C57BL/6JCya-Cptpem1flox/Cya
Common Name:
Cptp-flox
Product ID:
S-CKO-16922
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
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Basic Information
Strain Name
Cptp-flox
Strain ID
CKOCMP-79554-Cptp-B6J-VA
Gene Name
Product ID
S-CKO-16922
Gene Alias
Gltpd1
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
4
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Cptpem1flox/Cya mice (Catalog S-CKO-16922) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000030950
NCBI RefSeq
NM_024472
Target Region
Exon 2~3
Size of Effective Region
~1.3 kb
Detailed Document
Overview of Gene Research
Cptp, short for ceramide-1-phosphate transfer protein, is a member of the glycolipid transfer protein family. It shuttles ceramide-1-phosphate (C1P) between membranes, regulating cellular sphingolipid homeostasis. This function impacts programmed cell death, inflammation, autophagy and inflammasome activation. It is also associated with pathways such as the PI4KA/AKT signaling pathway [1,2,3,4].
In pancreatic cancer cells, high expression of Cptp is linked to poor prognosis. Knockdown of Cptp in human epithelial cells stimulates autophagosome formation and alters autophagy-related protein expression levels. In THP-1 macrophage-like cells, Cptp knockdown not only induces autophagy but also elevates caspase-1 levels and increases IL-1β and IL-18 release via an NLRP3 inflammasome-based mechanism [1,3].
In conclusion, Cptp is an endogenous regulator of early-stage autophagosome assembly and inflammasome-driven pro-inflammatory cytokine generation. Its role in pancreatic cancer and in regulating autophagy and inflammation suggests that it could be a potential therapeutic target in these disease areas [1,3].
References：
1. Zhang, Yanqun, Ji, Shenying, Zhang, Xiangyu, Lam, Sin Man, Zou, Xianqiong. 2022. Human CPTP promotes growth and metastasis via sphingolipid metabolite ceramide and PI4KA/AKT signaling in pancreatic cancer cells. In International journal of biological sciences, 18, 4963-4983. doi:10.7150/ijbs.70007. https://pubmed.ncbi.nlm.nih.gov/35982909/
2. Gao, Yong-Guang, Zhai, Xiuhong, Boldyrev, Ivan A, Malinina, Lucy, Brown, Rhoderick E. 2021. Ceramide-1-phosphate transfer protein (CPTP) regulation by phosphoinositides. In The Journal of biological chemistry, 296, 100600. doi:10.1016/j.jbc.2021.100600. https://pubmed.ncbi.nlm.nih.gov/33781749/
3. Mishra, Shrawan Kumar, Gao, Yong-Guang, Deng, Yibin, Hinchcliffe, Edward H, Brown, Rhoderick E. 2018. CPTP: A sphingolipid transfer protein that regulates autophagy and inflammasome activation. In Autophagy, 14, 862-879. doi:10.1080/15548627.2017.1393129. https://pubmed.ncbi.nlm.nih.gov/29164996/
4. Zhang, Yanqun, Zhang, Xiangyu, Lu, Mengyun, Zou, Xianqiong. 2021. Ceramide-1-phosphate and its transfer proteins in eukaryotes. In Chemistry and physics of lipids, 240, 105135. doi:10.1016/j.chemphyslip.2021.105135. https://pubmed.ncbi.nlm.nih.gov/34499882/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen