C57BL/6JCya-Tigarem1flox/Cya
Common Name:
Tigar-flox
Product ID:
S-CKO-18076
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
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Basic Information
Strain Name
Tigar-flox
Strain ID
CKOCMP-319801-Tigar-B6J-VB
Gene Name
Product ID
S-CKO-18076
Gene Alias
9630033F20Rik
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
6
Phenotype
Document
Application
--
Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Tigarem1flox/Cya mice (Catalog S-CKO-18076) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000039913
NCBI RefSeq
NM_177003
Target Region
Exon 3
Size of Effective Region
~0.9 kb
Detailed Document
Overview of Gene Research
TIGAR, short for TP53-induced glycolysis and apoptosis regulator, is a downstream target gene of p53. It contains a sequence similar to the 6-phosphofructose kinase/fructose-2, 6-bisphosphatase bisphosphatase domain. TIGAR mainly functions as a fructose-2,6-bisphosphatase to inhibit glycolysis, facilitating the pentose phosphate pathway to produce NADPH and ribose. This helps maintain energy metabolism balance, regulate autophagy and stem cell differentiation, and promote cell survival. It is also involved in various biological processes such as anti-oxidative stress, reducing inflammation, and inhibiting apoptosis [3,4].
In murine sepsis models, myeloid Tigar ablation attenuated sepsis-induced inflammation as TIGAR directly binds to TAK1 and promotes its ubiquitination and auto-phosphorylation, highlighting its role in macrophage-orchestrated inflammation [1]. In obese TIGAR transgenic mice, TIGAR exacerbates obesity by triggering LRRK2-mediated defects in macroautophagy and chaperone-mediated autophagy in adipocytes, suggesting its role in lipid metabolism [2]. In gestational diabetes mellitus models, loss of TIGAR in trophoblast cells increased ROS, driving a phenotypic switch and pyroptosis through the NLRP3-ASC-caspase-1 signaling pathway [5].
In conclusion, TIGAR plays essential roles in multiple biological processes including energy metabolism, inflammation, autophagy, and lipid metabolism. Studies using gene knockout or conditional knockout mouse models have revealed its significance in diseases such as sepsis, obesity, and gestational diabetes mellitus, providing potential therapeutic targets for these conditions.
References:
1. Wang, Dongdong, Li, Yanxia, Yang, Hao, Chen, Qi, Ben, Jingjing. 2024. Disruption of TIGAR-TAK1 alleviates immunopathology in a murine model of sepsis. In Nature communications, 15, 4340. doi:10.1038/s41467-024-48708-0. https://pubmed.ncbi.nlm.nih.gov/38773142/
2. Zhang, Tian, Linghu, Ke-Gang, Tan, Jia, Qin, Zheng-Hong, Guo, Bing. 2024. TIGAR exacerbates obesity by triggering LRRK2-mediated defects in macroautophagy and chaperone-mediated autophagy in adipocytes. In Autophagy, 20, 1741-1761. doi:10.1080/15548627.2024.2338576. https://pubmed.ncbi.nlm.nih.gov/38686804/
3. Tang, Jie, Chen, Lei, Qin, Zheng-Hong, Sheng, Rui. 2021. Structure, regulation, and biological functions of TIGAR and its role in diseases. In Acta pharmacologica Sinica, 42, 1547-1555. doi:10.1038/s41401-020-00588-y. https://pubmed.ncbi.nlm.nih.gov/33510458/
4. Huang, Bei, Lang, Xiaoling, Li, Xihong. 2022. The role of TIGAR in nervous system diseases. In Frontiers in aging neuroscience, 14, 1023161. doi:10.3389/fnagi.2022.1023161. https://pubmed.ncbi.nlm.nih.gov/36437984/
5. Guo, Junjun, Zhou, Meijuan, Zhao, Man, Li, Anna, Zhang, Meihua. 2023. TIGAR deficiency induces caspase-1-dependent trophoblasts pyroptosis through NLRP3-ASC inflammasome. In Frontiers in immunology, 14, 1114620. doi:10.3389/fimmu.2023.1114620. https://pubmed.ncbi.nlm.nih.gov/37122710/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen