C57BL/6JCya-Xistem1flox/Cya
Common Name:
Xist-flox
Product ID:
S-CKO-18743
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
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Basic Information
Strain Name
Xist-flox
Strain ID
CKOCMP-213742-Xist-B6J-VA
Gene Name
Product ID
S-CKO-18743
Gene Alias
A430022B11
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
X
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Xistem1flox/Cya mice (Catalog S-CKO-18743) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000127786
NCBI RefSeq
NR_001463
Target Region
Exon 2~6
Size of Effective Region
~3.1 kb
Detailed Document
Overview of Gene Research
Xist, short for X inactive-specific transcript, is a long non-coding (lnc)RNA gene. It is the master regulator of X chromosome inactivation (XCI) in mammals, a crucial dosage compensation mechanism between the sex chromosomes of males and females [1,6]. Xist RNA accumulates along the entire length of the chromosome from which it is transcribed, recruiting factors to modify chromatin and silence X-linked genes in cis [1].
In adult human B cells, XIST is continually required to silence a subset of X-linked immune genes like TLR7, through histone deacetylation and without promoter DNA methylation [2]. In naive human pluripotent stem cells (hPSCs), XIST spreads across the X chromosome, dampening X-linked gene expression, and also targets specific autosomal regions to induce repressive chromatin changes and gene expression dampening [3]. XIST has been found to play pathophysiological roles in brain tumors, being involved in processes such as migration, proliferation, angiogenesis, chemoresistance, and apoptosis evasion, especially in gliomas [4]. In NSCLC, XIST is an oncogene, abnormally upregulated, and participates in cell proliferation, invasion, migration, apoptosis, and chemosensitivity [5]. It also functions in diseases like Alzheimer's disease, where aberrations in Xist expression may be significant [7].
In conclusion, Xist is essential for X chromosome inactivation, playing a pivotal role in various biological processes and disease conditions. Studies on Xist, including those using gene-knockout models, have enhanced our understanding of its functions in diseases such as brain tumors, NSCLC, and Alzheimer's disease, providing potential insights for diagnosis, prognosis, and therapeutic strategies.
References:
1. Brockdorff, Neil, Bowness, Joseph S, Wei, Guifeng. . Progress toward understanding chromosome silencing by Xist RNA. In Genes & development, 34, 733-744. doi:10.1101/gad.337196.120. https://pubmed.ncbi.nlm.nih.gov/32482714/
2. Yu, Bingfei, Qi, Yanyan, Li, Rui, Satpathy, Ansuman T, Chang, Howard Y. 2021. B cell-specific XIST complex enforces X-inactivation and restrains atypical B cells. In Cell, 184, 1790-1803.e17. doi:10.1016/j.cell.2021.02.015. https://pubmed.ncbi.nlm.nih.gov/33735607/
3. Dror, Iris, Chitiashvili, Tsotne, Tan, Shawn Y X, Ding, Fangyuan, Plath, Kathrin. . XIST directly regulates X-linked and autosomal genes in naive human pluripotent cells. In Cell, 187, 110-129.e31. doi:10.1016/j.cell.2023.11.033. https://pubmed.ncbi.nlm.nih.gov/38181737/
4. Eldesouki, Salma, Samara, Kamel A, Qadri, Rama, Habbal, Omar, Bm Ahmed, Samrein. 2022. XIST in Brain Cancer. In Clinica chimica acta; international journal of clinical chemistry, 531, 283-290. doi:10.1016/j.cca.2022.04.993. https://pubmed.ncbi.nlm.nih.gov/35483442/
5. Shen, Yujie, Lin, Yexiang, Liu, Kai, Gao, Yisong, Yuan, Chengfu. . XIST: A Meaningful Long Noncoding RNA in NSCLC Process. In Current pharmaceutical design, 27, 1407-1417. doi:10.2174/1381612826999201202102413. https://pubmed.ncbi.nlm.nih.gov/33267757/
6. Wang, Wenlun, Min, Lu, Qiu, Xinyuan, Zhang, Dongyi, Zhu, Lingyun. 2021. Biological Function of Long Non-coding RNA (LncRNA) Xist. In Frontiers in cell and developmental biology, 9, 645647. doi:10.3389/fcell.2021.645647. https://pubmed.ncbi.nlm.nih.gov/34178980/
7. Chanda, Kaushik, Mukhopadhyay, Debashis. . LncRNA Xist, X-chromosome Instability and Alzheimer's Disease. In Current Alzheimer research, 17, 499-507. doi:10.2174/1567205017666200807185624. https://pubmed.ncbi.nlm.nih.gov/32851944/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen