C57BL/6JCya-Mrtfaem1flox/Cya
Common Name:
Mrtfa-flox
Product ID:
S-CKO-19364
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
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Basic Information
Strain Name
Mrtfa-flox
Strain ID
CKOCMP-223701-Mrtfa-B6J-VB
Gene Name
Product ID
S-CKO-19364
Gene Alias
AMKL; Bsac; Mal; Mkl1; Mrtf-A
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
15
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Mrtfaem1flox/Cya mice (Catalog S-CKO-19364) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000109579
NCBI RefSeq
NM_153049
Target Region
Exon 3
Size of Effective Region
~0.8 kb
Detailed Document
Overview of Gene Research
Mrtfa, also known as Megakaryoblastic Leukemia 1 (MKL1), is a coactivator of serum response factor (SRF). It couples transcriptional regulation to actin cytoskeleton dynamics, and the MRTFA-SRF complex's transcriptional targets include cytoskeletal protein-encoding genes and immediate early genes. Mrtfa is involved in many critical cellular functions such as cell growth, apoptosis, and differentiation of various cell types including muscle and hematopoietic cells [1].
Knockout of either SRF or Mrtfa in primary hematopoietic cells decreases megakaryocyte (Mk) maturation, leading to thrombocytopenia. In HEL cells, Mrtfa overexpression enhances megakaryopoiesis by increasing SRF's genomic association and activity, and upregulating genes promoting megakaryopoiesis [2]. In cardiomyocytes, Cypher/ZASP deficiency leads to F-actin destabilization, increased G-actin levels, impaired Mrtfa nuclear localization, and abnormal cardiomyocyte maturation. Re-expression of SRF can rectify these defects [3]. In human coronary artery smooth muscle cells, Mrtfa promotes lipid accumulation, and its inhibition reduces lipid accumulation, suggesting it could be a therapeutic target for vascular lipid-related diseases [4].
In conclusion, Mrtfa is crucial in multiple biological processes. Studies using gene knockout models in hematopoietic cells, cardiomyocytes, and smooth muscle cells have revealed its roles in megakaryocyte maturation, cardiomyocyte maturation, and lipid homeostasis in the vasculature. These findings contribute to understanding the pathogenesis of related diseases and offer potential therapeutic directions.
References:
1. Reed, Fiona, Larsuel, Shannon T, Mayday, Madeline Y, Scanlon, Vanessa, Krause, Diane S. 2021. MRTFA: A critical protein in normal and malignant hematopoiesis and beyond. In The Journal of biological chemistry, 296, 100543. doi:10.1016/j.jbc.2021.100543. https://pubmed.ncbi.nlm.nih.gov/33722605/
2. Rahman, Nur-Taz, Schulz, Vincent P, Wang, Lin, Esnault, Cyril, Krause, Diane S. . MRTFA augments megakaryocyte maturation by enhancing the SRF regulatory axis. In Blood advances, 2, 2691-2703. doi:10.1182/bloodadvances.2018019448. https://pubmed.ncbi.nlm.nih.gov/30337297/
3. Lyu, Jialan, Pan, Zhicheng, Li, Ruobing, Cheng, Hongqiang, Guo, Xiaogang. 2024. Cypher/ZASP drives cardiomyocyte maturation via actin-mediated MRTFA-SRF signalling. In Theranostics, 14, 4462-4480. doi:10.7150/thno.98734. https://pubmed.ncbi.nlm.nih.gov/39113806/
4. Alajbegovic, Azra, Holmberg, Johan, Daoud, Fatima, Swärd, Karl, Albinsson, Sebastian. 2021. MRTFA overexpression promotes conversion of human coronary artery smooth muscle cells into lipid-laden foam cells. In Vascular pharmacology, 138, 106837. doi:10.1016/j.vph.2021.106837. https://pubmed.ncbi.nlm.nih.gov/33516965/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen