C57BL/6NCya-Chrna7em1/Cya
Common Name:
Chrna7-KO
Product ID:
S-KO-00855
Background:
C57BL/6NCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Chrna7-KO
Strain ID
KOCMP-11441-Chrna7-B6N-VA
Gene Name
Product ID
S-KO-00855
Gene Alias
Acra7; alpha7; nAchR
Background
C57BL/6NCya
NCBI ID
Modification
Conventional knockout
Chromosome
7
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6NCya-Chrna7em1/Cya mice (Catalog S-KO-00855) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000032738
NCBI RefSeq
NM_007390
Target Region
Exon 5~9
Size of Effective Region
~6.7 kb
Detailed Document
Overview of Gene Research
CHRNA7, encoding the α7 neuronal nicotinic acetylcholine receptor, is ubiquitously expressed in the central nervous system and periphery. It is involved in cholinergic neurotransmission, and its function is crucial for normal cognitive processes. The regulation of CHRNA7 is complex, with a partial duplication creating the CHRFAM7A gene that can dominantly negatively regulate CHRNA7 function [1].
Chrna7 deficient mice have been studied to understand its role in diseases. However, these mice did not show consistent neuropsychiatric and behavioral phenotypes related to human conditions like schizophrenia, bipolar disorder, epilepsy, etc. [4]. In contrast, CHRNA7 copy number variants in humans are associated with multiple neuropsychiatric conditions. Deletions are linked to more severe phenotypes, such as in Rett syndrome, and are enriched in risperidone-treated children and adolescents with severe aggression [3,5]. Gains are relatively prevalent among adolescents with major depressive and anxiety disorders [6]. Also, mutations in RIC3, a chaperone of CHRNA7, provide evidence for the role of the cholinergic pathway in Parkinson's disease [2].
In summary, CHRNA7 is vital for cholinergic neurotransmission and normal cognitive function. While Chrna7 knockout mouse models have not fully replicated human disease phenotypes, studies on human CHRNA7 copy number variants and associated chaperone mutations have revealed its significant role in various neuropsychiatric and neurodegenerative diseases.
References:
1. Sinkus, Melissa L, Graw, Sharon, Freedman, Robert, Lester, Henry A, Leonard, Sherry. 2015. The human CHRNA7 and CHRFAM7A genes: A review of the genetics, regulation, and function. In Neuropharmacology, 96, 274-88. doi:10.1016/j.neuropharm.2015.02.006. https://pubmed.ncbi.nlm.nih.gov/25701707/
2. Cherian, Ajith, K P, Divya, Vijayaraghavan, Asish. 2023. Parkinson's disease - genetic cause. In Current opinion in neurology, 36, 292-301. doi:10.1097/WCO.0000000000001167. https://pubmed.ncbi.nlm.nih.gov/37366140/
3. Gillentine, Madelyn A, Schaaf, Christian P. 2015. The human clinical phenotypes of altered CHRNA7 copy number. In Biochemical pharmacology, 97, 352-362. doi:10.1016/j.bcp.2015.06.012. https://pubmed.ncbi.nlm.nih.gov/26095975/
4. Yin, Jiani, Chen, Wu, Yang, Hongxing, Xue, Mingshan, Schaaf, Christian P. 2017. Chrna7 deficient mice manifest no consistent neuropsychiatric and behavioral phenotypes. In Scientific reports, 7, 39941. doi:10.1038/srep39941. https://pubmed.ncbi.nlm.nih.gov/28045139/
5. Gillentine, Madelyn A, White, Janson J, Grochowski, Christopher M, Schaaf, Christian P, Calarge, Chadi A. 2017. CHRNA7 Deletions are Enriched in Risperidone-Treated Children and Adolescents. In Journal of child and adolescent psychopharmacology, 27, 908-915. doi:10.1089/cap.2017.0068. https://pubmed.ncbi.nlm.nih.gov/28817303/
6. Gillentine, Madelyn A, Lozoya, Ricardo, Yin, Jiani, Schaaf, Christian P, Calarge, Chadi A. 2018. CHRNA7 copy number gains are enriched in adolescents with major depressive and anxiety disorders. In Journal of affective disorders, 239, 247-252. doi:10.1016/j.jad.2018.07.017. https://pubmed.ncbi.nlm.nih.gov/30029151/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen