C57BL/6JCya-Ros1em1flox/Cya
Common Name:
Ros1-flox
Product ID:
S-CKO-19044
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
Price:
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Basic Information
Strain Name
Ros1-flox
Strain ID
CKOCMP-19886-Ros1-B6J-VB
Gene Name
Product ID
S-CKO-19044
Gene Alias
Ros-1; c-ros
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
10
Phenotype
Document
Application
--
Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Ros1em1flox/Cya mice (Catalog S-CKO-19044) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000020045
NCBI RefSeq
NM_011282
Target Region
Exon 5
Size of Effective Region
~2.3 kb
Detailed Document
Overview of Gene Research
ROS1, a proto-oncogene, encodes a receptor tyrosine kinase [1,2,3]. Its physiological role in humans remains unknown, but it is homologous to the v-Ros sequence of University of Manchester tumours virus 2 (UR2) sarcoma virus, and its ligands are still under investigation [2]. When somatic chromosomal fusions involving ROS1 occur, chimeric oncoproteins are produced, driving various cancers in both adult and paediatric patients [1]. These oncoproteins promote cell proliferation, activation, and cell cycle progression by activating downstream signalling pathways, accelerating cancer development, especially in non-small-cell lung cancer (NSCLC) [2].
ROS1-directed tyrosine kinase inhibitors (TKIs) are effective against ROS1-fusion-positive cancers [1]. However, resistance to these TKIs can emerge in patients. Factors influencing resistance include the subcellular localization of the ROS1 oncoprotein and TKI properties [1]. Higher-affinity next-generation ROS1 TKIs have been developed to improve intracranial activity and mitigate resistance mechanisms, demonstrating clinical efficacy [1]. In NSCLC, ROS1 gene fusions are an oncogenic driver, accounting for 1%-2% of cases, and patients are initially treated with TKIs, but tumors eventually develop resistance through secondary kinase mutations or bypass signalling pathways [4,5].
In conclusion, ROS1, through its role in encoding a receptor tyrosine kinase and formation of chimeric oncoproteins upon chromosomal fusion, is significantly involved in cancer development, especially NSCLC. The study of ROS1-dependent cancers and the development of ROS1-targeted therapies, including understanding resistance mechanisms, contribute to the advancement of cancer treatment strategies for ROS1-related malignancies [1-6].
References:
1. Drilon, Alexander, Jenkins, Chelsea, Iyer, Sudarshan, Keddy, Clare, Davare, Monika A. 2020. ROS1-dependent cancers - biology, diagnostics and therapeutics. In Nature reviews. Clinical oncology, 18, 35-55. doi:10.1038/s41571-020-0408-9. https://pubmed.ncbi.nlm.nih.gov/32760015/
2. Yu, Zhi-Qiong, Wang, Meng, Zhou, Wen, Cai, Jun, Cai, Zhi-Qiang. 2022. ROS1-positive non-small cell lung cancer (NSCLC): biology, diagnostics, therapeutics and resistance. In Journal of drug targeting, 30, 845-857. doi:10.1080/1061186X.2022.2085730. https://pubmed.ncbi.nlm.nih.gov/35658765/
3. Pal, Prodipto, Khan, Zanobia. 2017. ROS1 [corrected]. In Journal of clinical pathology, 70, 1001-1009. doi:10.1136/jclinpath-2016-204244. https://pubmed.ncbi.nlm.nih.gov/28903995/
4. Giustini, Nicholas P, Bazhenova, Lyudmila. . ROS1-rearranged Non-small Cell Lung Cancer. In Thoracic surgery clinics, 30, 147-156. doi:10.1016/j.thorsurg.2020.01.007. https://pubmed.ncbi.nlm.nih.gov/32327173/
5. Boulanger, Mary C, Schneider, Jaime L, Lin, Jessica J. . Advances and future directions in ROS1 fusion-positive lung cancer. In The oncologist, 29, 943-956. doi:10.1093/oncolo/oyae205. https://pubmed.ncbi.nlm.nih.gov/39177972/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen