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C57BL/6JCya-Eef1akmt3em1/Cya
Common Name:
Eef1akmt3-KO
Product ID:
S-KO-00153
Background:
C57BL/6JCya
Product Type
Age
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Sex
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Basic Information
Strain Name
Eef1akmt3-KO
Strain ID
KOCMP-100504608-Eef1akmt3-B6J-VA
Gene Name
Eef1akmt3
Product ID
S-KO-00153
Gene Alias
EG546486; Fam119b; Gm16109; Mettl21b
Background
C57BL/6JCya
NCBI ID
100504608
Modification
Conventional knockout
Chromosome
10
Phenotype
MGI:3645330
Document
Click here to download >>
Application
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Rare Disease Data Center >>
Note
Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Eef1akmt3em1/Cya mice (Catalog S-KO-00153) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000116231
NCBI RefSeq
NM_001205036
Target Region
Exon 3
Size of Effective Region
~1.2 kb
Detailed Document
Click here to download >>
Overview of Gene Research
Eef1akmt3, also known as METTL21B, is a lysine methyltransferase. It specifically targets and methylates eukaryotic translation elongation factor 1 alpha (eEF1A) at Lys-165, affecting mRNA translation [3,4,5]. It is involved in multiple biological processes, and its dysregulation is associated with diseases such as cancer [1,3]. Gene knockout models can be valuable for studying its functions in vivo.

In gastric cancer, Eef1akmt3 expression was lower in cancer tissues, and its knockdown increased tumor invasiveness and migration. Eef1akmt3 was identified as a tumor-suppressive methyltransferase that catalyzes the monomethylation of MAP2K7 at K296, reducing the phosphorylation, ubiquitination, and degradation of TP53. This reveals the tumor-suppressive function of the Eef1akmt3/MAP2K7/TP53 signaling axis, suggesting it as a potential targeted therapy for gastric cancer [1]. In endothelial cells, RIPK1 phosphorylates Eef1akmt3 at Ser26, inhibiting its methyltransferase activity and global protein synthesis, which attenuates N-Shh translation and secretion. Endothelial RIPK1 knockout exacerbated graft stenosis, and treatment with a Hedgehog pathway inhibitor alleviated this, indicating a protective role of endothelial RIPK1-regulated Eef1akmt3 in arterial bypass graft vascular remodeling [2].

In conclusion, Eef1akmt3 is a significant methyltransferase involved in mRNA translation and protein methylation. Through gene-knockout-based research, its role in suppressing tumor invasiveness and metastasis in gastric cancer and in protecting artery bypass grafts from arteriosclerosis has been revealed. These findings contribute to understanding the underlying mechanisms of related diseases and may provide new therapeutic strategies.

References:

1. Hong, Yo Han, Aziz, Nur, Park, Jae Gwang, Hur, Hoon, Cho, Jae Youl. 2022. The EEF1AKMT3/MAP2K7/TP53 axis suppresses tumor invasiveness and metastasis in gastric cancer. In Cancer letters, 544, 215803. doi:10.1016/j.canlet.2022.215803. https://pubmed.ncbi.nlm.nih.gov/35753528/

2. Lu, Yao, Leng, Yiming, Li, Yalan, Weng, Liang, Xu, Qingbo. 2023. Endothelial RIPK1 protects artery bypass graft against arteriosclerosis by regulating SMC growth. In Science advances, 9, eadh8939. doi:10.1126/sciadv.adh8939. https://pubmed.ncbi.nlm.nih.gov/37647392/

3. Shu, Xin, Li, Xinquan, Xiang, Xiaochen, Wang, Qiang, Wu, Qingming. 2021. METTL21B is a prognostic biomarker and potential therapeutic target in low-grade gliomas. In Aging, 13, 20661-20683. doi:10.18632/aging.203454. https://pubmed.ncbi.nlm.nih.gov/34446611/

4. Hamey, Joshua J, Wienert, Beeke, Quinlan, Kate G R, Wilkins, Marc R. 2017. METTL21B Is a Novel Human Lysine Methyltransferase of Translation Elongation Factor 1A: Discovery by Nuclease technology Knockout. In Molecular & cellular proteomics : MCP, 16, 2229-2242. doi:10.1074/mcp.M116.066308. https://pubmed.ncbi.nlm.nih.gov/28663172/

5. Malecki, Jedrzej, Aileni, Vinay Kumar, Ho, Angela Y Y, Leidel, Sebastian A, Falnes, Pål Ø. . The novel lysine specific methyltransferase METTL21B affects mRNA translation through inducible and dynamic methylation of Lys-165 in human eukaryotic elongation factor 1 alpha (eEF1A). In Nucleic acids research, 45, 4370-4389. doi:10.1093/nar/gkx002. https://pubmed.ncbi.nlm.nih.gov/28108655/

Quality Control Standard
Sperm Test

Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.

Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.

Environmental Standards:SPF
Available Region:Global
Source:Cyagen
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