C57BL/6JCya-Lsm11em1flox/Cya
Common Name:
Lsm11-flox
Product ID:
S-CKO-15421
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Lsm11-flox
Strain ID
CKOCMP-72290-Lsm11-B6J-VA
Gene Name
Product ID
S-CKO-15421
Gene Alias
2210404M20Rik
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
11
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Lsm11em1flox/Cya mice (Catalog S-CKO-15421) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000129820
NCBI RefSeq
NM_028185
Target Region
Exon 2
Size of Effective Region
~1.1 kb
Detailed Document
Overview of Gene Research
Lsm11, a component of the replication-dependent histone pre-mRNA-processing complex, is crucial for the 3' end processing of metazoan replication-dependent histone pre-mRNAs [1,2,3,4,5,6,7]. U7 snRNP, in which Lsm11 is a part, is distinct from spliceosomal snRNPs as it lacks Sm subunits D1 and D2 and contains Lsm10 and Lsm11 instead [2,3]. Lsm11 is involved in pathways related to histone metabolism and is of great biological importance as histone pre-mRNA processing is essential for proper chromatin structure and cell function [1,2,3,4,5,6,7]. Genetic models, such as Drosophila models, have been valuable for studying Lsm11's function [4,7].
In Drosophila, mutations in Lsm11 disrupt normal histone pre-mRNA processing, leading to the production of poly(A)+ histone mRNA due to transcriptional read-through to cryptic polyadenylation sites [7]. A two-amino-acid mutation of Drosophila Lsm11 that prevents its binding to FLASH (a processing factor that binds Lsm11) cannot rescue the lethality or histone pre-mRNA processing defects resulting from an Lsm11 null mutation, indicating the importance of this interaction for histone pre-mRNA processing in vivo and for proper development and viability in flies [4]. In human, biallelic mutations in Lsm11 are associated with Aicardi-Goutières syndrome, a rare monogenic autoimmune disease. These mutations lead to misprocessing of canonical histone transcripts, disturbance of linker histone stoichiometry, altered distribution of nuclear cyclic guanosine monophosphate-adenosine monophosphate synthase (cGAS), and enhanced interferon signaling mediated by the cGAS-stimulator of interferon genes (STING) pathway [1].
In conclusion, Lsm11 is essential for histone pre-mRNA processing, which is vital for maintaining proper chromatin structure and normal cellular function. Model-based research, especially in Drosophila, has revealed its role in development and viability. In humans, its malfunction due to mutations is associated with Aicardi-Goutières syndrome, highlighting its significance in understanding this autoimmune disease.
References：
1. Uggenti, Carolina, Lepelley, Alice, Depp, Marine, Gilbert, Nick, Crow, Yanick J. 2020. cGAS-mediated induction of type I interferon due to inborn errors of histone pre-mRNA processing. In Nature genetics, 52, 1364-1372. doi:10.1038/s41588-020-00737-3. https://pubmed.ncbi.nlm.nih.gov/33230297/
2. Yang, Xiao-Cui, Desotell, Anthony, Lin, Min-Han, Tong, Liang, Dominski, Zbigniew. 2023. In vitro methylation of the U7 snRNP subunits Lsm11 and SmE by the PRMT5/MEP50/pICln methylosome. In RNA (New York, N.Y.), 29, 1673-1690. doi:10.1261/rna.079709.123. https://pubmed.ncbi.nlm.nih.gov/37562960/
3. Yang, Xiao-Cui, Desotell, Anthony, Lin, Min-Han, Tong, Liang, Dominski, Zbigniew. 2023. In vitro methylation of the U7 snRNP subunits Lsm11 and SmE by the PRMT5/MEP50/pICln methylosome. In bioRxiv : the preprint server for biology, , . doi:10.1101/2023.05.10.540203. https://pubmed.ncbi.nlm.nih.gov/37215023/
4. Burch, Brandon D, Godfrey, Ashley C, Gasdaska, Pamela Y, Marzluff, William F, Dominski, Zbigniew. 2011. Interaction between FLASH and Lsm11 is essential for histone pre-mRNA processing in vivo in Drosophila. In RNA (New York, N.Y.), 17, 1132-47. doi:10.1261/rna.2566811. https://pubmed.ncbi.nlm.nih.gov/21525146/
5. Azzouz, Teldja N, Gruber, Andreas, Schümperli, Daniel. 2005. U7 snRNP-specific Lsm11 protein: dual binding contacts with the 100 kDa zinc finger processing factor (ZFP100) and a ZFP100-independent function in histone RNA 3' end processing. In Nucleic acids research, 33, 2106-17. doi:. https://pubmed.ncbi.nlm.nih.gov/15824063/
6. Aik, Wei Shen, Lin, Min-Han, Tan, Dazhi, Chou, Chi-Yuan, Tong, Liang. 2017. The N-terminal domains of FLASH and Lsm11 form a 2:1 heterotrimer for histone pre-mRNA 3'-end processing. In PloS one, 12, e0186034. doi:10.1371/journal.pone.0186034. https://pubmed.ncbi.nlm.nih.gov/29020104/
7. Godfrey, Ashley C, White, Anne E, Tatomer, Deirdre C, Marzluff, William F, Duronio, Robert J. 2009. The Drosophila U7 snRNP proteins Lsm10 and Lsm11 are required for histone pre-mRNA processing and play an essential role in development. In RNA (New York, N.Y.), 15, 1661-72. doi:10.1261/rna.1518009. https://pubmed.ncbi.nlm.nih.gov/19620235/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen