C57BL/6JCya-Spdyaem1flox/Cya
Common Name:
Spdya-flox
Product ID:
S-CKO-14946
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Spdya-flox
Strain ID
CKOCMP-70891-Spdya-B6J-VA
Gene Name
Product ID
S-CKO-14946
Gene Alias
4921517J08Rik; 4930548B21Rik; GS4; MLZ-465; Spdy1
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
17
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Spdyaem1flox/Cya mice (Catalog S-CKO-14946) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000167641
NCBI RefSeq
NM_029254
Target Region
Exon 2
Size of Effective Region
~1.5 kb
Detailed Document
Overview of Gene Research
Spdya, also known as Spy1, is a member of the Speedy/RINGO family. It encodes a protein associated with the cell cycle and meiosis. Spdya can bind to and activate Cdk1 and Cdk2, playing a role in cell proliferation-related pathways [3,4]. Genetic analysis of conditional knockout Spdya mice indicates its essential functions in mammalian reproduction [3].
In meiosis prophase I, the interaction between SUN1 and Spdya is crucial. The SUN1-Spdya-CDK2 ternary complex is formed. Analysis of meiosis in Spdya-binding-deficient SUN1 mutant mice reveals that this interaction is required for telomere-LINC complex connection and the assembly of a ring-shaped telomere supramolecular architecture at the nuclear envelope, which is vital for efficient homologous pairing and synapsis [1]. Additionally, Spdya may promote the telomere-nuclear envelope attachment during meiosis by interacting with SUN1 and recruiting CDK2 [2].
In summary, Spdya plays essential roles in mammalian reproduction, especially in meiotic processes such as telomere-related functions during meiosis prophase I. The study of Spdya-related KO/CKO mouse models has significantly contributed to understanding its functions in reproductive biology [1,2,3].
References：
1. Chen, Yanyan, Wang, Yan, Chen, Juan, Huang, Chenhui, Lei, Ming. 2021. The SUN1-SPDYA interaction plays an essential role in meiosis prophase I. In Nature communications, 12, 3176. doi:10.1038/s41467-021-23550-w. https://pubmed.ncbi.nlm.nih.gov/34039995/
2. Wang, Guishuan, Wu, Xiaolong, Zhou, Liwei, Liang, Ajuan, Sun, Fei. 2020. Tethering of Telomeres to the Nuclear Envelope Is Mediated by SUN1-MAJIN and Possibly Promoted by SPDYA-CDK2 During Meiosis. In Frontiers in cell and developmental biology, 8, 845. doi:10.3389/fcell.2020.00845. https://pubmed.ncbi.nlm.nih.gov/33015044/
3. Hidalgo-Cabrera, A, Bustos, P, Vidal-Pérez, D, Brown, D I, Farlora, R. 2021. Analysis and gonadal localization of Speedy A mRNA transcript, a novel gene associated with early germline cells in the scallop, Argopecten purpuratus. In Animal reproduction science, 236, 106909. doi:10.1016/j.anireprosci.2021.106909. https://pubmed.ncbi.nlm.nih.gov/34954527/
4. Nebreda, Angel R. 2006. CDK activation by non-cyclin proteins. In Current opinion in cell biology, 18, 192-8. doi:. https://pubmed.ncbi.nlm.nih.gov/16488127/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen