C57BL/6JCya-Glod4em1flox/Cya
Common Name
Glod4-flox
Product ID
S-CKO-17641
Backgroud
C57BL/6JCya
Strain ID
CKOCMP-67201-Glod4-B6J-VC
When using this mouse strain in a publication, please cite “Glod4-flox Mouse (Catalog S-CKO-17641) were purchased from Cyagen.”
Product Type
Age
Genotype
Sex
Quantity
Basic Information
Strain Name
Glod4-flox
Strain ID
CKOCMP-67201-Glod4-B6J-VC
Gene Name
Product ID
S-CKO-17641
Gene Alias
1700082G03Rik, 2700085E05Rik
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
Chr 11
Phenotype
Datasheet
Application
--
Strain Description
Ensembl Number
ENSMUST00000017430
NCBI RefSeq
NM_026029.3
Target Region
Exon 3~4
Size of Effective Region
~2.4 kb
Overview of Gene Research
GLOD4, glyoxalase domain containing protein 4, belongs to the glyoxalase gene family, yet its physiological role remains relatively unknown compared to some family members [4]. The common feature of this family is the presence of structural motifs coordinating divalent cations for enzyme activity, with functions related to metabolism and aldehyde detoxication [4].
In Alzheimer's disease (AD) research, GLOD4 mRNA and protein isoforms were downregulated in cortical tissues from AD patients [1]. In Blmh -/- 5xFAD mouse AD models, Glod4 mRNA was downregulated, associated with elevated Aβ and worsened memory/sensorimotor performance [1]. In N2a-APPswe cells, Glod4 depletion upregulated AβPP and downregulated autophagy-related genes, suggesting GLOD4 interacts with AβPP and the autophagy pathway [1].
In the study of testicular development, in Tibetan sheep, GLOD4 expressions at both mRNA and protein levels were significantly higher in 1-year-old and 3-year-old testes compared to 3-month-old ones, and the protein was mainly localized in the cytoplasm of Leydig cells, indicating its possible role in Leydig cell development [2]. In Qianbei Ma goats, GLOD4 was mainly expressed in Leydig cells. Silencing GLOD4 suppressed testosterone secretion-related and cell cycle-related genes, blocked the cell cycle at G2/M phase, and reduced testosterone secretion, while overexpression had the opposite effects, suggesting its influence on cell development via cell cycle, proliferation, and testosterone synthesis [3].
In Caenorhabditis elegans, glod-4 mutation led to increased food intake, and RNAseq analysis identified upregulation of several neurotransmitters and feeding genes [5].
In conclusion, through studies in models like mice and nematodes, GLOD4 has been shown to be involved in processes such as Alzheimer's disease-related Aβ regulation and autophagy, as well as testicular cell development and feeding behavior in nematodes. These model-based studies have provided valuable insights into the role of GLOD4 in specific biological processes and disease-related conditions [1,2,3,5].
References：
1. Utyro, Olga, Włoczkowska-Łapińska, Olga, Jakubowski, Hieronim. . Association of GLOD4 with Alzheimer's Disease in Humans and Mice. In Journal of Alzheimer's disease : JAD, 101, 823-834. doi:10.3233/JAD-240512. https://pubmed.ncbi.nlm.nih.gov/39302370/
2. Wang, Xia, Li, Taotao, Liu, Ningbo, Zhao, Xingxu, Ma, Youji. 2019. Characterization of GLOD4 in Leydig Cells of Tibetan Sheep During Different Stages of Maturity. In Genes, 10, . doi:10.3390/genes10100796. https://pubmed.ncbi.nlm.nih.gov/31614839/
3. Wang, Jinqian, Chen, Xiang, Sun, Wei, Li, Ruiyang, Wang, Yanfei. 2024. Expression of GLOD4 in the Testis of the Qianbei Ma Goat and Its Effect on Leydig Cells. In Animals : an open access journal from MDPI, 14, . doi:10.3390/ani14172611. https://pubmed.ncbi.nlm.nih.gov/39272396/
4. Farrera, Dominique O, Galligan, James J. 2022. The Human Glyoxalase Gene Family in Health and Disease. In Chemical research in toxicology, 35, 1766-1776. doi:10.1021/acs.chemrestox.2c00182. https://pubmed.ncbi.nlm.nih.gov/36048613/
5. Muthaiyan Shanmugam, Muniesh, Chaudhuri, Jyotiska, Sellegounder, Durai, Galligan, James J, Kapahi, Pankaj. 2023. Methylglyoxal-derived hydroimidazolone, MG-H1, increases food intake by altering tyramine signaling via the GATA transcription factor ELT-3 in Caenorhabditis elegans. In eLife, 12, . doi:10.7554/eLife.82446. https://pubmed.ncbi.nlm.nih.gov/37728328/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen
Contact Us
Connect with our experts for your custom animal model needs. Please fill out the form below to start a conversation or request a quote.
Cyagen values your privacy. We’d like to keep you informed about our latest offerings and insights. Your preferences:
You may unsubscribe from these communications at any time. See our Privacy Policy for details on opting out and data protection.
By clicking the button below, you consent to allow Cyagen to store and process the personal information submitted in this form to provide you the content requested.