C57BL/6JCya-Wnt4em1flox/Cya
Common Name
Wnt4-flox
Product ID
S-CKO-19116
Backgroud
C57BL/6JCya
Strain ID
CKOCMP-22417-Wnt4-B6J-VB
When using this mouse strain in a publication, please cite “Wnt4-flox Mouse (Catalog S-CKO-19116) were purchased from Cyagen.”
Product Type
Age
Genotype
Sex
Quantity
Basic Information
Strain Name
Wnt4-flox
Strain ID
CKOCMP-22417-Wnt4-B6J-VB
Gene Name
Product ID
S-CKO-19116
Gene Alias
Wnt-4
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
Chr 4
Phenotype
Datasheet
Application
--
Strain Description
Ensembl Number
ENSMUST00000045747
NCBI RefSeq
NM_009523
Target Region
Exon 2
Size of Effective Region
~1.5 kb
Overview of Gene Research
Wnt4, a member of the WNT family of proteins, is crucial in numerous developmental pathways and tissue homeostasis. It plays a key role in the development of the female phenotype in the fetus, maintaining müllerian and reproductive tissues, and is involved in the Wnt signaling pathway [2]. It also has significance in processes like bone marrow mesenchymal stem cell (BMSC) osteogenesis and sex development [3,4].
In cardiac remodeling, conditional knockout of TEAD1 in cardiac fibroblasts (CFs) and myofibroblasts, which identified Wnt4 as a TEAD1 target, ameliorated TAC-induced cardiac remodeling, suggesting Wnt4's role in the pro-fibrotic CFs phenotype via the BRD4/Wnt4 signaling pathway [1]. In female gonadal development, Wnt4 inactivation in mice has shown its involvement in multiple steps of female reproductive development, such as Müllerian duct regression, sex-specific vasculature formation, and inhibition of steroidogenesis [5].
In conclusion, Wnt4 is essential for normal development and tissue homeostasis, especially in female-related biological processes. The use of gene knockout and conditional knockout mouse models has significantly contributed to understanding its role in diseases like cardiac remodeling and disorders in female sexual development.
References：
1. Song, Shuai, Zhang, Xiaokai, Huang, Zihang, Sun, Aijun, Ge, Junbo. 2024. TEA domain transcription factor 1(TEAD1) induces cardiac fibroblasts cells remodeling through BRD4/Wnt4 pathway. In Signal transduction and targeted therapy, 9, 45. doi:10.1038/s41392-023-01732-w. https://pubmed.ncbi.nlm.nih.gov/38374140/
2. Pitzer, Lauren M, Moroney, Marisa R, Nokoff, Natalie J, Sikora, Matthew J. . WNT4 Balances Development vs Disease in Gynecologic Tissues and Women's Health. In Endocrinology, 162, . doi:10.1210/endocr/bqab093. https://pubmed.ncbi.nlm.nih.gov/33963381/
3. Gao, Yangshuai, Zou, Yuming, Sokolowskei, Dimitri, Xu, Jiajia, Zhang, Zhongmin. 2024. Nr4a1 enhances Wnt4 transcription to promote mesenchymal stem cell osteogenesis and alleviates inflammation-inhibited bone regeneration. In Molecular therapy : the journal of the American Society of Gene Therapy, 32, 1479-1496. doi:10.1016/j.ymthe.2024.02.034. https://pubmed.ncbi.nlm.nih.gov/38429926/
4. Biason-Lauber, A, Konrad, D. 2008. WNT4 and sex development. In Sexual development : genetics, molecular biology, evolution, endocrinology, embryology, and pathology of sex determination and differentiation, 2, 210-8. doi:10.1159/000152037. https://pubmed.ncbi.nlm.nih.gov/18987495/
5. Bernard, Pascal, Harley, Vincent R. 2006. Wnt4 action in gonadal development and sex determination. In The international journal of biochemistry & cell biology, 39, 31-43. doi:. https://pubmed.ncbi.nlm.nih.gov/16905353/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen
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