C57BL/6JCya-Pym1em1flox/Cya
Common Name:
Pym1-flox
Product ID:
S-CKO-18730
Background:
C57BL/6JCya
Product Type
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Genotype
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Basic Information
Strain Name
Pym1-flox
Strain ID
CKOCMP-78428-Pym1-B6J-VB
Gene Name
Product ID
S-CKO-18730
Gene Alias
A030010B05Rik; Pym; Wibg
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
10
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Pym1em1flox/Cya mice (Catalog S-CKO-18730) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000163377
NCBI RefSeq
NM_030100.4
Target Region
Exon 2~3
Size of Effective Region
~2.2 kb
Detailed Document
Overview of Gene Research
PYM1 is a protein-coding gene. It can bind both the ribosome and the RBM8A/MAGOH heterodimer of the Exon Junction Complex (EJC) core, acting as an EJC disassembly factor [1,3]. The EJC is involved in multiple post-transcriptional steps of mRNA fate modulation, and PYM1 is crucial in this process, influencing mRNA expression [1,3].
In human embryonic kidney 293 (HEK293) cells, PYM1-deficient EJCs show no defect in translation-dependent disassembly but are required for translation-independent EJC destabilization [1]. Acute reduction of PYM1 levels in HEK293 cells modestly inhibits nonsense-mediated mRNA decay and stabilizes certain mRNAs [1].
Flaviviruses, like West Nile virus, dengue virus, and Zika virus, hijack PYM1. Their capsids interact with PYM1, which is antiviral against these viruses. Flavivirus infection inhibits nonsense-mediated decay (NMD), and the capsid-PYM1 interaction interferes with EJC protein function and localization [2]. Depletion of PYM1 attenuates RBM8A binding to viral RNA, suggesting that the virus sequesters PYM1 to protect viral RNA from decay [2].
In conclusion, PYM1 is essential in regulating EJC occupancy and mRNA expression. Its role in the EJC-related pathways is crucial for normal cellular function. The interaction between PYM1 and flaviviruses reveals its significance in the host-virus interaction and the potential importance of PYM1-targeted strategies in treating flavivirus-related diseases.
References：
1. Sanjeev, Manu, Woodward, Lauren A, Schiff, Michael L, Bundschuh, Ralf, Singh, Guramrit. 2025. PYM1 limits non-canonical Exon Junction Complex occupancy in a gene architecture dependent manner to tune mRNA expression. In bioRxiv : the preprint server for biology, , . doi:10.1101/2025.03.13.643037. https://pubmed.ncbi.nlm.nih.gov/40161626/
2. Li, Minghua, Johnson, Jeffrey R, Truong, Billy, Cherry, Sara, Ramage, Holly. 2019. Identification of antiviral roles for the exon-junction complex and nonsense-mediated decay in flaviviral infection. In Nature microbiology, 4, 985-995. doi:10.1038/s41564-019-0375-z. https://pubmed.ncbi.nlm.nih.gov/30833725/
3. Gehring, Niels H, Lamprinaki, Styliani, Kulozik, Andreas E, Hentze, Matthias W. . Disassembly of exon junction complexes by PYM. In Cell, 137, 536-48. doi:10.1016/j.cell.2009.02.042. https://pubmed.ncbi.nlm.nih.gov/19410547/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen