih.gov/23863084/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen