C57BL/6JCya-Mark2em1flox/Cya
Common Name:
Mark2-flox
Product ID:
S-CKO-18785
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
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Basic Information
Strain Name
Mark2-flox
Strain ID
CKOCMP-13728-Mark2-B6J-VB
Gene Name
Product ID
S-CKO-18785
Gene Alias
EMK-1; Emk; Par-1; Par-1b
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
19
Phenotype
Document
Application
--
Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Mark2em1flox/Cya mice (Catalog S-CKO-18785) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000165965
NCBI RefSeq
NM_007928
Target Region
Exon 4~14
Size of Effective Region
~5.2 kb
Detailed Document
Overview of Gene Research
MARK2, also known as Microtubule-Affinity Regulating Kinase 2, is a serine/threonine protein kinase. It phosphorylates microtubule-associated proteins and is involved in multiple signaling pathways such as WNT, PI3K/AKT/mTOR, and NF-κB, playing crucial roles in cancer and the nervous system [3].
In the nervous system, MARK2 contributes to establishing neuronal polarity, developing dendritic spines, and regulating polarized vesicular transport. Mark2+/- mice showed abnormal cortical formation and partition and ASD-like behavior, suggesting MARK2 loss leads to early neuronal developmental and functional deficits via down-regulation of the WNT/β-catenin signaling pathway, thus linking it to autism spectrum disorder (ASD) [1].
In cancer, MARK2 has been studied in various contexts. For example, it is a catalytic codependency of YAP/TAZ in human cancer, and its inhibition can re-activate the Hippo tumor-suppressive pathway [2]. It also regulates chemotherapeutic responses in pancreatic cancer through the class IIa HDAC-YAP axis [4], and promotes aerobic glycolysis-mediated cell growth in breast cancer by regulating mTOR/HIF-1α and p53 pathways [5].
In conclusion, MARK2 is essential for normal neuronal development and is intricately involved in cancer-related processes. Studies using Mark2+/- mouse models have been instrumental in revealing its role in ASD, while in-vitro and in-vivo studies in cancer models have demonstrated its significance in cancer cell growth, metastasis, and chemosensitivity. This understanding of MARK2 provides potential therapeutic targets for treating ASD and certain cancers.
References:
1. Gong, Maolei, Li, Jiayi, Qin, Zailong, Liu, Chang-Mei, Chen, Xiaoli. 2024. MARK2 variants cause autism spectrum disorder via the downregulation of WNT/β-catenin signaling pathway. In American journal of human genetics, 111, 2392-2410. doi:10.1016/j.ajhg.2024.09.006. https://pubmed.ncbi.nlm.nih.gov/39419027/
2. Klingbeil, Olaf, Skopelitis, Damianos, Tonelli, Claudia, Cifani, Paolo, Vakoc, Christopher R. . MARK2/MARK3 Kinases Are Catalytic Codependencies of YAP/TAZ in Human Cancer. In Cancer discovery, 14, 2471-2488. doi:10.1158/2159-8290.CD-23-1529. https://pubmed.ncbi.nlm.nih.gov/39058094/
3. Lei, Yining, Zhang, Ruyi, Cai, Fei. 2024. Role of MARK2 in the nervous system and cancer. In Cancer gene therapy, 31, 497-506. doi:10.1038/s41417-024-00737-z. https://pubmed.ncbi.nlm.nih.gov/38302729/
4. Zeng, Yongji, Yin, Ling, Zhou, Jiuli, Chen, Yuanhong, Dong, Jixin. 2022. MARK2 regulates chemotherapeutic responses through class IIa HDAC-YAP axis in pancreatic cancer. In Oncogene, 41, 3859-3875. doi:10.1038/s41388-022-02399-3. https://pubmed.ncbi.nlm.nih.gov/35780183/
5. Ponnusamy, Lavanya, Natarajan, Sathan Raj, Manoharan, Ravi. 2022. MARK2 potentiate aerobic glycolysis-mediated cell growth in breast cancer through regulating mTOR/HIF-1α and p53 pathways. In Journal of cellular biochemistry, 123, 759-771. doi:10.1002/jcb.30219. https://pubmed.ncbi.nlm.nih.gov/35048405/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen