C57BL/6JCya-Mrps9em1flox/Cya
Common Name:
Mrps9-flox
Product ID:
S-CKO-14548
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Mrps9-flox
Strain ID
CKOCMP-69527-Mrps9-B6J-VA
Gene Name
Product ID
S-CKO-14548
Gene Alias
2310002A08Rik; Mrp-S9; S9mt; mg637
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
1
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Mrps9em1flox/Cya mice (Catalog S-CKO-14548) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000057208
NCBI RefSeq
NM_023514
Target Region
Exon 3~4
Size of Effective Region
~0.7 kb
Detailed Document
Overview of Gene Research
Mrps9, encoding mitochondrial ribosomal protein S9, is an essential component for the structural and functional integrity of the mitoribosome complex [2]. It plays a pivotal role in regulating mitochondrial function and maintaining structural integrity, and is associated with the PI3K/Akt/mTOR pathway [1].
In ischemic stroke research, bioinformatic analysis revealed significant underexpression of MRPS9 in patients. In vitro experiments with HN cells subjected to oxygen-glucose deprivation/reoxygenation (OGD/R) treatment showed reduced MRPS9 expression, decreased cell viability, and increased apoptosis. Overexpression of MRPS9 mitigated these OGD/R-induced effects. In mouse models, MRPS9 expression was lower in the middle cerebral artery occlusion/reperfusion (MCAO/R) group. KEGG pathway analysis associated MRPS9 with the PI3K/Akt/mTOR pathway, and inhibition of this pathway counteracted the protective effect of MRPS9 overexpression on neuronal apoptosis [1].
In conclusion, Mrps9 is crucial for maintaining mitochondrial function and its regulation of the PI3K/Akt/mTOR pathway has a protective effect on neurons in ischemic stroke. Studies on Mrps9 contribute to understanding the pathophysiology of ischemic stroke and may offer new targets for diagnosis and treatment [1].
References：
1. Jiang, Jina, Qi, Tingting, Li, Li, Ma, Xiaoqing, Qin, Yinghui. 2024. MRPS9-Mediated Regulation of the PI3K/Akt/mTOR Pathway Inhibits Neuron Apoptosis and Protects Ischemic Stroke. In Journal of molecular neuroscience : MN, 74, 23. doi:10.1007/s12031-024-02197-4. https://pubmed.ncbi.nlm.nih.gov/38381220/
2. Cheong, Agnes, Lingutla, Ranjana, Mager, Jesse. 2020. Expression analysis of mammalian mitochondrial ribosomal protein genes. In Gene expression patterns : GEP, 38, 119147. doi:10.1016/j.gep.2020.119147. https://pubmed.ncbi.nlm.nih.gov/32987154/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen