C57BL/6JCya-Birc2em1flox/Cya
Common Name:
Birc2-flox
Product ID:
S-CKO-17938
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Birc2-flox
Strain ID
CKOCMP-11797-Birc2-B6J-VB
Gene Name
Product ID
S-CKO-17938
Gene Alias
Api1; Api2; Birc3; C-IAP1; C330006D17Rik; HIAP1; HIAP2; IAP1; IAP2; MIAP1; MIAP2; MIHB; MIHC; RNF48; cIAP1; cIAP2; mcIAP1
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
9
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Birc2em1flox/Cya mice (Catalog S-CKO-17938) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000190341
NCBI RefSeq
NM_007465
Target Region
Exon 5~8
Size of Effective Region
~4.2 kb
Detailed Document
Overview of Gene Research
Birc2, also known as baculoviral IAP repeat-containing 2, is a gene involved in regulating fundamental cell death and survival signaling pathways [5]. It can interact with other proteins, and its functions may relate to processes like immune response modulation and cell cycle regulation. The gene's role has been studied through various models to understand its biological importance in different physiological and pathological conditions.
Knockdown of BIRC2 in mouse melanoma or breast cancer cells increases the expression of chemokine CXCL9, impairs tumor growth by augmenting intratumoral activated CD8+ T cells and natural killer cells, and enhances the sensitivity of these tumors to anti-CTLA4 and/or anti-PD1 immune checkpoint blockade (ICB), indicating its role in cancer immune evasion and resistance to ICB [1]. In LPS-mediated C28/I2 cells (an in vitro rheumatoid arthritis (RA) cellular model), BIRC2 knockdown alleviates necroptosis, oxidative stress, and inflammation, and BIRC2 positively regulates TRADD expression, suggesting its potential as a novel target for RA treatment [2]. In hepatocellular carcinoma (HCC), BIRC2 promotes the ubiquitination-dependent degradation of NFκB-inducing kinase (NIK), inactivating the non-canonical NFκB signaling pathway, decreasing MHC-I expression, and protecting HCC cells from T cell-mediated cytotoxicity. Silencing or inhibiting BIRC2 increases the sensitivity of HCC cells to immune killing and improves the function of T cells, enhancing the efficacy of anti-PD-1 therapy [3]. In α7-HPV-related cervical squamous cell carcinoma (SCC), miR-143-3p targets BIRC2, and high BIRC2 expression is associated with poor outcomes. A combination of BIRC2-inhibitor LCL161 and topotecan shows synergistic effects on cancer cells and animal tumor models [4].
In conclusion, Birc2 plays crucial roles in multiple disease-related biological processes. Gene-knockout or knockdown models have revealed its functions in cancer immune evasion, rheumatoid arthritis progression, and HCC immune escape. Understanding Birc2's functions through these models provides potential therapeutic targets for cancer, rheumatoid arthritis, and other related diseases.
References:
1. Samanta, Debangshu, Huang, Tina Yi-Ting, Shah, Rima, Pan, Fan, Semenza, Gregg L. . BIRC2 Expression Impairs Anti-Cancer Immunity and Immunotherapy Efficacy. In Cell reports, 32, 108073. doi:10.1016/j.celrep.2020.108073. https://pubmed.ncbi.nlm.nih.gov/32846130/
2. Rao, Yanting, Xu, Shengjing, Lu, Ting, Liu, Manman, Zhang, Wei. . Downregulation of BIRC2 hinders the progression of rheumatoid arthritis through regulating TRADD. In Immunity, inflammation and disease, 11, e978. doi:10.1002/iid3.978. https://pubmed.ncbi.nlm.nih.gov/37904685/
3. Fu, Lingyi, Li, Shuo, Mei, Jie, Huang, Yuhua, Yun, Jingping. 2025. BIRC2 blockade facilitates immunotherapy of hepatocellular carcinoma. In Molecular cancer, 24, 113. doi:10.1186/s12943-025-02319-5. https://pubmed.ncbi.nlm.nih.gov/40223121/
4. Lin, Chiao-Yun, Wang, Chun-Chieh, Wu, Ren-Chin, Chao, Angel, Lai, Chyong-Huey. 2021. Inhibition of BIRC2 Sensitizes α7-HPV-Related Cervical Squamous Cell Carcinoma to Chemotherapy. In International journal of molecular sciences, 22, . doi:10.3390/ijms222011020. https://pubmed.ncbi.nlm.nih.gov/34681681/
5. Tencer, Adam H, Yu, Yucong, Causse, Sebastien Z, Shi, Xiaobing, Kutateladze, Tatiana G. 2023. Molecular basis for nuclear accumulation and targeting of the inhibitor of apoptosis BIRC2. In Nature structural & molecular biology, 30, 1265-1274. doi:10.1038/s41594-023-01044-1. https://pubmed.ncbi.nlm.nih.gov/37524969/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen