Logo
Homepage
Explore Our Models
My Cart
Contact
Subscribe
Models
Genetically Engineered Animals
Knockout Mice
Knockout Rats
Knockin Mice
Knockin Rats
Transgenic Mice
Transgenic Rats
Model Generation Techniques
Turboknockout<sup>®</sup> Gene Targeting
ES Cell Gene Targeting
Targeted Gene Editing
Regular Transgenic
PiggyBac Transgenesis
BAC Transgenic
Research Models
HUGO-GT™ Humanized Mice
Cre Mouse Lines
Humanized Target Gene Models
Metabolic Disease Models
Ophthalmic Disease Models
Neurological Disease Models
Autoimmune Disease Models
Immunodeficient Mouse Models
Humanized Immune System Mouse Models
Oncology & Immuno-oncology Models
Covid-19 Mouse Models
MouseAtlas Model Library
Knockout Cell Line Product Catalog
Tumor Cell Line Product Catalog
AAV Standard Product Catalog
Animal Supporting Services
Breeding Services
Cryopreservation & Recovery
Phenotyping Services
BAC Modification
Custom Cell Line Models
Induced Pluripotent Stem Cells (iPSCs)
Knockout Cell Lines
Knockin Cell Lines
Point Mutation Cell Lines
Overexpression Cell Lines
Virus Packaging
Adeno-associated Virus (AAV) Packaging
Lentivirus Packaging
Adenovirus Packaging
CRO Services
By Therapeutic Area
Oncology
Ophthalmology
Neuroscience
Metabolic & Cardiovascular Diseases
Autoimmune & Inflammatory
By Drug Type
AI-Powered AAV Discovery
Gene Therapy
Oligonucleotide Therapy
Antibody Therapy
Cell Immunotherapy
Resources
Promotion
Events & Webinars
Newsroom
Blogs & Insights
Resource Vault
Reference Databases
Peer-Reviewed Citations
Rare Disease Data Center
AbSeek
Cell iGeneEditor™ System
OriCell
Quality
Facility Overview
Animal Health & Welfare
Health Reports
About Us
Corporate Overview
Our Partners
Careers
Contact Us
Login
Request a Product Quote
Select products from our catalogs and submit your request. Our team will get back to you with detailed information.
Full Name
Email
Phone Number
Organization
Job Role
Country
Catalog Type
Product Name
Additional Comments
Cyagen values your privacy. We’d like to keep you informed about our latest offerings and insights. Your preferences:
You may unsubscribe from these communications at any time. See our Privacy Policy for details on opting out and data protection.
By clicking the button below, you consent to allow Cyagen to store and process the personal information submitted in this form to provide you the content requested.
C57BL/6NCya-Mettl1em1/Cya
Common Name:
Mettl1-KO
Product ID:
S-KO-03182
Background:
C57BL/6NCya
Product Type
Age
Genotype
Sex
Quantity
Price:
Contact for Pricing
Basic Information
Strain Name
Mettl1-KO
Strain ID
KOCMP-17299-Mettl1-B6N-VB
Gene Name
Mettl1
Product ID
S-KO-03182
Gene Alias
2810012D02Rik
Background
C57BL/6NCya
NCBI ID
17299
Modification
Conventional knockout
Chromosome
10
Phenotype
MGI:1339986
Document
Click here to download >>
Application
--
More
Rare Disease Data Center >>
Note
Note: When using this mouse strain in a publication, please cite “C57BL/6NCya-Mettl1em1/Cya mice (Catalog S-KO-03182) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000006915
NCBI RefSeq
NM_010792
Target Region
Exon 3~6
Size of Effective Region
~0.8 kb
Detailed Document
Click here to download >>
Overview of Gene Research
METTL1, short for methyltransferase-like protein-1, is a key component of the tRNA N7-methylguanosine (m7G) methyltransferase complex along with WDR4. It catalyzes the m7G modification of tRNAs, which is crucial for tRNA stability and function, and is involved in various biological processes such as translation [4,5,7].

In cancer research, METTL1 has been found to play oncogenic roles. Depletion of METTL1 in cancer cells leads to decreased abundance of m7G-modified tRNAs, altered cell cycle, and inhibited oncogenicity. For instance, in hepatocellular carcinoma, knockdown of METTL1 overrode lenvatinib resistance by impairing cell proliferation and promoting apoptosis [1]. In bladder cancer, silencing METTL1 suppressed cell proliferation, migration, and invasion both in vitro and in vivo [2]. In prostate cancer, knockdown of Mettl1 in pre-clinical models increased intratumoural infiltration of pro-inflammatory immune cells and enhanced responses to immunotherapy [6]. Also, in post-radiofrequency ablation recurrent hepatocellular carcinoma, METTL1 expression was enhanced, accompanied by increased PMN-MDSCs and decreased CD8+ T cells, and interrupting the METTL1-TGF-β2-PMN-MDSC axis could prevent recurrence [3].

In conclusion, METTL1-mediated m7G tRNA modification is essential for normal biological functions, and its dysregulation is closely associated with cancer development and drug resistance. The use of gene knockout or knockdown models in these studies has revealed METTL1's critical role in promoting tumorigenesis, immunosuppressive microenvironment formation, and drug resistance in various cancers, providing potential therapeutic targets for cancer treatment.

References:

1. Huang, Manling, Long, Jianting, Yao, Zhijia, Xu, Lixia, Kuang, Ming. . METTL1-Mediated m7G tRNA Modification Promotes Lenvatinib Resistance in Hepatocellular Carcinoma. In Cancer research, 83, 89-102. doi:10.1158/0008-5472.CAN-22-0963. https://pubmed.ncbi.nlm.nih.gov/36102722/

2. Ying, Xiaoling, Liu, Bixia, Yuan, Zusen, Luo, Junhang, Ji, Weidong. . METTL1-m7 G-EGFR/EFEMP1 axis promotes the bladder cancer development. In Clinical and translational medicine, 11, e675. doi:10.1002/ctm2.675. https://pubmed.ncbi.nlm.nih.gov/34936728/

3. Zeng, Xuezhen, Liao, Guanrui, Li, Shumin, Chen, Shuling, Kuang, Ming. 2022. Eliminating METTL1-mediated accumulation of PMN-MDSCs prevents hepatocellular carcinoma recurrence after radiofrequency ablation. In Hepatology (Baltimore, Md.), 77, 1122-1138. doi:10.1002/hep.32585. https://pubmed.ncbi.nlm.nih.gov/35598182/

4. Ruiz-Arroyo, Victor M, Raj, Rishi, Babu, Kesavan, Roberts, Paul H, Nam, Yunsun. 2023. Structures and mechanisms of tRNA methylation by METTL1-WDR4. In Nature, 613, 383-390. doi:10.1038/s41586-022-05565-5. https://pubmed.ncbi.nlm.nih.gov/36599982/

5. Cheng, Wenli, Gao, Aili, Lin, Hui, Zhang, Wenjuan. 2022. Novel roles of METTL1/WDR4 in tumor via m7G methylation. In Molecular therapy oncolytics, 26, 27-34. doi:10.1016/j.omto.2022.05.009. https://pubmed.ncbi.nlm.nih.gov/35784404/

6. García-Vílchez, Raquel, Añazco-Guenkova, Ana M, Dietmann, Sabine, Carracedo, Arkaitz, Blanco, Sandra. 2023. METTL1 promotes tumorigenesis through tRNA-derived fragment biogenesis in prostate cancer. In Molecular cancer, 22, 119. doi:10.1186/s12943-023-01809-8. https://pubmed.ncbi.nlm.nih.gov/37516825/

7. Li, Jiazhi, Wang, Longfei, Hahn, Quentin, Fischer, Eric S, Gregory, Richard I. 2023. Structural basis of regulated m7G tRNA modification by METTL1-WDR4. In Nature, 613, 391-397. doi:10.1038/s41586-022-05566-4. https://pubmed.ncbi.nlm.nih.gov/36599985/

Quality Control Standard
Sperm Test

Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.

Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.

Environmental Standards:SPF
Available Region:Global
Source:Cyagen
Model Library
Model Library
Resources
Resources
Animal Quality
Animal Quality
Get Support
Get Support
Address:
2255 Martin Avenue, Suite E Santa Clara, CA 95050-2709, US
Tel:
800-921-8930 (8-6pm PST)
+1408-963-0306 (lnt’l)
Fax:
408-969-0338
Email:
animal-service@cyagen.com
service@cyagen.us
CRO Services
OncologyOphthalmologyNeuroscienceMetabolic & CardiovascularAutoimmune & InflammatoryGene TherapyAntibody Therapy
About Us
Corporate OverviewOur PartnersCareersContact Us
Social Media
Disclaimer: Pricing and availability of our products and services vary by region. Listed prices are applicable to the specific countries. Please contact us for more information.
Copyright © 2025 Cyagen. All rights reserved.
Privacy Policy
Site Map
Stay Updated with the Latest from Cyagen
Get the latest news on our research models, CRO services, scientific resources, and special offers—tailored to your research needs and delivered straight to your inbox.
Full Name
Email
Organization
Country
Areas of Interest