C57BL/6JCya-Dbtem1flox/Cya
Common Name:
Dbt-flox
Product ID:
S-CKO-18642
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Dbt-flox
Strain ID
CKOCMP-13171-Dbt-B6J-VB
Gene Name
Product ID
S-CKO-18642
Gene Alias
BCKAD E2; BCKAD-E2; D3Wsu60e
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
3
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Dbtem1flox/Cya mice (Catalog S-CKO-18642) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000000349
NCBI RefSeq
NM_010022
Target Region
Exon 4~5
Size of Effective Region
~2.6 kb
Detailed Document
Overview of Gene Research
Dbt, as an important kinase, plays a central role in both circadian rhythms and development [1]. It is involved in the regulation of sleep, which is subject to both circadian and homeostatic regulation [1].
In a study on Drosophila, several ethyl methanesulfonate-induced dbt mutants were investigated for their sleep patterns [1]. Using CRISPR-Cas9 to reduce DBT in all neurons expressing it led to elevated sleep during the day and reduced sleep at night [1]. Loss of sleep at night was due to dbt loss during the adult sleep/wake cycle, while increased daytime sleep was due to dbt reduction during development [1]. Targeted RNA interference showed that reduction of dbt in circadian neurons caused less sleep at night, and lower dbt expression in non-circadian neurons led to increased daytime sleep [1]. The PER protein was demonstrated to be involved in DBT-mediated sleep regulation, regardless of the type of neuron [1].
In conclusion, Dbt is a key kinase in regulating circadian rhythms and development, with a significant impact on sleep patterns. The studies on dbt mutants in Drosophila have revealed its role in sleep regulation, highlighting the importance of Dbt in maintaining normal sleep-wake cycles and providing insights into the underlying molecular mechanisms of sleep regulation [1].
References:
1. Wang, Jialin, Fan, Jin-Yuan, Zhao, Zhangwu, Dissel, Stephane, Price, Jeffrey. 2022. DBT affects sleep in both circadian and non-circadian neurons. In PLoS genetics, 18, e1010035. doi:10.1371/journal.pgen.1010035. https://pubmed.ncbi.nlm.nih.gov/35139068/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen