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C57BL/6JCya-Ap2b1em1flox/Cya
Common Name:
Ap2b1-flox
Product ID:
S-CKO-18325
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Ap2b1-flox
Strain ID
CKOCMP-71770-Ap2b1-B6J-VB
Gene Name
Ap2b1
Product ID
S-CKO-18325
Gene Alias
1300012O03Rik; AP105B
Background
C57BL/6JCya
NCBI ID
71770
Modification
Conditional knockout
Chromosome
11
Phenotype
MGI:1919020
Document
Click here to download >>
Application
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Rare Disease Data Center >>
Note
Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Ap2b1em1flox/Cya mice (Catalog S-CKO-18325) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000018875
NCBI RefSeq
NM_001035854
Target Region
Exon 4
Size of Effective Region
~1.1 kb
Detailed Document
Click here to download >>
Overview of Gene Research
Ap2b1, also known as adaptor-related protein complex 2 subunit beta 1, is involved in endocytosis, a process crucial for cells to take in substances from their environment. It is part of the AP-2 complex, which plays a role in clathrin-mediated endocytosis, a major pathway for internalizing extracellular materials. This gene is important in maintaining normal cellular function and has implications in various biological processes [5].

In the context of high-altitude cerebral edema (HACE), NRF1 up-regulates Ap2B1 in activated microglia under hypoxia. This enhances the phagocytic function of microglia, which is part of the mechanism leading to HACE [1]. In a male mouse model of noise-induced sensorineural hearing loss (SNHL), miR-145b negatively regulates Ap2b1, and depletion of miR-145b can alleviate auditory threshold shifts and outer hair cell loss, suggesting Ap2b1's role in SNHL [2]. For influenza A virus entry, SLC35B4 affects the expression level of Ap2B1 through HS modification-mediated AGRN protein homeostasis, influencing virus internalization [3]. The G protein-coupled receptor FFAR2 promotes influenza A virus entry via the FFAR2-β-arrestin1-AP2B1 signaling cascade, with knockdown of AP2B1 impairing virus replication [5]. In the Zika virus infection, LINC08148 knockout down-regulates the transcription level of Ap2B1, and among other genes, loss of Src (which is up-regulated by LINC08148) significantly decreases the uptake of Zika virus, indicating Ap2B1's role in the virus entry process [4].

In Parkinson's disease and related disorders, lower levels of AP2B1 were observed in multiple system atrophy (MSA) and progressive supranuclear palsy (PSP) compared to healthy controls, suggesting its potential as a biomarker for synaptic dysfunction [6]. In triple-negative breast cancer, overexpression of AP2B1 (a downstream target of ZHX2 and HIF1α) could partially rescue the cell growth defect caused by ZHX2 depletion, highlighting its role in cancer cell growth [7]. In major depressive disorder, AP2B1 was identified as a hub gene in the protein-protein interaction network of differentially expressed genes, potentially serving as a therapeutic target [8]. In the study of human umbilical cord mesenchymal stem cell (hUC-MSC) extracellular vesicles for treating Alzheimer's disease, AP2B1 in hUC-MSC-derived EVs may play a role in regulating the synaptic vesicle cycle signalling pathway [9].

In summary, Ap2b1 is mainly involved in endocytosis-related processes and has been shown to play important roles in various disease conditions such as HACE, SNHL, viral infections, neurodegenerative diseases, cancer, and mental disorders. The use of mouse models in these studies has helped to uncover the functions of Ap2b1 in different biological processes and disease mechanisms, providing potential targets for treatment and new insights into disease pathogenesis.

References:
1. Wang, Xueting, Chen, Guijuan, Wan, Baolan, Lu, Yapeng, Zhu, Li. . NRF1-mediated microglial activation triggers high-altitude cerebral edema. In Journal of molecular cell biology, 14, . doi:10.1093/jmcb/mjac036. https://pubmed.ncbi.nlm.nih.gov/35704676/
2. Gu, Xiang, Jiang, Mengxian, Chen, Wei. 2025. miR-145b/AP2B1 Axis Contributes to Noise-induced Sensorineural Hearing Loss In a Male Mouse Model. In Cell biochemistry and biophysics, , . doi:10.1007/s12013-024-01665-3. https://pubmed.ncbi.nlm.nih.gov/39813009/
3. Wang, Guangwen, Jiang, Li, Yan, Ya, Chen, Hualan, Li, Chengjun. 2025. Cellular SLC35B4 promotes internalization during influenza A virus entry. In mBio, 16, e0019425. doi:10.1128/mbio.00194-25. https://pubmed.ncbi.nlm.nih.gov/40130891/
4. Huo, Zhiting, Zhu, Xuanfeng, Peng, Qinyu, Liu, Chao, Zhang, Ping. 2024. LINC08148 promotes the caveola-mediated endocytosis of Zika virus through upregulating transcription of Src. In Journal of virology, 98, e0170523. doi:10.1128/jvi.01705-23. https://pubmed.ncbi.nlm.nih.gov/38742902/
5. Wang, Guangwen, Jiang, Li, Wang, Jinliang, Chen, Hualan, Li, Chengjun. 2020. The G Protein-Coupled Receptor FFAR2 Promotes Internalization during Influenza A Virus Entry. In Journal of virology, 94, . doi:10.1128/JVI.01707-19. https://pubmed.ncbi.nlm.nih.gov/31694949/
6. Nilsson, Johanna, Constantinescu, Julius, Nellgård, Bengt, Bäckström, David, Brinkmalm, Ann. 2022. Cerebrospinal Fluid Biomarkers of Synaptic Dysfunction are Altered in Parkinson's Disease and Related Disorders. In Movement disorders : official journal of the Movement Disorder Society, 38, 267-277. doi:10.1002/mds.29287. https://pubmed.ncbi.nlm.nih.gov/36504237/
7. Fang, Wentong, Liao, Chengheng, Shi, Rachel, Yang, Lee-Wei, Zhang, Qing. 2021. ZHX2 promotes HIF1α oncogenic signaling in triple-negative breast cancer. In eLife, 10, . doi:10.7554/eLife.70412. https://pubmed.ncbi.nlm.nih.gov/34779768/
8. Feng, Jianfei, Zhou, Qing, Gao, Wenquan, Wu, Yanying, Mu, Ruibin. 2019. Seeking for potential pathogenic genes of major depressive disorder in the Gene Expression Omnibus database. In Asia-Pacific psychiatry : official journal of the Pacific Rim College of Psychiatrists, 12, e12379. doi:10.1111/appy.12379. https://pubmed.ncbi.nlm.nih.gov/31889427/
9. Li, Shuang, Zhang, Jiayi, Liu, Xinxing, Guo, Chunyan, Liu, Xifu. 2024. Proteomic characterization of hUC-MSC extracellular vesicles and evaluation of its therapeutic potential to treat Alzheimer's disease. In Scientific reports, 14, 5959. doi:10.1038/s41598-024-56549-6. https://pubmed.ncbi.nlm.nih.gov/38472335/
Quality Control Standard
Sperm Test

Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.

Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.

Environmental Standards:SPF
Available Region:Global
Source:Cyagen
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