C57BL/6JCya-Haspinem1flox/Cya
Common Name:
Haspin-flox
Product ID:
S-CKO-17752
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
Price:
Contact for Pricing
Basic Information
Strain Name
Haspin-flox
Strain ID
CKOCMP-14841-Haspin-B6J-VB
Gene Name
Product ID
S-CKO-17752
Gene Alias
Gsg2
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
11
Phenotype
Document
Application
--
Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Haspinem1flox/Cya mice (Catalog S-CKO-17752) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000052140
NCBI RefSeq
NM_010353
Target Region
Exon 1
Size of Effective Region
~3.2 kb
Detailed Document
Overview of Gene Research
Haspin, also known as haploid germ cell-specific nuclear protein kinase, is an atypical serine/threonine kinase. It lacks sequence similarity with other eukaryotic protein kinases. Haspin is crucial for cell cycle progression, especially in mitosis, where it phosphorylates threonine 3 of histone 3 (H3T3), which recruits the chromosomal passenger complex to the inner centromere and ensures proper chromosome alignment and segregation. It also seems to have a role in post-meiotic spermatogenesis as it is highly expressed in the testis [1,2,3].
Depletion of Haspin by RNA interference in human cell lines causes premature loss of centromeric cohesin from chromosomes in mitosis, failure of metaphase chromosome alignment, and subsequent mitotic arrest [3]. In gallbladder carcinoma, knockdown of HASPIN inhibited cell proliferation, clone-formation ability, and promoted apoptosis both in vitro and in vivo, indicating its role in promoting cancer progression [4]. Inhibiting Haspin using small molecule inhibitors like CHR-6494 or 5-ITu can delay mitotic entry at the G1/S or S/G2 boundaries, suggesting its role in regulating cell cycle progression at multiple stages [5].
In conclusion, Haspin is essential for normal mitosis and cell cycle regulation. Its overexpression or deletion leads to defective mitosis. The study of Haspin through loss-of-function experiments, such as knockdown in cell lines, has revealed its significance in cancer development, making it a promising target for anticancer drug design.
References:
1. Amoussou, Nathalie Gisèle, Bigot, André, Roussakis, Christos, Robert, Jean-Michel H. 2017. Haspin: a promising target for the design of inhibitors as potent anticancer drugs. In Drug discovery today, 23, 409-415. doi:10.1016/j.drudis.2017.10.005. https://pubmed.ncbi.nlm.nih.gov/29031622/
2. Liu, Yongjian, Yang, Hongliu, Fang, Yongsheng, Zhang, Yuanyuan, Liu, Yonggang. . Function and inhibition of Haspin kinase: targeting multiple cancer therapies by antimitosis. In The Journal of pharmacy and pharmacology, 75, 445-465. doi:10.1093/jpp/rgac080. https://pubmed.ncbi.nlm.nih.gov/36334086/
3. Higgins, Jonathan M G. 2009. Haspin: a newly discovered regulator of mitotic chromosome behavior. In Chromosoma, 119, 137-47. doi:10.1007/s00412-009-0250-4. https://pubmed.ncbi.nlm.nih.gov/19997740/
4. Zhu, Dawei, Gu, Xing, Lin, Zhenyu, Wang, Jing, Li, Li. 2020. HASPIN is involved in the progression of gallbladder carcinoma. In Experimental cell research, 390, 111863. doi:10.1016/j.yexcr.2020.111863. https://pubmed.ncbi.nlm.nih.gov/31987787/
5. Wang, Peiling, Hua, Xiangmei, Bryner, Yuge Han, Gitter, Christopher B, Dai, Jun. 2019. Haspin inhibition delays cell cycle progression through interphase in cancer cells. In Journal of cellular physiology, 235, 4508-4519. doi:10.1002/jcp.29328. https://pubmed.ncbi.nlm.nih.gov/31625162/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen