C57BL/6JCya-Meiobem1flox/Cya
Common Name:
Meiob-flox
Product ID:
S-CKO-16170
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
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Basic Information
Strain Name
Meiob-flox
Strain ID
CKOCMP-75178-Meiob-B6J-VA
Gene Name
Product ID
S-CKO-16170
Gene Alias
4930528F23Rik; MLZ-675
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
17
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Meiobem1flox/Cya mice (Catalog S-CKO-16170) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000024972
NCBI RefSeq
NM_029197
Target Region
Exon 7~10
Size of Effective Region
~5.6 kb
Detailed Document
Overview of Gene Research
Meiob, also known as meiosis specific with OB domain, is a crucial gene for meiotic recombination. It codes for a single-strand DNA binding protein that participates in DNA double-strand breaks repair during meiosis [3]. Meiob forms a complex with RPA and SPATA22, and these proteins co-localize on meiotic chromosomes. The protein contains an OB domain similar to one of the RPA1 OB folds and exhibits 3'-5' exonuclease activity [4].
In Meiob-null mouse mutants, both male and female mice are infertile due to meiotic arrest at a zygotene/pachytene-like stage. DNA double-strand break repair and homologous chromosome synapsis are impaired in these meiocytes [5]. In humans, novel variants in the MEIOB gene have been identified in patients with primary ovarian insufficiency (POI) and non-obstructive azoospermia (NOA). These variants, such as frameshift, nonsense, and non-canonical splicing variants, produce truncated proteins, affect protein function, and lead to meiotic arrest [1,2].
In conclusion, Meiob is essential for meiotic recombination and chromosomal synapsis. Studies on Meiob-null mouse models and human patients with MEIOB variants have revealed its crucial role in fertility-related diseases like POI and NOA. Understanding the function of Meiob provides insights into the genetic basis of infertility, which is valuable for genetic counseling and patient management.
References：
1. Wang, Yurong, Liu, Ling, Tan, Chen, He, Wen-Bin, Tan, Yue-Qiu. 2022. Novel MEIOB variants cause primary ovarian insufficiency and non-obstructive azoospermia. In Frontiers in genetics, 13, 936264. doi:10.3389/fgene.2022.936264. https://pubmed.ncbi.nlm.nih.gov/35991565/
2. Zhu, Xiaoyu, Hu, Kaiqin, Cheng, Huiru, Tang, Dongdong, Guo, Rui. 2023. Novel MEIOB pathogenic variants including a homozygous non-canonical splicing variant, cause meiotic arrest and human non-obstructive azoospermia. In Clinical genetics, 105, 99-105. doi:10.1111/cge.14426. https://pubmed.ncbi.nlm.nih.gov/37715646/
3. Gershoni, Moran, Hauser, Ron, Barda, Shimi, Pietrokovski, Shmuel, Kleiman, Sandra E. . A new MEIOB mutation is a recurrent cause for azoospermia and testicular meiotic arrest. In Human reproduction (Oxford, England), 34, 666-671. doi:10.1093/humrep/dez016. https://pubmed.ncbi.nlm.nih.gov/30838384/
4. Luo, Mengcheng, Yang, Fang, Leu, N Adrian, La Salle, Sophie, Wang, P Jeremy. . MEIOB exhibits single-stranded DNA-binding and exonuclease activities and is essential for meiotic recombination. In Nature communications, 4, 2788. doi:10.1038/ncomms3788. https://pubmed.ncbi.nlm.nih.gov/24240703/
5. Souquet, Benoit, Abby, Emilie, Hervé, Roxane, Habert, René, Livera, Gabriel. 2013. MEIOB targets single-strand DNA and is necessary for meiotic recombination. In PLoS genetics, 9, e1003784. doi:10.1371/journal.pgen.1003784. https://pubmed.ncbi.nlm.nih.gov/24068956/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen