C57BL/6JCya-Mestem1flox/Cya
Common Name:
Mest-flox
Product ID:
S-CKO-18537
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
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Basic Information
Strain Name
Mest-flox
Strain ID
CKOCMP-17294-Mest-B6J-VB
Gene Name
Product ID
S-CKO-18537
Gene Alias
Peg1
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
6
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Mestem1flox/Cya mice (Catalog S-CKO-18537) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000124665
NCBI RefSeq
NM_001252292.1
Target Region
Exon 4~8
Size of Effective Region
~3.0 kb
Detailed Document
Overview of Gene Research
Mest, also known as Mesoderm-specific transcript, is a gene with significant implications in multiple biological processes. It has been associated with the regulation of cell proliferation, migration, and invasion in cancer-related pathways. For example, it is involved in the IL-6/JAK/STAT3/Twist-1 signal pathway in breast cancer and the STAT3/Twist-1-mediated EMT in bladder cancer [2,3].
In cancer metastasis research, high MEST expression was associated with poor patient survival and promoted cancer invasion and metastasis in esophageal squamous cell carcinoma (ESCC). Mechanistically, MEST activates the SRCIN1/RASAL1-ERK-snail signaling by interacting with PURA. miR-449a, a direct regulator of MEST, has its promoter hypermethylated, leading to MEST upregulation, and its mimic can suppress tumor metastasis without overt toxicity. Also, a compound G699-0288 was identified to target the MEST-PURA interaction and inhibit cancer metastasis [1].
In conclusion, Mest plays a crucial role in cancer-related biological processes, especially in promoting cancer invasion and metastasis. Research on Mest, including studies with gene-knockout models if applicable in the future, can further our understanding of its function in cancer development, potentially providing new therapeutic targets for cancer treatment.
References:
1. Xu, Wen Wen, Liao, Long, Dai, Wei, He, Qing-Yu, Li, Bin. 2023. Genome-wide Nuclease technology screening identifies a targetable MEST-PURA interaction in cancer metastasis. In EBioMedicine, 92, 104587. doi:10.1016/j.ebiom.2023.104587. https://pubmed.ncbi.nlm.nih.gov/37149929/
2. Kim, Min Soo, Lee, Hyun Sook, Kim, Yun Jae, Kang, Sung Gyun, Jin, Wook. 2019. MEST induces Twist-1-mediated EMT through STAT3 activation in breast cancers. In Cell death and differentiation, 26, 2594-2606. doi:10.1038/s41418-019-0322-9. https://pubmed.ncbi.nlm.nih.gov/30903102/
3. Zhao, Cheng, Hu, Xiheng, Tong, Shiyu, Wang, Long, Li, Yangle. . MEST promotes bladder cancer cell proliferation, migration and invasion via STAT3/Twist-1-mediated EMT. In Translational cancer research, 9, 6178-6188. doi:10.21037/tcr-20-1006. https://pubmed.ncbi.nlm.nih.gov/35117228/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen