C57BL/6JCya-Ungem1flox/Cya
Common Name
Ung-flox
Product ID
S-CKO-18079
Backgroud
C57BL/6JCya
Strain ID
CKOCMP-22256-Ung-B6J-VB
When using this mouse strain in a publication, please cite “Ung-flox Mouse (Catalog S-CKO-18079) were purchased from Cyagen.”
Product Type
Age
Genotype
Sex
Quantity
Basic Information
Strain Name
Ung-flox
Strain ID
CKOCMP-22256-Ung-B6J-VB
Gene Name
Product ID
S-CKO-18079
Gene Alias
UNG1, UNG2
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
Chr 5
Phenotype
Datasheet
Application
--
Strain Description
Ensembl Number
ENSMUST00000031587
NCBI RefSeq
NM_001040691
Target Region
Exon 4~5
Size of Effective Region
~1.1 kb
Overview of Gene Research
Ung, short for uracil DNA glycosylase, is a key member of the base excision repair complex. It plays a crucial role in maintaining genomic stability by removing uracils from DNA, initiating high-fidelity base excision repair [2,4]. During B-cell development, it is involved in immunoglobulin (Ig) somatic hypermutation (SHM) and class switch recombination (CSR), processes that are essential for the adaptive immune response [1,2,3,6,7].
In mouse models, AID deficiency abolishes both CSR and SHM, while UNG-deficient mice have drastically reduced CSR but augmented SHM, suggesting differential functions of UNG in these processes [1]. The UNG-RPA interaction has been found to govern the choice between high-fidelity and mutagenic uracil repair. Mutants of UNG or RPA unable to interact only support high-fidelity repair, with transversions at the Ig variable region abated while CSR is still supported [2]. Also, UNG deficiency reduces B-cell clonal expansion in the germinal center in mice and blocks the proliferation of tumor B cells expressing AID, indicating its role in protecting B cells from AID-induced telomere loss [5].
In conclusion, Ung is essential for DNA repair, especially in maintaining genomic stability during B-cell development and antibody diversification. The study of Ung-deficient mouse models has provided valuable insights into its role in CSR, SHM, and protection against B-cell lymphoma, highlighting its significance in understanding the mechanisms of immune-related diseases and potentially guiding the development of novel therapeutic strategies [1,2,5].
References：
1. Yousif, Ashraf S, Stanlie, Andre, Begum, Nasim A, Honjo, Tasuku. 2014. Opinion: uracil DNA glycosylase (UNG) plays distinct and non-canonical roles in somatic hypermutation and class switch recombination. In International immunology, 26, 575-8. doi:10.1093/intimm/dxu071. https://pubmed.ncbi.nlm.nih.gov/24994819/
2. Mu, Yunxiang, Chen, Zaowen, Plummer, Joshua B, Krug, Laurie T, McBride, Kevin M. 2024. UNG-RPA interaction governs the choice between high-fidelity and mutagenic uracil repair. In bioRxiv : the preprint server for biology, , . doi:10.1101/2024.04.30.591927. https://pubmed.ncbi.nlm.nih.gov/38746347/
3. Hayran, Abdul B, Liabakk, Nina B, Aas, Per A, Slupphaug, Geir, Kavli, Bodil. . RPA guides UNG to uracil in ssDNA to facilitate antibody class switching and repair of mutagenic uracil at the replication fork. In Nucleic acids research, 52, 784-800. doi:10.1093/nar/gkad1115. https://pubmed.ncbi.nlm.nih.gov/38000394/
4. Krokan, H E, Otterlei, M, Nilsen, H, Aas, P A, Slupphaug, G. . Properties and functions of human uracil-DNA glycosylase from the UNG gene. In Progress in nucleic acid research and molecular biology, 68, 365-86. doi:. https://pubmed.ncbi.nlm.nih.gov/11554311/
5. Cortizas, Elena M, Zahn, Astrid, Safavi, Shiva, Di Noia, Javier M, Verdun, Ramiro E. 2016. UNG protects B cells from AID-induced telomere loss. In The Journal of experimental medicine, 213, 2459-2472. doi:. https://pubmed.ncbi.nlm.nih.gov/27697833/
6. Martin, Ophélie A, Thomas, Morgane, Marquet, Marie, Le Noir, Sandrine, Pinaud, Eric. 2023. The IgH Eµ-MAR regions promote UNG-dependent error-prone repair to optimize somatic hypermutation. In Frontiers in immunology, 14, 1030813. doi:10.3389/fimmu.2023.1030813. https://pubmed.ncbi.nlm.nih.gov/36865553/
7. Pérez-Durán, Pablo, Belver, Laura, de Yébenes, Virginia G, Pisano, David G, Ramiro, Almudena R. 2012. UNG shapes the specificity of AID-induced somatic hypermutation. In The Journal of experimental medicine, 209, 1379-89. doi:10.1084/jem.20112253. https://pubmed.ncbi.nlm.nih.gov/22665573/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen
Contact Us
Connect with our experts for your custom animal model needs. Please fill out the form below to start a conversation or request a quote.
Cyagen values your privacy. We’d like to keep you informed about our latest offerings and insights. Your preferences:
You may unsubscribe from these communications at any time. See our Privacy Policy for details on opting out and data protection.
By clicking the button below, you consent to allow Cyagen to store and process the personal information submitted in this form to provide you the content requested.