C57BL/6JCya-Crotem1flox/Cya
Common Name:
Crot-flox
Product ID:
S-CKO-18296
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Crot-flox
Strain ID
CKOCMP-74114-Crot-B6J-VB
Gene Name
Product ID
S-CKO-18296
Gene Alias
1200003H03Rik
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
5
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Crotem1flox/Cya mice (Catalog S-CKO-18296) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000003720
NCBI RefSeq
NM_023733
Target Region
Exon 4
Size of Effective Region
~0.6 kb
Detailed Document
Overview of Gene Research
Crot, short for Carnitine O-octanoyltransferase, is a peroxisomal enzyme involved in liver fatty acid oxidation [1,2]. It has also been associated with pathways related to vascular calcification, lipid metabolism, and potentially the TGF-β signaling pathway in ovarian cancer [2,3]. Genetic models, such as knockout mice, have been crucial in understanding its functions.
In Crot-deficient (Crot-/-) mice, metabolomic analysis showed increased levels of omega-3 fatty acids like EPA, DPA, and DHA in the liver, and EPA in the plasma. Also, anti-inflammatory dicarboxylic acids were higher in the plasma of Crot-/-mice, suggesting a potential anti-calcification mechanism of Crot suppression [1]. In addition, Nuclease technology-mediated Crot deficiency in LDL receptor-deficient mice reduced aortic and carotid artery calcification without affecting bone density or lipid levels in the liver and plasma, indicating Crot promotes vascular calcification via fatty acid metabolism and mitochondrial dysfunction [2].
In conclusion, Crot plays a role in fatty acid metabolism and is involved in processes related to vascular calcification. The study of Crot-deficient mouse models has provided insights into its function in these disease-relevant processes, suggesting Crot inhibition could be a potential antifibrocalcific therapy [2].
References:
1. Okui, Takehito, Kuraoka, Shiori, Iwashita, Masaya, Singh, Sasha A, Aikawa, Elena. 2024. Carnitine O-octanoyltransferase (CROT) deficiency in mice leads to an increase of omega-3 fatty acids. In Frontiers in molecular biosciences, 11, 1374316. doi:10.3389/fmolb.2024.1374316. https://pubmed.ncbi.nlm.nih.gov/39076376/
2. Okui, Takehito, Iwashita, Masaya, Rogers, Maximillian A, Singh, Sasha A, Aikawa, Elena. 2020. CROT (Carnitine O-Octanoyltransferase) Is a Novel Contributing Factor in Vascular Calcification via Promoting Fatty Acid Metabolism and Mitochondrial Dysfunction. In Arteriosclerosis, thrombosis, and vascular biology, 41, 755-768. doi:10.1161/ATVBAHA.120.315007. https://pubmed.ncbi.nlm.nih.gov/33356393/
3. Li, Xin, Gao, Xuzhu, Yuan, Jia, Zhang, Jihong, Xu, Guoxiong. 2022. The miR-33a-5p/CROT axis mediates ovarian cancer cell behaviors and chemoresistance via the regulation of the TGF-β signal pathway. In Frontiers in endocrinology, 13, 950345. doi:10.3389/fendo.2022.950345. https://pubmed.ncbi.nlm.nih.gov/36120434/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen