C57BL/6JCya-Lats1em1flox/Cya
Common Name:
Lats1-flox
Product ID:
S-CKO-18864
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
Price:
Contact for Pricing
Basic Information
Strain Name
Lats1-flox
Strain ID
CKOCMP-16798-Lats1-B6J-VB
Gene Name
Product ID
S-CKO-18864
Gene Alias
-
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
10
Phenotype
Document
Application
--
Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Lats1em1flox/Cya mice (Catalog S-CKO-18864) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000165952
NCBI RefSeq
NM_010690
Target Region
Exon 5
Size of Effective Region
~2.0 kb
Detailed Document
Overview of Gene Research
Lats1, short for large tumor suppressor 1, is a key kinase in the Hippo signaling pathway. This pathway is evolutionarily conserved and regulates multiple biological processes such as cell growth, fate decision, organ size control, and regeneration. The Hippo pathway's core in mammals includes kinases MST1/2 and LATS1/2, along with downstream effectors YAP and TAZ, which control transcriptional programs related to cell proliferation, survival, and more [3].
In tumor-related studies, loss-of-function experiments have shown significant impacts. In murine syngeneic tumor models, deletion of LATS1/2 in tumor cells inhibits tumor growth, and this regression depends on adaptive immune responses. LATS1/2-null tumor cells secrete nucleic-acid-rich extracellular vesicles, inducing a type I interferon response, thus improving tumor immunogenicity [1]. In endometrial cancer, LATS1/2 loss promotes tumor immune evasion through down-regulating MHC-I expression, independent of the Hippo-YAP pathway. LATS1/2 directly interact with and phosphorylate STAT1, a crucial transcription factor for MHC-I upregulation in response to interferon-γ signaling [2].
In conclusion, Lats1, as part of the Hippo pathway, plays a significant role in modulating tumor immunogenicity and immune evasion in cancer. The use of gene knockout (KO) and conditional knockout (CKO) mouse models has revealed its importance in tumor-immune interactions, providing potential targets for cancer immunotherapy and highlighting its essential functions in maintaining normal biological processes related to the immune response against tumors.
References:
1. Moroishi, Toshiro, Hayashi, Tomoko, Pan, Wei-Wei, Carson, Dennis A, Guan, Kun-Liang. . The Hippo Pathway Kinases LATS1/2 Suppress Cancer Immunity. In Cell, 167, 1525-1539.e17. doi:10.1016/j.cell.2016.11.005. https://pubmed.ncbi.nlm.nih.gov/27912060/
2. Yang, Qianlan, Lv, Zehen, Wang, Mengfei, Gao, Kun, Wan, Xiaoping. 2024. LATS1/2 loss promote tumor immune evasion in endometrial cancer through downregulating MHC-I expression. In Journal of experimental & clinical cancer research : CR, 43, 54. doi:10.1186/s13046-024-02979-z. https://pubmed.ncbi.nlm.nih.gov/38383447/
3. Ma, Shenghong, Meng, Zhipeng, Chen, Rui, Guan, Kun-Liang. 2019. The Hippo Pathway: Biology and Pathophysiology. In Annual review of biochemistry, 88, 577-604. doi:10.1146/annurev-biochem-013118-111829. https://pubmed.ncbi.nlm.nih.gov/30566373/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen