C57BL/6JCya-Apoa4em1flox/Cya
Common Name:
Apoa4-flox
Product ID:
S-CKO-18200
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Apoa4-flox
Strain ID
CKOCMP-11808-Apoa4-B6J-VB
Gene Name
Product ID
S-CKO-18200
Gene Alias
Apoa-4; apo-AIV; apoA-IV
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
9
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Apoa4em1flox/Cya mice (Catalog S-CKO-18200) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000034585
NCBI RefSeq
NM_007468
Target Region
Exon 3
Size of Effective Region
~2.1 kb
Detailed Document
Overview of Gene Research
Apoa4, short for apolipoprotein A-IV, is one of the most abundant and versatile apolipoproteins facilitating lipid transport and metabolism [5]. It is synthesized in the small intestine, packaged onto chylomicrons, and then transported to several tissues. Apoa4 has also been identified as a sphingosine 1-phosphate chaperone, supporting extracellular signaling functions [6].
Mutations in Apoa4 can cause autosomal dominant medullary amyloidosis, presenting as tubulointerstitial kidney disease with a bland urinary sediment [1]. Serum Apoa4 levels are increased after administration of recombinant human hepatocyte growth factor (rh-HGF), making it a potential pharmacodynamic marker of rh-HGF [2]. In Stevens-Johnson syndrome/toxic epidermal necrolysis, Apoa4 may serve as a novel prognostic marker [3]. Also, in T2DM patients, serum Apoa4 levels are associated with the progression of renal impairment [4].
In conclusion, Apoa4 plays essential roles in lipid metabolism, extracellular signaling, and potentially in drug-response prediction. Studies on Apoa4-related diseases such as kidney-related amyloidosis, renal impairment in T2DM, and some skin diseases provide valuable insights into understanding these disease mechanisms.
References：
1. Kmochová, Tereza, Kidd, Kendrah O, Orr, Andrew, Bleyer, Anthony J, Kmoch, Stanislav. 2023. Autosomal dominant ApoA4 mutations present as tubulointerstitial kidney disease with medullary amyloidosis. In Kidney international, 105, 799-811. doi:10.1016/j.kint.2023.11.021. https://pubmed.ncbi.nlm.nih.gov/38096951/
2. Motoi, Sotaro, Uesugi, Mai, Obara, Takashi, Imai, Toshio, Kawano, Tetsu. 2021. Serum APOA4 Pharmacodynamically Represents Administered Recombinant Human Hepatocyte Growth Factor (E3112). In International journal of molecular sciences, 22, . doi:10.3390/ijms22094578. https://pubmed.ncbi.nlm.nih.gov/33925510/
3. Gong, Ting, Zhang, Peng, Ruan, Shi-Fan, Chung, Wen-Hung, Ji, Chao. 2023. APOA4 as a novel predictor of prognosis in Stevens-Johnson syndrome/toxic epidermal necrolysis: A proteomics analysis from two prospective cohorts. In Journal of the American Academy of Dermatology, 89, 45-52. doi:10.1016/j.jaad.2023.02.058. https://pubmed.ncbi.nlm.nih.gov/36963506/
4. Cheng, Chao-Wen, Chang, Che-Chang, Chen, Hsiu-Wen, Lin, Ching-Yu, Chen, Jin-Shuen. 2018. Serum ApoA4 levels predicted the progression of renal impairment in T2DM. In European journal of clinical investigation, 48, e12937. doi:10.1111/eci.12937. https://pubmed.ncbi.nlm.nih.gov/29675916/
5. Qu, Jie, Fourman, Sarah, Fitzgerald, Maureen, Tso, Patrick, Bhargava, Aditi. 2021. Low-density lipoprotein receptor-related protein 1 (LRP1) is a novel receptor for apolipoprotein A4 (APOA4) in adipose tissue. In Scientific reports, 11, 13289. doi:10.1038/s41598-021-92711-0. https://pubmed.ncbi.nlm.nih.gov/34168225/
6. Obinata, Hideru, Kuo, Andrew, Wada, Yukata, Izumi, Takashi, Hla, Timothy. 2019. Identification of ApoA4 as a sphingosine 1-phosphate chaperone in ApoM- and albumin-deficient mice. In Journal of lipid research, 60, 1912-1921. doi:10.1194/jlr.RA119000277. https://pubmed.ncbi.nlm.nih.gov/31462513/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen