C57BL/6JCya-Wdfy1em1flox/Cya
Common Name
Wdfy1-flox
Product ID
S-CKO-14507
Backgroud
C57BL/6JCya
Strain ID
CKOCMP-69368-Wdfy1-B6J-VA
When using this mouse strain in a publication, please cite “Wdfy1-flox Mouse (Catalog S-CKO-14507) were purchased from Cyagen.”
Product Type
Age
Genotype
Sex
Quantity
Basic Information
Strain Name
Wdfy1-flox
Strain ID
CKOCMP-69368-Wdfy1-B6J-VA
Gene Name
Product ID
S-CKO-14507
Gene Alias
1700013B03Rik, 1700120F24Rik, FENS-1, Jr1, WDF1, ZFYVE17, mKIAA1435
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
Chr 1
Phenotype
Datasheet
Application
--
Strain Description
Ensembl Number
ENSMUST00000113512
NCBI RefSeq
NM_001111279
Target Region
Exon 2
Size of Effective Region
~1.2 kb
Overview of Gene Research
Wdfy1, the WD repeat and FYVE domain containing 1 gene, encodes a 410-amino acid protein with 97.8% amino acid sequence identity to the human WDFY1 protein [1]. It is involved in crucial biological processes such as the TLR3/4 signaling pathway in the innate immune system, where it mediates the recruitment of TRIF to TLR3 and TLR4, potentiating the activation of NF-κB, IRF3, and production of type I interferons and inflammatory cytokines [3]. It also plays a role in lysophagy initiation as part of the CUL4A-DDB1-WDFY1 E3 ubiquitin ligase complex [4].
Global Wdfy1 knockout (KO) male mice show normal growth, development, and fertility, indicating that WDFY1 is dispensable for mouse spermatogenesis and male fertility [1]. Wdfy1-deficient mice display impaired Tlr3-mediated immune responses in vivo [2]. In a cGVHD mouse model, WDFY1-expressing follicular dendritic cells are critical for lupus development as Wdfy1 knockout impairs the normal functioning of FDCs, resulting in reduced auto-Ab response to cGVHD [5].
In conclusion, Wdfy1 has essential functions in the innate immune response and lysophagy. The gene knockout mouse models have revealed its non-essential role in spermatogenesis and male fertility, as well as its importance in certain immune-related disease conditions like Tlr3-mediated immune responses and lupus development.
References：
1. Lv, Chunyu, Xiong, Mengneng, Guo, Shuangshuang, Qin, Weibing, Yuan, Shuiqiao. 2022. WDFY1, a WD40 repeat protein, is not essential for spermatogenesis and male fertility in mice. In Biochemical and biophysical research communications, 596, 71-75. doi:10.1016/j.bbrc.2022.01.084. https://pubmed.ncbi.nlm.nih.gov/35121371/
2. Yang, Yang, Hu, Yun-Hong, Liu, Yu. 2020. Wdfy1 deficiency impairs Tlr3-mediated immune responses in vivo. In Cellular & molecular immunology, 17, 1014-1016. doi:10.1038/s41423-020-0461-4. https://pubmed.ncbi.nlm.nih.gov/32398807/
3. Hu, Yun-Hong, Zhang, Yu, Jiang, Li-Qun, Shu, Hong-Bing, Liu, Yu. 2015. WDFY1 mediates TLR3/4 signaling by recruiting TRIF. In EMBO reports, 16, 447-55. doi:10.15252/embr.201439637. https://pubmed.ncbi.nlm.nih.gov/25736436/
4. Teranishi, Hirofumi, Tabata, Keisuke, Saeki, Marika, Yoshimori, Tamotsu, Hamasaki, Maho. . Identification of CUL4A-DDB1-WDFY1 as an E3 ubiquitin ligase complex involved in initiation of lysophagy. In Cell reports, 40, 111349. doi:10.1016/j.celrep.2022.111349. https://pubmed.ncbi.nlm.nih.gov/36103833/
5. Zhen, Yuxuan, Shao, Wen-Hai. 2025. WDFY1-expressing follicular dendritic cells play a critical role in lupus development in cGVHD mouse model. In Journal of immunology (Baltimore, Md. : 1950), , . doi:10.1093/jimmun/vkaf017. https://pubmed.ncbi.nlm.nih.gov/40169152/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen
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