C57BL/6JCya-Mir382em1flox/Cya
Common Name:
Mir382-flox
Product ID:
S-CKO-17666
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
Price:
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Basic Information
Strain Name
Mir382-flox
Strain ID
CKOCMP-723912-Mir382-B6J-VB
Gene Name
Product ID
S-CKO-17666
Gene Alias
Mirn382; mir-382; mmu-mir-382
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
12
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Mir382em1flox/Cya mice (Catalog S-CKO-17666) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000083494
NCBI RefSeq
NR_029883
Target Region
Exon 1
Size of Effective Region
~1.1 kb
Detailed Document
Overview of Gene Research
Mir382 is a microRNA that has been shown to play regulatory roles in various physiological processes such as osteogenic differentiation, hematopoietic stem cell differentiation, normal hematopoiesis, and liver progenitor cell differentiation [4]. It is also involved in multiple signaling pathways, for example, it was found to target the PTEN-Akt axis, regulating hepatocyte proliferation and cell growth during liver regeneration [7].
Deregulation of Mir382 has been widely reported in different cancers. In many oncogenesis studies, Mir382 downregulation was observed in cancers, and it exerted a suppressor impact on the malignant phenotype of cancer cells both in vitro and in vivo [4]. In urogenital cancers, curcumin exerts its anticancer effects in part by downregulating oncogenic microRNAs including Mir382 [3]. Additionally, Mir382 is among the miRNAs that interact with DSCAM-AS1, an lncRNA with pathogenic roles in multiple cancers [1].
In colorectal cancer, Mir382 is overexpressed in cancer-associated fibroblasts (CAFs), and these miRNAs can influence the spread, invasiveness, and chemoresistance in neighboring tumor cells via paracrine signaling [2]. It is also involved in the development of osteosarcoma stem cells, which are associated with tumor initiation, metastasis, recurrence, and drug resistance [6]. In papillary thyroid cancer, the expression level of Mir382-5p was found to be different in patients compared to healthy controls [8]. Moreover, in high-fat-diet-induced obese mice, liraglutide treatment decreased the expression of Mir382, which was negatively related to the browning of white adipose tissue [5].
In conclusion, Mir382 plays a crucial role in various biological processes and diseases, especially in cancer. Its deregulation is associated with different cancer types, and its involvement in multiple cellular and signaling pathways makes it a potential diagnostic and therapeutic marker. Studies on Mir382, including those using in vivo models, contribute to a better understanding of its functions and its potential in cancer treatment [1,2,3,4,5,6,7,8].
References：
1. Ghafouri-Fard, Soudeh, Khoshbakht, Tayyebeh, Taheri, Mohammad, Ebrahimzadeh, Kaveh. 2021. A Review on the Carcinogenic Roles of DSCAM-AS1. In Frontiers in cell and developmental biology, 9, 758513. doi:10.3389/fcell.2021.758513. https://pubmed.ncbi.nlm.nih.gov/34708048/
2. Savardashtaki, Amir, Shabaninejad, Zahra, Movahedpour, Ahmad, Mirzaei, Hamed, Hamblin, Michael R. 2019. miRNAs derived from cancer-associated fibroblasts in colorectal cancer. In Epigenomics, 11, 1627-1645. doi:10.2217/epi-2019-0110. https://pubmed.ncbi.nlm.nih.gov/31702390/
3. Mazaheri-Tehrani, Sadegh, Rouzbahani, Shiva, Heidari-Beni, Motahar. 2024. The Association Between Anti-Neoplastic Effects of Curcumin and Urogenital Cancers: A Systematic Review. In BioMed research international, 2024, 9347381. doi:10.1155/2024/9347381. https://pubmed.ncbi.nlm.nih.gov/39445208/
4. Fattahi, Mehdi, Shahrabi, Saeid, Saadatpour, Fatemeh, Najafi, Sajad, Le, Binh Nguyen. 2023. microRNA-382 as a tumor suppressor? Roles in tumorigenesis and clinical significance. In International journal of biological macromolecules, 250, 125863. doi:10.1016/j.ijbiomac.2023.125863. https://pubmed.ncbi.nlm.nih.gov/37467828/
5. Zhao, Li, Li, Wenxin, Zhang, Panpan, Yang, Ling, Yuan, Guoyue. 2024. Liraglutide induced browning of visceral white adipose through regulation of miRNAs in high-fat-diet-induced obese mice. In Endocrine, 85, 222-232. doi:10.1007/s12020-024-03734-2. https://pubmed.ncbi.nlm.nih.gov/38378894/
6. Liu, Jinxin, Shang, Guanning. 2022. The Roles of Noncoding RNAs in the Development of Osteosarcoma Stem Cells and Potential Therapeutic Targets. In Frontiers in cell and developmental biology, 10, 773038. doi:10.3389/fcell.2022.773038. https://pubmed.ncbi.nlm.nih.gov/35252166/
7. Bei, Yihua, Song, Yang, Wang, Fei, Xiao, Junjie, Yang, Changqing. . miR-382 targeting PTEN-Akt axis promotes liver regeneration. In Oncotarget, 7, 1584-97. doi:10.18632/oncotarget.6444. https://pubmed.ncbi.nlm.nih.gov/26636539/
8. Capriglione, Francesca, Verrienti, Antonella, Celano, Marilena, Bulotta, Stefania, Russo, Diego. 2021. Analysis of serum microRNA in exosomal vehicles of papillary thyroid cancer. In Endocrine, 75, 185-193. doi:10.1007/s12020-021-02847-2. https://pubmed.ncbi.nlm.nih.gov/34378123/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen