C57BL/6JCya-Mestem1flox/Cya
Common Name
Mest-flox
Product ID
S-CKO-18537
Backgroud
C57BL/6JCya
Strain ID
CKOCMP-17294-Mest-B6J-VB
When using this mouse strain in a publication, please cite “Mest-flox Mouse (Catalog S-CKO-18537) were purchased from Cyagen.”
Product Type
Age
Genotype
Sex
Quantity
Basic Information
Strain Name
Mest-flox
Strain ID
CKOCMP-17294-Mest-B6J-VB
Gene Name
Product ID
S-CKO-18537
Gene Alias
Peg1
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
Chr 6
Phenotype
Datasheet
Application
--
Strain Description
Ensembl Number
ENSMUST00000124665
NCBI RefSeq
NM_001252292.1
Target Region
Exon 4~8
Size of Effective Region
~3.0 kb
Overview of Gene Research
Mest, also known as Mesoderm-specific transcript, is a gene with significant implications in multiple biological processes. It has been associated with the regulation of cell proliferation, migration, and invasion in cancer-related pathways. For example, it is involved in the IL-6/JAK/STAT3/Twist-1 signal pathway in breast cancer and the STAT3/Twist-1-mediated EMT in bladder cancer [2,3].
In cancer metastasis research, high MEST expression was associated with poor patient survival and promoted cancer invasion and metastasis in esophageal squamous cell carcinoma (ESCC). Mechanistically, MEST activates the SRCIN1/RASAL1-ERK-snail signaling by interacting with PURA. miR-449a, a direct regulator of MEST, has its promoter hypermethylated, leading to MEST upregulation, and its mimic can suppress tumor metastasis without overt toxicity. Also, a compound G699-0288 was identified to target the MEST-PURA interaction and inhibit cancer metastasis [1].
In conclusion, Mest plays a crucial role in cancer-related biological processes, especially in promoting cancer invasion and metastasis. Research on Mest, including studies with gene-knockout models if applicable in the future, can further our understanding of its function in cancer development, potentially providing new therapeutic targets for cancer treatment.
References：
1. Xu, Wen Wen, Liao, Long, Dai, Wei, He, Qing-Yu, Li, Bin. 2023. Genome-wide CRISPR/Cas9 screening identifies a targetable MEST-PURA interaction in cancer metastasis. In EBioMedicine, 92, 104587. doi:10.1016/j.ebiom.2023.104587. https://pubmed.ncbi.nlm.nih.gov/37149929/
2. Kim, Min Soo, Lee, Hyun Sook, Kim, Yun Jae, Kang, Sung Gyun, Jin, Wook. 2019. MEST induces Twist-1-mediated EMT through STAT3 activation in breast cancers. In Cell death and differentiation, 26, 2594-2606. doi:10.1038/s41418-019-0322-9. https://pubmed.ncbi.nlm.nih.gov/30903102/
3. Zhao, Cheng, Hu, Xiheng, Tong, Shiyu, Wang, Long, Li, Yangle. . MEST promotes bladder cancer cell proliferation, migration and invasion via STAT3/Twist-1-mediated EMT. In Translational cancer research, 9, 6178-6188. doi:10.21037/tcr-20-1006. https://pubmed.ncbi.nlm.nih.gov/35117228/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen
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