C57BL/6JCya-Ltkem1flox/Cya
Common Name:
Ltk-flox
Product ID:
S-CKO-19120
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
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Basic Information
Strain Name
Ltk-flox
Strain ID
CKOCMP-17005-Ltk-B6J-VB
Gene Name
Product ID
S-CKO-19120
Gene Alias
--
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
2
Phenotype
Document
Application
--
Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Ltkem1flox/Cya mice (Catalog S-CKO-19120) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000028759
NCBI RefSeq
NM_203345
Target Region
Exon 8~11
Size of Effective Region
~2.4 kb
Detailed Document
Overview of Gene Research
Ltk, also known as leukocyte tyrosine kinase, is a receptor tyrosine kinase. It is an ER-resident receptor that regulates secretion by controlling the number of ER exit sites and ER-to-Golgi transport [4]. It also plays a role in the regulation of neuronal polarity and cortical migration by inhibiting IGF1R activity [2].
In non-small-cell lung cancer (NSCLC), the CLIP1-LTK fusion has been identified as an oncogenic driver. The kinase activity of the CLIP1-LTK fusion protein is constitutively activated and has transformation potential. Lorlatinib, an ALK inhibitor, can inhibit its kinase activity, suppress proliferation and induce apoptosis in Ba/F3 cells expressing CLIP1-LTK. However, LTK mutations, such as the L650F mutation, can lead to resistance to lorlatinib, and gilteritinib may overcome this resistance [1,3]. In Sjogren's syndrome, LTK deficiency induces macrophage M2 polarization and ameliorates the disease by reducing chemokine CXCL13 [5].
In conclusion, Ltk has diverse functions in different biological processes and disease conditions. In NSCLC, the CLIP1-LTK fusion is a targetable oncogenic driver, and understanding LTK-related resistance mechanisms is crucial for treatment. In Sjogren's syndrome, LTK is involved in macrophage polarization and disease progression, highlighting its potential as a therapeutic target in these disease areas.
References:
1. Izumi, Hiroki, Matsumoto, Shingo, Liu, Jie, Kobayashi, Susumu S, Goto, Koichi. 2021. The CLIP1-LTK fusion is an oncogenic driver in non-small-cell lung cancer. In Nature, 600, 319-323. doi:10.1038/s41586-021-04135-5. https://pubmed.ncbi.nlm.nih.gov/34819663/
2. Christova, Tania, Ho, Stephanie Ky, Liu, Ying, Gill, Mandeep, Attisano, Liliana. 2023. LTK and ALK promote neuronal polarity and cortical migration by inhibiting IGF1R activity. In EMBO reports, 24, e56937. doi:10.15252/embr.202356937. https://pubmed.ncbi.nlm.nih.gov/37291945/
3. Mori, Shunta, Izumi, Hiroki, Araki, Mitsugu, Goto, Koichi, Kobayashi, Susumu S. 2024. LTK mutations responsible for resistance to lorlatinib in non-small cell lung cancer harboring CLIP1-LTK fusion. In Communications biology, 7, 412. doi:10.1038/s42003-024-06116-6. https://pubmed.ncbi.nlm.nih.gov/38575808/
4. Centonze, Federica G, Reiterer, Veronika, Nalbach, Karsten, Behrends, Christian, Farhan, Hesso. 2019. LTK is an ER-resident receptor tyrosine kinase that regulates secretion. In The Journal of cell biology, 218, 2470-2480. doi:10.1083/jcb.201903068. https://pubmed.ncbi.nlm.nih.gov/31227593/
5. Feng, Xiuyuan, Lu, Junhui, Cheng, Wei, Chang, Xin, Wu, Jian. 2025. LTK deficiency induces macrophage M2 polarization and ameliorates Sjogren's syndrome by reducing chemokine CXCL13. In Cytokine, 190, 156905. doi:10.1016/j.cyto.2025.156905. https://pubmed.ncbi.nlm.nih.gov/40154092/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen