C57BL/6JCya-Clppem1flox/Cya
Common Name:
Clpp-flox
Product ID:
S-CKO-17761
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
Price:
Contact for Pricing
Basic Information
Strain Name
Clpp-flox
Strain ID
CKOCMP-53895-Clpp-B6J-VB
Gene Name
Product ID
S-CKO-17761
Gene Alias
D17Wsu160e
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
17
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Clppem1flox/Cya mice (Catalog S-CKO-17761) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000002735
NCBI RefSeq
NM_017393
Target Region
Exon 3~5
Size of Effective Region
~3.2 kb
Detailed Document
Overview of Gene Research
ClpP, the caseinolytic protease P, is a serine protease crucial for proteostasis in eukaryotic organelles and prokaryotic cells [3]. In mitochondria, it plays a central role in protein quality control by degrading misfolded or damaged proteins, thus maintaining normal metabolic function [1,2,6,7]. It forms a multimeric complex with the ATP-dependent unfoldase ClpX (ClpXP) to exhibit proteolytic activity [6]. Its substrates are involved in essential mitochondrial processes like the Krebs cycle, oxidative phosphorylation, and fatty acid metabolism [4].
In a conditional knockout (cKO) mouse model, ClpP/ClpX deficiency was found to impair mitochondrial functions and mTORC1 signaling during spermatogenesis. The deficiency reduced mitochondrial functions and quantity in spermatocytes, affected energy supply during meiosis, and attenuated zygotene-pachytene transformation of male germ cells, eventually leading to apoptosis and decreased testicular size [5]. In another study, reduced ClpP expression in a diet-induced non-alcoholic steatohepatitis (NASH) mouse model led to mitochondrial dysfunction, which was a key factor in NASH development. Overexpression or activation of ClpP reversed mitochondrial dysfunction and improved NASH characteristics [2]. Also, hyperactivation of ClpP selectively killed cancer cells by degrading respiratory chain protein substrates and disrupting mitochondrial structure and function, independent of p53 status [1].
In conclusion, ClpP is essential for maintaining mitochondrial protein quality control and normal metabolic function. The ClpP/ClpX cKO mouse models have revealed its critical roles in spermatogenesis, while studies on diet-induced NASH models and cancer cell lines have shown its potential as a therapeutic target in NASH and cancer [1,2,5].
References:
1. Ishizawa, Jo, Zarabi, Sarah F, Davis, R Eric, Schimmer, Aaron D, Andreeff, Michael. 2019. Mitochondrial ClpP-Mediated Proteolysis Induces Selective Cancer Cell Lethality. In Cancer cell, 35, 721-737.e9. doi:10.1016/j.ccell.2019.03.014. https://pubmed.ncbi.nlm.nih.gov/31056398/
2. Choi, Sung-E, Hwang, Yoonjung, Lee, Soo-Jin, Song, Hyun Kyu, Kang, Yup. 2022. Mitochondrial protease ClpP supplementation ameliorates diet-induced NASH in mice. In Journal of hepatology, 77, 735-747. doi:10.1016/j.jhep.2022.03.034. https://pubmed.ncbi.nlm.nih.gov/35421426/
3. Moreno-Cinos, Carlos, Goossens, Kenneth, Salado, Irene G, De Winter, Hans, Augustyns, Koen. 2019. ClpP Protease, a Promising Antimicrobial Target. In International journal of molecular sciences, 20, . doi:10.3390/ijms20092232. https://pubmed.ncbi.nlm.nih.gov/31067645/
4. Mabanglo, Mark F, Bhandari, Vaibhav, Houry, Walid A. 2021. Substrates and interactors of the ClpP protease in the mitochondria. In Current opinion in chemical biology, 66, 102078. doi:10.1016/j.cbpa.2021.07.003. https://pubmed.ncbi.nlm.nih.gov/34446368/
5. Guo, Chenxi, Xiao, Yuan, Gu, Jingkai, Yeung, William S B, Wang, Tianren. 2023. ClpP/ClpX deficiency impairs mitochondrial functions and mTORC1 signaling during spermatogenesis. In Communications biology, 6, 1012. doi:10.1038/s42003-023-05372-2. https://pubmed.ncbi.nlm.nih.gov/37798322/
6. Nouri, Kazem, Feng, Yue, Schimmer, Aaron D. 2020. Mitochondrial ClpP serine protease-biological function and emerging target for cancer therapy. In Cell death & disease, 11, 841. doi:10.1038/s41419-020-03062-z. https://pubmed.ncbi.nlm.nih.gov/33037181/
7. Luo, Baozhu, Ma, Yu, Zhou, YuanZheng, Zhang, Nannan, Luo, Youfu. 2021. Human ClpP protease, a promising therapy target for diseases of mitochondrial dysfunction. In Drug discovery today, 26, 968-981. doi:10.1016/j.drudis.2021.01.007. https://pubmed.ncbi.nlm.nih.gov/33460621/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen