C57BL/6NCya-Zcchc4em1flox/Cya
Common Name
Zcchc4-flox
Product ID
S-CKO-16867
Backgroud
C57BL/6NCya
Strain ID
CKOCMP-78796-Zcchc4-B6N-VA
Status
When using this mouse strain in a publication, please cite “Zcchc4-flox Mouse (Catalog S-CKO-16867) were purchased from Cyagen.”
Product Type
Age
Genotype
Sex
Quantity
The standard delivery applies for a guaranteed minimum of three heterozygous carriers. Breeding services for homozygous carriers and/or specified sex are available.
Basic Information
Strain Name
Zcchc4-flox
Strain ID
CKOCMP-78796-Zcchc4-B6N-VA
Gene Name
Product ID
S-CKO-16867
Gene Alias
4930449I23Rik
Background
C57BL/6NCya
Gene Full Name
zinc finger, CCHC domain containing 4
Modification
Conditional knockout
NCBI ID
Phenotype
Chromosome
Chr 5 (Mouse)
Application
--
Datasheet
Strain Description
Ensembl Transcript ID
ENSMUST00000031077
NCBI Transcript ID
NM_030185
Target Region
Exon 2~3
Size of Effective Region
~2.6 kb
Overview of Gene Research
Zcchc4, zinc finger CCHC domain-containing protein 4, is an N6 -methyladenosine (m6A) RNA methyltransferase. It mainly methylates human 28S rRNA, and this rRNA m6A modification is significant for translation [4,5,6]. Zcchc4 also has an impact on various biological processes related to cancer, such as affecting apoptosis and chemoresistance pathways [1].
In cancer research, knockout of Zcchc4 in HCC cells reduces tumor growth in vivo and enhances the antitumor effect of DNA -damage agents, as it promotes the interaction between a lncRNA AL133467.2 and γH2AX to enhance chemosensitivity [1]. In osteosarcoma, knockdown of Zcchc4 in an animal model reduces tumor growth and metastases, as it normally promotes the disease progression by upregulating ITGB1 [2]. In esophageal cancer, down -regulation of Zcchc4 in vivo inhibits cancer progression and reduces cisplatin resistance, as it leads to increased ROS, S -phase arrest, and enhanced apoptosis [3].
In summary, Zcchc4 is crucial for rRNA methylation and translation. Its knockout or down -regulation in various cancer models reveals its promoting role in cancer progression and chemoresistance. These findings suggest Zcchc4 as a potential target for improving cancer chemotherapy, contributing to a better understanding of cancer -related biological processes [1,2,3].
References：
1. Zhu, Ha, Chen, Kun, Chen, Yali, Chen, Taoyong, Cao, Xuetao. 2022. RNA-binding protein ZCCHC4 promotes human cancer chemoresistance by disrupting DNA-damage-induced apoptosis. In Signal transduction and targeted therapy, 7, 240. doi:10.1038/s41392-022-01033-8. https://pubmed.ncbi.nlm.nih.gov/35853866/
2. Luo, Lingli, Tang, Xiaojun, Liu, Linghua, Chang, Guiping, Xiao, Zhihong. . ZCCHC4 Promotes Osteosarcoma Progression by Upregulating ITGB1. In Critical reviews in eukaryotic gene expression, 33, 31-39. doi:10.1615/CritRevEukaryotGeneExpr.2023047798. https://pubmed.ncbi.nlm.nih.gov/37606162/
3. Yao, Lihua, Wu, Piao, Yao, Fangyi, Zhong, Fangmin, Wang, Xiaozhong. 2025. ZCCHC4 regulates esophageal cancer progression and cisplatin resistance through ROS/c-myc axis. In Scientific reports, 15, 5149. doi:10.1038/s41598-025-89628-3. https://pubmed.ncbi.nlm.nih.gov/39934309/
4. Ma, Honghui, Wang, Xiaoyun, Cai, Jiabin, Shi, Yang, He, Chuan. 2018. N6-Methyladenosine methyltransferase ZCCHC4 mediates ribosomal RNA methylation. In Nature chemical biology, 15, 88-94. doi:10.1038/s41589-018-0184-3. https://pubmed.ncbi.nlm.nih.gov/30531910/
5. Ren, Wendan, Lu, Jiuwei, Huang, Mengjiang, Wang, Gang Greg, Song, Jikui. 2019. Structure and regulation of ZCCHC4 in m6A-methylation of 28S rRNA. In Nature communications, 10, 5042. doi:10.1038/s41467-019-12923-x. https://pubmed.ncbi.nlm.nih.gov/31695039/
6. Pinto, Rita, Vågbø, Cathrine B, Jakobsson, Magnus E, Slupphaug, Geir, Falnes, Pål Ø. . The human methyltransferase ZCCHC4 catalyses N6-methyladenosine modification of 28S ribosomal RNA. In Nucleic acids research, 48, 830-846. doi:10.1093/nar/gkz1147. https://pubmed.ncbi.nlm.nih.gov/31799605/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen
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