C57BL/6JCya-Chkaem1flox/Cya
Common Name
Chka-flox
Product ID
S-CKO-18025
Backgroud
C57BL/6JCya
Strain ID
CKOCMP-12660-Chka-B6J-VB
When using this mouse strain in a publication, please cite “Chka-flox Mouse (Catalog S-CKO-18025) were purchased from Cyagen.”
Product Type
Age
Genotype
Sex
Quantity
Basic Information
Strain Name
Chka-flox
Strain ID
CKOCMP-12660-Chka-B6J-VB
Gene Name
Product ID
S-CKO-18025
Gene Alias
CK, CK/EK-alpha, Chetk-alpha, Chk, ChoK, EK, EtnK-alpha
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
Chr 19
Phenotype
Datasheet
Application
--
Strain Description
Ensembl Number
ENSMUST00000072055
NCBI RefSeq
NM_013490
Target Region
Exon 3~5
Size of Effective Region
~2.9 kb
Overview of Gene Research
Chka, or choline kinase α, is an enzyme that converts choline to phosphocholine. It is involved in the Kennedy pathways, which catalyze the de novo synthesis of phosphatidylcholine and phosphatidylethanolamine, key components of eukaryotic cell membranes [1].
Bi-allelic variants in CHKA can cause a neurodevelopmental disorder with epilepsy and microcephaly. These variants reduce the enzymatic activity of CHKA, impairing the first step of the Kennedy pathway [1]. In cancer research, CHKA is highly expressed in lung adenocarcinoma, colorectal carcinoma, and glioma, and its elevated expression is associated with poor prognosis. Knockdown of CHKA inhibits the proliferation, invasion, and migration of cancer cells [2,3,5]. In addition, CHKA SNP hCV1562388 genotypes with at least one C allele are associated with a reduced risk of spina bifida [4].
In conclusion, Chka is crucial for the Kennedy pathways in cell membrane synthesis. Studies, especially those on gene variants and knockdown in relevant disease models, have revealed its significance in neurodevelopmental disorders and various cancers, highlighting its potential as a biomarker and therapeutic target.
References：
1. Klöckner, Chiara, Fernández-Murray, J Pedro, Tavasoli, Mahtab, McMaster, Christopher R, Platzer, Konrad. . Bi-allelic variants in CHKA cause a neurodevelopmental disorder with epilepsy and microcephaly. In Brain : a journal of neurology, 145, 1916-1923. doi:10.1093/brain/awac074. https://pubmed.ncbi.nlm.nih.gov/35202461/
2. Zhang, Li, Chen, Ping, Yang, Shen, Wu, Peng, Jiang, Shujuan. 2016. CHKA mediates the poor prognosis of lung adenocarcinoma and acts as a prognostic indicator. In Oncology letters, 12, 1849-1853. doi:. https://pubmed.ncbi.nlm.nih.gov/27588131/
3. Yue, Fangqian, Zou, Yourui, Sun, Shengyu, Huang, Ling, Ma, Hui. . [Knockdown of choline kinase α (CHKA) inhibits the proliferation, invasion and migration of human U87MG glioma cells]. In Xi bao yu fen zi mian yi xue za zhi = Chinese journal of cellular and molecular immunology, 36, 724-728. doi:. https://pubmed.ncbi.nlm.nih.gov/32958130/
4. Enaw, James O Ebot, Zhu, Huiping, Yang, Wei, Lammer, Edward J, Finnell, Richard H. 2006. CHKA and PCYT1A gene polymorphisms, choline intake and spina bifida risk in a California population. In BMC medicine, 4, 36. doi:. https://pubmed.ncbi.nlm.nih.gov/17184542/
5. Hu, Liang, Wang, Ruo-Yu, Cai, Jian, Zhou, Wei-Ping, Cai, Qing-Ping. . Overexpression of CHKA contributes to tumor progression and metastasis and predicts poor prognosis in colorectal carcinoma. In Oncotarget, 7, 66660-66678. doi:10.18632/oncotarget.11433. https://pubmed.ncbi.nlm.nih.gov/27556502/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen
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