C57BL/6JCya-Nmt1em1/Cya
Common Name:
Nmt1-KO
Product ID:
S-KO-03392
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Nmt1-KO
Strain ID
KOCMP-18107-Nmt1-B6J-VA
Gene Name
Product ID
S-KO-03392
Gene Alias
-
Background
C57BL/6JCya
NCBI ID
Modification
Conventional knockout
Chromosome
11
Phenotype
Document
Application
--
Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Nmt1em1/Cya mice (Catalog S-KO-03392) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000021314
NCBI RefSeq
NM_008707
Target Region
Exon 4
Size of Effective Region
~1.5 kb
Detailed Document
Overview of Gene Research
Nmt1, short for N-myristoyltransferase 1, is an essential eukaryotic enzyme. It catalyzes the transfer of myristoyl groups to the amino-terminal residues of numerous proteins. This process is crucial for the growth and development of many eukaryotes and viruses. N-myristoylation, the reaction it mediates, is involved in various cellular processes such as signal transduction, cellular transformation, and tumorigenesis [4].
In cancer research, multiple studies have demonstrated the significance of Nmt1. In hepatocellular carcinoma (HCC), upregulation of Nmt1 expression enhanced tumor growth, while Nmt1 knockdown suppressed it both in vitro and in vivo. Nmt1 was found to myristoylate VILIP3 protein, and high Nmt1 or VILIP3 expression was associated with advanced stages and poor survival in HCC. Desloratadine, an anti-allergic drug, binds to Nmt1 and inhibits its enzymatic activity, disrupting the Nmt1-mediated myristoylation of VILIP3 protein and subsequent NFκB/Bcl-2 signaling [1]. In breast cancer, genetic inhibition of Nmt1 suppressed the initiation, proliferation, and invasion of breast cancer cells in vitro and in vivo. Nmt1 knockdown promoted oxidative stress, which along with endoplasmic reticulum (ER) stress, activated the JNK pathway leading to autophagy and abrogating breast cancer progression, especially in triple-negative breast cancer (TNBC) [3]. In non-small cell lung cancer (NSCLC), Nmt1 was highly expressed in spheroid cells and facilitated the stemness of NSCLC cells by activating the PI3K/AKT pathway. It also accelerated NSCLC tumor metastasis and resistance to cisplatin [2]. In liver cancer, Nmt1-mediated N-myristoylation differentially regulated the ubiquitination of target proteins. It suppressed anti-tumorigenic proteins and stimulated pro-tumorigenic proteins, resulting in a pro-tumorigenic outcome [5].
In conclusion, Nmt1 is crucial for the N-myristoylation of proteins, which is involved in multiple biological processes. Through gene knockout or knockdown models in various cancer types, Nmt1 has been shown to play significant roles in tumor development, metastasis, and drug resistance. These findings suggest that Nmt1 could be a potential biomarker and therapeutic target for treating cancers such as HCC, breast cancer, NSCLC, and liver cancer.
References:
1. Tan, Xiang-Peng, He, Yan, Yang, Jing, Guan, Xin-Yuan, Li, Bin. 2023. Blockade of NMT1 enzymatic activity inhibits N-myristoylation of VILIP3 protein and suppresses liver cancer progression. In Signal transduction and targeted therapy, 8, 14. doi:10.1038/s41392-022-01248-9. https://pubmed.ncbi.nlm.nih.gov/36617552/
2. Zou, Wailong, Zhang, Xinjun, Wang, Yumin, Zhang, Jia, Chen, Jichao. 2022. NMT1 Enhances the Stemness of NSCLC Cells by Activating the PI3K/AKT Pathway. In Pharmacology, 107, 486-494. doi:10.1159/000525095. https://pubmed.ncbi.nlm.nih.gov/35732157/
3. Deng, Lu, Gao, Xinlei, Liu, Bingjie, Wu, Qingfa, Liu, Suling. 2018. NMT1 inhibition modulates breast cancer progression through stress-triggered JNK pathway. In Cell death & disease, 9, 1143. doi:10.1038/s41419-018-1201-x. https://pubmed.ncbi.nlm.nih.gov/30446635/
4. Wang, Hong, Xu, Xin, Wang, Jiayi, Qiao, Yongxia. . The role of N-myristoyltransferase 1 in tumour development. In Annals of medicine, 55, 1422-1430. doi:10.1080/07853890.2023.2193425. https://pubmed.ncbi.nlm.nih.gov/37140999/
5. Zhu, Guoqing, Wang, Feng, Li, Haojie, Qiao, Yongxia, Pan, Qiuhui. 2021. N-Myristoylation by NMT1 Is POTEE-Dependent to Stimulate Liver Tumorigenesis via Differentially Regulating Ubiquitination of Targets. In Frontiers in oncology, 11, 681366. doi:10.3389/fonc.2021.681366. https://pubmed.ncbi.nlm.nih.gov/34136404/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen