C57BL/6JCya-Sephs1em1flox/Cya
Common Name:
Sephs1-flox
Product ID:
S-CKO-18396
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Sephs1-flox
Strain ID
CKOCMP-109079-Sephs1-B6J-VB
Gene Name
Product ID
S-CKO-18396
Gene Alias
1110046B24Rik; SELD; SPS; SPS1
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
2
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Sephs1em1flox/Cya mice (Catalog S-CKO-18396) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000027973
NCBI RefSeq
NM_175400
Target Region
Exon 3
Size of Effective Region
~1.4 kb
Detailed Document
Overview of Gene Research
SEPHS1, or Selenophosphate Synthetase 1, is a gene encoding an enzyme that was initially thought to be involved in selenophosphate synthesis, which is crucial for selenoprotein biosynthesis [1,2,5]. Selenoproteins are vital for antioxidant defense, thyroid hormone metabolism, and cellular homeostasis [1]. Although phylogenetic analysis suggests SEPHS1 lost selenophosphate synthesis activity during evolution, it retains ATPase activity [2]. It is also implicated in the regulation of cellular redox homeostasis [2].
In various KO mouse models, SEPHS1 deficiency has shown significant impacts. In adult mice, cartilage-specific Sephs1 knockout causes aging-associated osteoarthritis and augments post-traumatic OA, due to impaired selenoprotein synthesis, elevated ROS levels, and chondrocyte senescence [3]. In mouse embryos, Sephs1 deficiency affects retinoic signaling and other related pathways, leading to embryonic lethality at E11.5 [2]. In mouse embryonic stem cells, Sephs1 KO shows little effect on pluripotency maintenance but impairs differentiation into three germ layers and gastruloid aggregation, especially cardiac differentiation [4].
In conclusion, SEPHS1 is essential for maintaining cellular redox homeostasis and plays a crucial role in multiple biological processes such as embryonic development, cartilage health, and stem cell differentiation. Studies using KO mouse models have revealed its significance in diseases like osteoarthritis and neurodevelopmental disorders, highlighting its potential as a therapeutic target [1,3,6].
References:
1. Ahmed Mohamed, Zakaria, Yang, Jianli, Wen, Jianping, Jia, Feiyong, Banerjee, Santasree. 2024. SEPHS1 Gene: A new master key for neurodevelopmental disorders. In Clinica chimica acta; international journal of clinical chemistry, 562, 119844. doi:10.1016/j.cca.2024.119844. https://pubmed.ncbi.nlm.nih.gov/38960024/
2. Bang, Jeyoung, Kang, Donghyun, Jung, Jisu, Kim, Jin-Hong, Lee, Byeong Jae. 2022. SEPHS1: Its evolution, function and roles in development and diseases. In Archives of biochemistry and biophysics, 730, 109426. doi:10.1016/j.abb.2022.109426. https://pubmed.ncbi.nlm.nih.gov/36202216/
3. Kang, Donghyun, Lee, Jeeyeon, Jung, Jisu, Lee, Byeong Jae, Kim, Jin-Hong. 2022. Selenophosphate synthetase 1 deficiency exacerbates osteoarthritis by dysregulating redox homeostasis. In Nature communications, 13, 779. doi:10.1038/s41467-022-28385-7. https://pubmed.ncbi.nlm.nih.gov/35140209/
4. Qiao, Lu, Dho, So Hee, Kim, Ji Young, Kim, Lark Kyun. 2021. SEPHS1 is dispensable for pluripotency maintenance but indispensable for cardiac differentiation in mouse embryonic stem cells. In Biochemical and biophysical research communications, 590, 125-131. doi:10.1016/j.bbrc.2021.12.091. https://pubmed.ncbi.nlm.nih.gov/34974300/
5. Na, Jiwoon, Jung, Jisu, Bang, Jeyoung, Hatfield, Dolph L, Lee, Byeong Jae. 2018. Selenophosphate synthetase 1 and its role in redox homeostasis, defense and proliferation. In Free radical biology & medicine, 127, 190-197. doi:10.1016/j.freeradbiomed.2018.04.577. https://pubmed.ncbi.nlm.nih.gov/29715549/
6. Mullegama, Sureni V, Kiernan, Kaitlyn A, Torti, Erin, Yang, Jun, Juusola, Jane. 2024. De novo missense variants in exon 9 of SEPHS1 cause a neurodevelopmental condition with developmental delay, poor growth, hypotonia, and dysmorphic features. In American journal of human genetics, 111, 778-790. doi:10.1016/j.ajhg.2024.02.016. https://pubmed.ncbi.nlm.nih.gov/38531365/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen