C57BL/6JCya-Trpm5em1flox/Cya
Common Name:
Trpm5-flox
Product ID:
S-CKO-18210
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Trpm5-flox
Strain ID
CKOCMP-56843-Trpm5-B6J-VB
Gene Name
Product ID
S-CKO-18210
Gene Alias
9430099A16Rik; LTrpC-5; Ltrpc5; Mtr1
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
7
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Trpm5em1flox/Cya mice (Catalog S-CKO-18210) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000009390
NCBI RefSeq
NM_020277.2
Target Region
Exon 4
Size of Effective Region
~673 bp
Detailed Document
Overview of Gene Research
Trpm5 is a Ca(2+)-activated cation channel, essential for transducing bitter, sweet, and umami tastes [1,3]. It is the final component in a signaling cascade initiated by the activation of G protein-coupled receptors, requiring the enzyme PLCβ2 [1]. The distribution of Trpm5 spans from chemosensory cells in the digestive, respiratory, and olfactory systems to pancreatic islets, where it contributes to insulin secretion [1]. Gene knockout mouse models have been crucial in its functional studies [2].
Trpm5 -/- mice lack type II taste perception and exhibit reduced glucose-induced insulin secretion [2]. In lipotoxicity-induced pancreatic β-cell dysfunction, high-fat-diet feeding and palmitate treatment decrease TRPM5 expression in mouse pancreatic islets and MIN6 cells. Knocking down Trpm5 inhibits insulin secretion, while overexpressing it reverses lipotoxicity-induced glucose-stimulated insulin secretion (GSIS) defects [4]. In B cells, Trpm5-deficient mice show enhanced proliferation and increased production of inflammatory cytokines in response to lipopolysaccharide (LPS) stimulation due to increased cytosolic calcium concentration [5].
In conclusion, Trpm5 plays vital roles in taste transduction, insulin secretion, and regulation of calcium-dependent responses in B cells. Studies using Trpm5 knockout mouse models have revealed its significance in diseases such as type II diabetes and metabolic syndrome, as well as in endotoxic shock [2,5].
References:
1. Liman, Emily R. . TRPM5. In Handbook of experimental pharmacology, 222, 489-502. doi:10.1007/978-3-642-54215-2_19. https://pubmed.ncbi.nlm.nih.gov/24756718/
2. Vennekens, R, Mesuere, M, Philippaert, K. 2017. TRPM5 in the battle against diabetes and obesity. In Acta physiologica (Oxford, England), 222, . doi:10.1111/apha.12949. https://pubmed.ncbi.nlm.nih.gov/28834354/
3. Liman, E R. . TRPM5 and taste transduction. In Handbook of experimental pharmacology, , 287-98. doi:. https://pubmed.ncbi.nlm.nih.gov/17217064/
4. Wang, Kai-Yuan, Wu, Shi-Mei, Yao, Zheng-Jian, Zhu, Yun-Xia, Han, Xiao. 2024. Insufficient TRPM5 Mediates Lipotoxicity-induced Pancreatic β-cell Dysfunction. In Current medical science, 44, 346-354. doi:10.1007/s11596-023-2795-5. https://pubmed.ncbi.nlm.nih.gov/38517672/
5. Sakaguchi, Taiki, Okumura, Ryu, Ono, Chisato, Baba, Yoshihiro, Takeda, Kiyoshi. . TRPM5 Negatively Regulates Calcium-Dependent Responses in Lipopolysaccharide-Stimulated B Lymphocytes. In Cell reports, 31, 107755. doi:10.1016/j.celrep.2020.107755. https://pubmed.ncbi.nlm.nih.gov/32521253/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen