C57BL/6JCya-Pmf1em1flox/Cya
Common Name
Pmf1-flox
Product ID
S-CKO-18837
Backgroud
C57BL/6JCya
Strain ID
CKOCMP-67037-Pmf1-B6J-VB
When using this mouse strain in a publication, please cite “Pmf1-flox Mouse (Catalog S-CKO-18837) were purchased from Cyagen.”
Product Type
Age
Genotype
Sex
Quantity
Basic Information
Strain Name
Pmf1-flox
Strain ID
CKOCMP-67037-Pmf1-B6J-VB
Gene Name
Product ID
S-CKO-18837
Gene Alias
2600009M07Rik
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
Chr 3
Phenotype
Datasheet
Application
--
Strain Description
Ensembl Number
ENSMUST00000056370
NCBI RefSeq
NM_025928
Target Region
Exon 2~4
Size of Effective Region
~4.5 kb
Overview of Gene Research
PMF1, also known as polyamine modulated factor-1, is involved in polyamine homeostasis. Polyamines are important regulators of cell growth and death, suggesting that PMF1 may play a role in related biological processes. Additionally, in the context of plasmids, pMF1 in Myxococcus fulvus 124B02 contains genes related to plasmid maintenance and partitioning [1,3,5].
In bladder cancer, PMF1 hypermethylation is associated with gene expression loss, increasing tumor stage, and cytoplasmic PMF1 expression loss. Immunohistochemical analyses show its protein expression patterns are related to stage, grade, and overall survival. Also, PMF1 methylation in urinary specimens can distinguish bladder cancer patients from controls, indicating its potential in the clinical management of bladder cancer [2]. In a Chinese population study, the rs2984613 polymorphism in PMF1 was significantly associated with leukoaraiosis, suggesting its role in white matter lesions [4]. A pooled analysis for lacunar stroke found the SLC25A44-PMF1-BGLAP locus to be associated with cerebral white matter hyperintensities, providing insights into lacunar stroke pathogenesis [6].
In summary, PMF1 is involved in polyamine homeostasis and has implications in multiple disease areas. Studies on PMF1 in bladder cancer, leukoaraiosis, and lacunar-stroke-related white matter hyperintensities have revealed its potential role in disease progression and diagnosis. Understanding PMF1 can offer new perspectives on the underlying mechanisms of these diseases and potentially lead to novel diagnostic or therapeutic strategies.
References：
1. Li, Ya-Jie, Liu, Ya, Zhang, Zheng, Gong, Ya, Li, Yue-Zhong. 2018. A Post-segregational Killing Mechanism for Maintaining Plasmid PMF1 in Its Myxococcus fulvus Host. In Frontiers in cellular and infection microbiology, 8, 274. doi:10.3389/fcimb.2018.00274. https://pubmed.ncbi.nlm.nih.gov/30131946/
2. Aleman, Ainel, Cebrian, Virginia, Alvarez, Miguel, Esteller, Manel, Sánchez-Carbayo, Marta. . Identification of PMF1 methylation in association with bladder cancer progression. In Clinical cancer research : an official journal of the American Association for Cancer Research, 14, 8236-43. doi:10.1158/1078-0432.CCR-08-0778. https://pubmed.ncbi.nlm.nih.gov/19088041/
3. Chen, Xiao-Jing, Zhang, Zheng, Li, Ya-Jie, Sheng, Duo-Hong, Li, Yue-Zhong. . Insights into the persistence and phenotypic effects of the endogenous and cryptic plasmid pMF1 in its host strain Myxococcus fulvus 124B02. In FEMS microbiology ecology, 96, . doi:10.1093/femsec/fiaa001. https://pubmed.ncbi.nlm.nih.gov/31917409/
4. Huang, Wen-Qing, Ye, Hui-Ming, Cai, Liang-Liang, Tzeng, Chi-Meng, Lin, Qing. 2019. The Associations of PMF1, ICAM1, AGT, TRIM65, FBF1, and ACOX1 Variants With Leukoaraiosis in Chinese Population. In Frontiers in genetics, 10, 615. doi:10.3389/fgene.2019.00615. https://pubmed.ncbi.nlm.nih.gov/31396257/
5. Sun, Xia, Chen, Xiao-jing, Feng, Jing, Zhao, Jing-yi, Li, Yue-zhong. 2011. Characterization of the partitioning system of Myxococcus plasmid pMF1. In PloS one, 6, e28122. doi:10.1371/journal.pone.0028122. https://pubmed.ncbi.nlm.nih.gov/22174771/
6. Traylor, Matthew, Persyn, Elodie, Tomppo, Liisa, Lewis, Cathryn M, Markus, Hugh S. 2021. Genetic basis of lacunar stroke: a pooled analysis of individual patient data and genome-wide association studies. In The Lancet. Neurology, 20, 351-361. doi:10.1016/S1474-4422(21)00031-4. https://pubmed.ncbi.nlm.nih.gov/33773637/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen
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