C57BL/6JCya-Gria1em1/Cya
Common Name:
Gria1-KO
Product ID:
S-KO-02332
Background:
C57BL/6JCya
Product Type
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Genotype
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Basic Information
Strain Name
Gria1-KO
Strain ID
KOCMP-14799-Gria1-B6J-VA
Gene Name
Product ID
S-KO-02332
Gene Alias
2900051M01Rik; Glr-1; Glr1; GluA1; GluR-A; GluRA; Glur-1; Glur1; HIPA1; gluR-K1
Background
C57BL/6JCya
NCBI ID
Modification
Conventional knockout
Chromosome
11
Phenotype
Document
Application
--
Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Gria1em1/Cya mice (Catalog S-KO-02332) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000036315
NCBI RefSeq
NM_001113325
Target Region
Exon 4
Size of Effective Region
~1.5 kb
Detailed Document
Overview of Gene Research
GRIA1, encoding the GluA1 subunit of α-amino-3-hydroxy-5-methyl-4-isoxazole propionate (AMPA) receptors, is a crucial gene. AMPA receptors are ligand-gated ion channels that act as excitatory receptors for the neurotransmitter L-glutamate, playing a vital role in excitatory neurotransmission in the brain [1,3].
Functional studies on individuals with GRIA1 variants, including those with de novo missense and nonsense variants, have shown that these can lead to a neurodevelopmental disorder mostly affecting cognition and speech [3]. The p.(Ala636Thr) variant, for example, is recurrent and is associated with motor and/or language delay, intellectual disability, behavioral and psychiatric comorbidities, hypotonia and epilepsy [1]. In addition, Xenopus gria1 CRISPR-Cas9 F0 models, a form of in vivo study, showed transient motor deficits, an intermittent seizure phenotype, and significant working memory impairment in mutants, indicating the importance of GRIA1 in these functions [3]. Sevoflurane preconditioning, which downregulates GRIA1 expression, can attenuate cerebral ischemia-reperfusion-induced neuronal injury, suggesting GRIA1's role in this pathological process [2].
In conclusion, GRIA1 is essential for normal brain function, especially in excitatory neurotransmission. Research using genetic models like the Xenopus gria1 models has revealed its role in neurodevelopmental disorders and neurological injury. Understanding GRIA1 can provide insights into the mechanisms of these diseases and potentially lead to new therapeutic strategies.
References:
1. Tvergaard, Nicolai Kohring, Tkemaladze, Tinatin, Stödberg, Tommy, Tümer, Zeynep, Bayat, Allan. 2024. Unraveling GRIA1 neurodevelopmental disorders: Lessons learned from the p.(Ala636Thr) variant. In Clinical genetics, 106, 427-436. doi:10.1111/cge.14577. https://pubmed.ncbi.nlm.nih.gov/38890806/
2. Li, Ye, Liang, Zhi, Lei, Shuyan, Ma, Kai, Chi, Kui. 2023. Sevoflurane Preconditioning Downregulates GRIA1 Expression to Attenuate Cerebral Ischemia-Reperfusion-Induced Neuronal Injury. In Neurotoxicity research, 41, 29-40. doi:10.1007/s12640-022-00620-5. https://pubmed.ncbi.nlm.nih.gov/36595163/
3. Ismail, Vardha, Zachariassen, Linda G, Godwin, Annie, Kristensen, Anders S, Baralle, Diana. 2022. Identification and functional evaluation of GRIA1 missense and truncation variants in individuals with ID: An emerging neurodevelopmental syndrome. In American journal of human genetics, 109, 1217-1241. doi:10.1016/j.ajhg.2022.05.009. https://pubmed.ncbi.nlm.nih.gov/35675825/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen