C57BL/6JCya-Dekem1/Cya
Common Name
Dek-KO
Product ID
S-KO-00728
Backgroud
C57BL/6JCya
Strain ID
KOCMP-110052-Dek-B6J-VA
When using this mouse strain in a publication, please cite “Dek-KO Mouse (Catalog S-KO-00728) were purchased from Cyagen.”
Product Type
Age
Genotype
Sex
Quantity
Basic Information
Strain Name
Dek-KO
Strain ID
KOCMP-110052-Dek-B6J-VA
Gene Name
Product ID
S-KO-00728
Gene Alias
D13H6S231E, 1810019E15Rik
Background
C57BL/6JCya
NCBI ID
Modification
Conventional knockout
Chromosome
Chr 13
Phenotype
Datasheet
Application
--
Strain Description
Ensembl Number
ENSMUST00000021807
NCBI RefSeq
NM_025900
Target Region
Exon 3~7
Size of Effective Region
~3.9 kb
Overview of Gene Research
DEK, an oncogene, is a highly conserved nuclear factor [2,4]. It plays important roles in regulating multiple cellular processes, such as epigenetic and transcriptional regulation [4]. DEK is also involved in cellular transformation, affecting processes like self-renewal, proliferation, and apoptosis [4]. The secreted form of DEK can regulate hematopoiesis by activating signaling through the CXCL2 receptor, which impacts the proliferation of hematopoietic stem cells and the number of hematopoietic progenitor cells [3].
In hematopoietic stem cells (HSCs), nuclear DEK is crucial. HSCs lacking DEK show defects in long-term self-renew capacity, leading to impaired hematopoiesis [1]. DEK deficiency reduces quiescence and accelerates mitochondrial metabolism in HSCs, in part by activating mTOR signaling [1]. At the molecular level, DEK recruits the corepressor NCoR1 to repress histone 3 acetylation at lysine 27 (H3K27ac), restricting chromatin accessibility and governing the expression of quiescence-associated genes [1].
In conclusion, DEK is essential for maintaining normal cellular functions, especially in hematopoiesis. The study of DEK-deficient mouse models has revealed its key role in preserving HSC potential, uncovering a link between chromatin remodelers and HSC homeostasis, which has important implications for understanding and potentially treating hematopoietic-related diseases [1].
References:
1. Chen, Zhe, Huo, Dawei, Li, Lei, Chen, Jieping, Hou, Yu. . Nuclear DEK preserves hematopoietic stem cells potential via NCoR1/HDAC3-Akt1/2-mTOR axis. In The Journal of experimental medicine, 218, . doi:10.1084/jem.20201974. https://pubmed.ncbi.nlm.nih.gov/33755722/
2. Ishida, Kazuhisa, Nakashima, Takayuki, Shibata, Toshiyuki, Hara, Akira, Tomita, Hiroyuki. 2020. Role of the DEK oncogene in the development of squamous cell carcinoma. In International journal of clinical oncology, 25, 1563-1569. doi:10.1007/s10147-020-01735-5. https://pubmed.ncbi.nlm.nih.gov/32656741/
3. Bodine, David M. 2019. All hands on DEK. In The Journal of clinical investigation, 129, 2205-2206. doi:10.1172/JCI128986. https://pubmed.ncbi.nlm.nih.gov/31107244/
4. Sandén, C, Gullberg, U. 2015. The DEK oncoprotein and its emerging roles in gene regulation. In Leukemia, 29, 1632-6. doi:10.1038/leu.2015.72. https://pubmed.ncbi.nlm.nih.gov/25765544/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen
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