C57BL/6JCya-Latem1flox/Cya
Common Name:
Lat-flox
Product ID:
S-CKO-18207
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
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Basic Information
Strain Name
Lat-flox
Strain ID
CKOCMP-16797-Lat-B6J-VB
Gene Name
Product ID
S-CKO-18207
Gene Alias
p36-38; pp36
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
7
Phenotype
Document
Application
--
Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Latem1flox/Cya mice (Catalog S-CKO-18207) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000032997
NCBI RefSeq
NM_010689
Target Region
Exon 9~12
Size of Effective Region
~2.6 kb
Detailed Document
Overview of Gene Research
LAT, also known as linker for activation of T cells, is a crucial transmembrane adapter protein. It plays a central role in T-cell antigenic signaling, acting as a key bridge between T cell-specific and general signaling pathways [2,3,4]. TCR activation is relayed via LAT, which organizes protein partners and mediates signal propagation down diverse pathways such as NFAT and AP-1 [1]. LAT is also involved in the function and differentiation of T cells, and its mutations can lead to abnormal T-cell development and function [2,5,6].
Mouse models with mutant forms of LAT have provided valuable insights. Some mutant mice showed partial or complete inhibition of T-cell development, indicating LAT's role as a positive regulator of TCR signaling [5,6]. However, LAT "knock-ins" with point mutations in the four COOH-terminal tyrosine residues developed lymphoproliferative disorders involving polyclonal T cells that produced high amounts of Th2 cytokines, revealing LAT as a negative regulator of TCR signaling and T-cell homeostasis [5,6].
In conclusion, LAT is essential for T-cell activation pathway balance and T-cell homeostasis. Mouse models, especially those with LAT mutations, have significantly contributed to understanding its role in T-cell-related immunopathologies, highlighting its importance in immunological research and potentially in developing treatments for related diseases.
References:
1. Rubin, Adam J, Dao, Tyler T, Schueppert, Amelia V, Regev, Aviv, Shalek, Alex K. 2024. LAT encodes T cell activation pathway balance. In bioRxiv : the preprint server for biology, , . doi:10.1101/2024.08.26.609683. https://pubmed.ncbi.nlm.nih.gov/39253472/
2. Balagopalan, Lakshmi, Coussens, Nathan P, Sherman, Eilon, Samelson, Lawrence E, Sommers, Connie L. 2010. The LAT story: a tale of cooperativity, coordination, and choreography. In Cold Spring Harbor perspectives in biology, 2, a005512. doi:10.1101/cshperspect.a005512. https://pubmed.ncbi.nlm.nih.gov/20610546/
3. Wange, R L. 2000. LAT, the linker for activation of T cells: a bridge between T cell-specific and general signaling pathways. In Science's STKE : signal transduction knowledge environment, 2000, re1. doi:. https://pubmed.ncbi.nlm.nih.gov/11752630/
4. Wonerow, P, Watson, S P. . The transmembrane adapter LAT plays a central role in immune receptor signalling. In Oncogene, 20, 6273-83. doi:. https://pubmed.ncbi.nlm.nih.gov/11607829/
5. Malissen, Bernard, Aguado, Enrique, Malissen, Marie. . Role of the LAT adaptor in T-cell development and Th2 differentiation. In Advances in immunology, 87, 1-25. doi:. https://pubmed.ncbi.nlm.nih.gov/16102570/
6. Malissen, Bernard, Wang, Ying, Mingueneau, Michael, Malissen, Marie. . Th2 lymphoproliferative disorders resulting from defective LAT signalosomes. In Novartis Foundation symposium, 281, 93-100; discussion 100-2, 208-9. doi:. https://pubmed.ncbi.nlm.nih.gov/17534068/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen