C57BL/6JCya-Cyp2j9em1flox/Cya
Common Name:
Cyp2j9-flox
Product ID:
S-CKO-16040
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
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Basic Information
Strain Name
Cyp2j9-flox
Strain ID
CKOCMP-74519-Cyp2j9-B6J-VA
Gene Name
Product ID
S-CKO-16040
Gene Alias
8430417E17Rik
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
4
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Cyp2j9em1flox/Cya mice (Catalog S-CKO-16040) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000055693
NCBI RefSeq
NM_028979
Target Region
Exon 2
Size of Effective Region
~0.7 kb
Detailed Document
Overview of Gene Research
Cyp2j9, a member of the cytochrome P450 CYP2J subfamily, is an arachidonic acid omega-1 hydroxylase. It metabolizes arachidonic acid to 19-hydroxyeicosatetraenoic acid (19-HETE) [2]. This gene is highly expressed in the brain, liver, and lung, and is also expressed in mouse atrial HL-1 cells at a similar level to that in mouse hearts [2,4]. In the brain, it may play important functional roles as its product 19-HETE can inhibit the activity of P/Q-type Ca(2+) channels expressed in cerebellar Purkinje cells [2].
In lipopolysaccharide (LPS)-induced acute lung injury (ALI) in mice, the mRNA expression of Cyp2j9 was decreased [1]. This dysregulation of Cyp2j9, along with other CYPs/COX-2 metabolism changes of arachidonic acid, contributed to the uncontrolled inflammatory response in ALI [1]. In C57BL/6 mice, arsenic trioxide (ATO) induced Cyp2j9, which led to changes in the production of hydroxyeicosatetraenoic acids (HETEs), suggesting that ATO-induced hepatotoxicity may be related to the modification of bioactive AA metabolites by modulating Cyp2j9 and other CYPs [3].
In conclusion, Cyp2j9 plays a role in arachidonic acid metabolism and is involved in processes related to inflammation and potential toxicity. Studies in mouse models, such as those with LPS-induced ALI and ATO treatment, have revealed its significance in these disease-related conditions, providing insights into how its dysregulation can contribute to pathophysiological processes.
References:
1. Yang, Hui-Hui, Duan, Jia-Xi, Liu, Shao-Kun, Zhou, Yong, Guan, Cha-Xiang. 2020. A COX-2/sEH dual inhibitor PTUPB alleviates lipopolysaccharide-induced acute lung injury in mice by inhibiting NLRP3 inflammasome activation. In Theranostics, 10, 4749-4761. doi:10.7150/thno.43108. https://pubmed.ncbi.nlm.nih.gov/32308747/
2. Qu, W, Bradbury, J A, Tsao, C C, Rosenberg, R L, Zeldin, D C. 2001. Cytochrome P450 CYP2J9, a new mouse arachidonic acid omega-1 hydroxylase predominantly expressed in brain. In The Journal of biological chemistry, 276, 25467-79. doi:. https://pubmed.ncbi.nlm.nih.gov/11328810/
3. El-Ghiaty, Mahmoud A, Alqahtani, Mohammed A, El-Mahrouk, Sara R, Alammari, Ahmad H, El-Kadi, Ayman O S. 2024. Alteration of Hepatic Cytochrome P450 Expression and Arachidonic Acid Metabolism by Arsenic Trioxide (ATO) in C57BL/6 Mice. In Biological trace element research, 203, 1000-1015. doi:10.1007/s12011-024-04225-1. https://pubmed.ncbi.nlm.nih.gov/38758479/
4. Elshenawy, Osama H, Anwar-Mohamed, Anwar, Abdelhamid, Ghada, El-Kadi, Ayman O S. 2012. Murine atrial HL-1 cell line is a reliable model to study drug metabolizing enzymes in the heart. In Vascular pharmacology, 58, 326-33. doi:10.1016/j.vph.2012.12.002. https://pubmed.ncbi.nlm.nih.gov/23268359/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen