C57BL/6JCya-Gucy1a2em1flox/Cya
Common Name:
Gucy1a2-flox
Product ID:
S-CKO-17837
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
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Basic Information
Strain Name
Gucy1a2-flox
Strain ID
CKOCMP-234889-Gucy1a2-B6J-VB
Gene Name
Product ID
S-CKO-17837
Gene Alias
6330407I18Rik; A230060L24Rik
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
9
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Gucy1a2em1flox/Cya mice (Catalog S-CKO-17837) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000115733
NCBI RefSeq
NM_001033322
Target Region
Exon 2~3
Size of Effective Region
~4.4 kb
Detailed Document
Overview of Gene Research
Gucy1a2, encoding the soluble guanylyl cyclase (sGC) α2 subunit, is involved in the nitric oxide (NO)-cyclic guanosine phosphate (cGMP) signaling pathway, which plays crucial roles in blood pressure regulation, neurotransmitter delivery, renal function, and tumorigenesis [2].
In cerebral ischemia-reperfusion (I/R) injury, circ-Gucy1a2 was down-regulated in mouse brain tissues. Overexpression of circ-Gucy1a2 increased neuronal proliferation, mitigated apoptosis, mitochondrial membrane potential (MMP) loss, and oxidative stress in oxygen-glucose deprivation/reoxygenation (OGD/R) models, protecting mice from CI/RI [1].
In gastric cancer, GUCY1A2 was highly expressed, and its high expression was associated with histological grade, T stage, and poor prognosis. Gene set enrichment analysis (GSEA) showed it was enriched in tumor-associated signaling pathways [2].
A heterozygous gain-of-function variant in GUCY1A2 may cause autonomous ovarian hyperfunction, as in vitro activity assays revealed increased nitric oxide affinity for the mutant enzyme [3].
In infantile hemangiomas (IHs), GUCY1A2 was identified as one of the five diagnostic genes, and single-gene GSEA indicated it inhibited the "valine, leucine, and isoleucine degradation" pathway in IHs development [4].
In conclusion, Gucy1a2 is involved in multiple biological processes and diseases. The study of Gucy1a2, especially through model-based research such as in the CI/RI mouse model, helps to understand its functions in disease mechanisms, providing potential targets for treating cerebral ischemia-reperfusion injury, gastric cancer, ovarian hyperfunction, and IHs.
References：
1. Wang, Fei, Ma, Qian, Dong, Xinglu, Wang, Tinghuan, Ma, Chao. . circ-Gucy1a2 Protects Mice from Cerebral Ischemia-Reperfusion Injury by Attenuating Neuronal Apoptosis and Mitochondrial Membrane Potential Loss. In Journal of investigative surgery : the official journal of the Academy of Surgical Research, 36, 2152509. doi:10.1080/08941939.2022.2152509. https://pubmed.ncbi.nlm.nih.gov/37277119/
2. Li, Xin, Chen, Xiaowei, Hu, Xueju, Wu, Leilei, Shen, Xiaobing. 2021. Overexpression of GUCY1A2 Correlates With Poor Prognosis in Gastric Cancer Patients. In Frontiers in oncology, 11, 632172. doi:10.3389/fonc.2021.632172. https://pubmed.ncbi.nlm.nih.gov/34113559/
3. Wittrien, Theresa, Ziegler, Alban, Rühle, Anne, Coutant, Régis, Behrends, Sönke. . Heterozygous gain of function variant in GUCY1A2 may cause autonomous ovarian hyperfunction. In European journal of endocrinology, 190, 266-274. doi:10.1093/ejendo/lvae030. https://pubmed.ncbi.nlm.nih.gov/38578777/
4. Huang, Sicong, Chen, Ruiqi, Gao, Shihua, Shen, Gang, Jia, Hongyun. 2022. Identification of Diagnostic Markers in Infantile Hemangiomas. In Journal of oncology, 2022, 9395876. doi:10.1155/2022/9395876. https://pubmed.ncbi.nlm.nih.gov/36504560/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen