Logo
Homepage
Explore Our Models
My Cart
Contact
Subscribe
Models
Genetically Engineered Animals
Knockout Mice
Knockout Rats
Knockin Mice
Knockin Rats
Transgenic Mice
Transgenic Rats
Model Generation Techniques
Turboknockout<sup>®</sup> Gene Targeting
ES Cell Gene Targeting
Targeted Gene Editing
Regular Transgenic
PiggyBac Transgenesis
BAC Transgenic
Research Models
HUGO-GT™ Humanized Mice
Cre Mouse Lines
Humanized Target Gene Models
Metabolic Disease Models
Ophthalmic Disease Models
Neurological Disease Models
Autoimmune Disease Models
Immunodeficient Mouse Models
Humanized Immune System Mouse Models
Oncology & Immuno-oncology Models
Covid-19 Mouse Models
MouseAtlas Model Library
Knockout Cell Line Product Catalog
Tumor Cell Line Product Catalog
AAV Standard Product Catalog
Animal Supporting Services
Breeding Services
Cryopreservation & Recovery
Phenotyping Services
BAC Modification
Custom Cell Line Models
Induced Pluripotent Stem Cells (iPSCs)
Knockout Cell Lines
Knockin Cell Lines
Point Mutation Cell Lines
Overexpression Cell Lines
Virus Packaging
Adeno-associated Virus (AAV) Packaging
Lentivirus Packaging
Adenovirus Packaging
CRO Services
By Therapeutic Area
Oncology
Ophthalmology
Neuroscience
Metabolic & Cardiovascular Diseases
Autoimmune & Inflammatory
By Drug Type
AI-Powered AAV Discovery
Gene Therapy
Oligonucleotide Therapy
Antibody Therapy
Cell Immunotherapy
Resources
Promotion
Events & Webinars
Newsroom
Blogs & Insights
Resource Vault
Reference Databases
Peer-Reviewed Citations
Rare Disease Data Center
AbSeek
Cell iGeneEditor™ System
OriCell
Quality
Facility Overview
Animal Health & Welfare
Health Reports
About Us
Corporate Overview
Our Partners
Careers
Contact Us
Login
Request a Product Quote
Select products from our catalogs and submit your request. Our team will get back to you with detailed information.
Full Name
Email
Phone Number
Organization
Job Role
Country
Catalog Type
Product Name
Additional Comments
Cyagen values your privacy. We’d like to keep you informed about our latest offerings and insights. Your preferences:
You may unsubscribe from these communications at any time. See our Privacy Policy for details on opting out and data protection.
By clicking the button below, you consent to allow Cyagen to store and process the personal information submitted in this form to provide you the content requested.
C57BL/6NCya-Chd3em1/Cya
Common Name:
Chd3-KO
Product ID:
S-KO-05222
Background:
C57BL/6NCya
Product Type
Age
Genotype
Sex
Quantity
Price:
Contact for Pricing
Basic Information
Strain Name
Chd3-KO
Strain ID
KOCMP-216848-Chd3-B6N-VA
Gene Name
Chd3
Product ID
S-KO-05222
Gene Alias
2600010P09Rik; Chd7; Prp7; Prp9-1
Background
C57BL/6NCya
NCBI ID
216848
Modification
Conventional knockout
Chromosome
11
Phenotype
MGI:1344395
Document
Click here to download >>
Application
--
More
Rare Disease Data Center >>
Note
Note: When using this mouse strain in a publication, please cite “C57BL/6NCya-Chd3em1/Cya mice (Catalog S-KO-05222) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000108661
NCBI RefSeq
NM_146019
Target Region
Exon 2~11
Size of Effective Region
~5.0 kb
Detailed Document
Click here to download >>
Overview of Gene Research
CHD3, or Chromodomain Helicase DNA Binding Protein 3, is a key component in chromatin remodeling, being involved in the NuRD complex (nucleosome remodeling and deacetylase activities). It functions in modulating chromatin structure and gene expression by deacetylating histones, which is crucial for various biological processes [3,4].

Mutations in CHD3 are associated with Snijders Blok-Campeau syndrome, an autosomal-dominant neurodevelopmental disorder. Probands with inherited CHD3 variants show phenotypes overlapping those with de novo variants, including global developmental delay, intellectual disability, speech and language difficulties, behavioral disorders, and typical dysmorphic features, while heterozygote parents may be mildly or not affected, indicating variable expressivity [1,3,5,7,8]. Computational and experimental studies have shown that mutations in the ATPase/helicase domain of CHD3 can disrupt critical binding and interaction motifs, affecting ATPase activity and chromatin remodeling [2]. Generation of Chd3-floxed allele in mice showed that while CHD3 is dispensable for early vascular development, global deletion of Chd3 led to partial lethality of Chd3Δ/Δ mutants prior to weaning, suggesting its role in embryonic viability [6].

In conclusion, CHD3 is essential for chromatin remodeling and plays a significant role in embryonic viability. Its mutations are strongly associated with Snijders Blok-Campeau syndrome, highlighting the importance of CHD3 functional studies, especially those using mouse models, in understanding neurodevelopmental disorders.

References:

1. van der Spek, Jet, den Hoed, Joery, Snijders Blok, Lot, Fisher, Simon E, Kleefstra, Tjitske. 2022. Inherited variants in CHD3 show variable expressivity in Snijders Blok-Campeau syndrome. In Genetics in medicine : official journal of the American College of Medical Genetics, 24, 1283-1296. doi:10.1016/j.gim.2022.02.014. https://pubmed.ncbi.nlm.nih.gov/35346573/

2. Snijders Blok, Lot, Rousseau, Justine, Twist, Joanna, Fisher, Simon E, Campeau, Philippe M. 2018. CHD3 helicase domain mutations cause a neurodevelopmental syndrome with macrocephaly and impaired speech and language. In Nature communications, 9, 4619. doi:10.1038/s41467-018-06014-6. https://pubmed.ncbi.nlm.nih.gov/30397230/

3. Pascual, Patricia, Tenorio-Castano, Jair, Mignot, Cyril, Nevado, Julián, Lapunzina, Pablo. 2023. Snijders Blok-Campeau Syndrome: Description of 20 Additional Individuals with Variants in CHD3 and Literature Review. In Genes, 14, . doi:10.3390/genes14091664. https://pubmed.ncbi.nlm.nih.gov/37761804/

4. Hoffmeister, Helen, Fuchs, Andreas, Erdel, Fabian, Rippe, Karsten, Längst, Gernot. . CHD3 and CHD4 form distinct NuRD complexes with different yet overlapping functionality. In Nucleic acids research, 45, 10534-10554. doi:10.1093/nar/gkx711. https://pubmed.ncbi.nlm.nih.gov/28977666/

5. Gao, Yuanyuan, Wang, Pei, Chen, Mengying, Zhang, Hongmei, Zhu, Min. 2024. Novel genotypes and phenotypes in Snijders Blok-Campeau syndrome caused by CHD3 mutations. In Frontiers in genetics, 15, 1347933. doi:10.3389/fgene.2024.1347933. https://pubmed.ncbi.nlm.nih.gov/39050258/

6. Xie, Jun, Gao, Siqi, Schafer, Christopher, Muthukumar, Vijay, Griffin, Courtney T. 2020. The chromatin-remodeling enzyme CHD3 plays a role in embryonic viability but is dispensable for early vascular development. In PloS one, 15, e0235799. doi:10.1371/journal.pone.0235799. https://pubmed.ncbi.nlm.nih.gov/32658897/

7. Chen, Lin, Bu, Yanjiao, Yu, Yuwen, Chen, Yongxing, Lei, Xiaoguang. 2024. CHD3-related Snijders Blok-Campeau syndrome with Spastic Paraplegia, Ataxia, and Situs Inversus. In European journal of medical genetics, 73, 104988. doi:10.1016/j.ejmg.2024.104988. https://pubmed.ncbi.nlm.nih.gov/39709005/

8. LeBreton, Laure, Allain, Eric P, Parscan, Radu Christian, Almaghraby, Abdullah, Ben Amor, Mouna. 2022. A novel CHD3 variant in a patient with central precocious puberty: Expanded phenotype of Snijders Blok-Campeau syndrome? In American journal of medical genetics. Part A, 191, 1065-1069. doi:10.1002/ajmg.a.63096. https://pubmed.ncbi.nlm.nih.gov/36565043/

Quality Control Standard
Sperm Test

Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.

Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.

Environmental Standards:SPF
Available Region:Global
Source:Cyagen
Model Library
Model Library
Resources
Resources
Animal Quality
Animal Quality
Get Support
Get Support
Address:
2255 Martin Avenue, Suite E Santa Clara, CA 95050-2709, US
Tel:
800-921-8930 (8-6pm PST)
+1408-963-0306 (lnt’l)
Fax:
408-969-0338
Email:
animal-service@cyagen.com
service@cyagen.us
CRO Services
OncologyOphthalmologyNeuroscienceMetabolic & CardiovascularAutoimmune & InflammatoryGene TherapyAntibody Therapy
About Us
Corporate OverviewOur PartnersCareersContact Us
Social Media
Disclaimer: Pricing and availability of our products and services vary by region. Listed prices are applicable to the specific countries. Please contact us for more information.
Copyright © 2025 Cyagen. All rights reserved.
Privacy Policy
Site Map
Stay Updated with the Latest from Cyagen
Get the latest news on our research models, CRO services, scientific resources, and special offers—tailored to your research needs and delivered straight to your inbox.
Full Name
Email
Organization
Country
Areas of Interest